Purification of arachidonic acid from fungal single-cell oil via Al 2O3-supported CuSO4 column chromatography

JAOCS, Journal of the American Oil Chemists' Society

Volumn 83, Issue 7, 2006, Pages 659-662

  Zhang, Yun Xiao ^a   Tao, Jing Chao ^a   Wang, Le ^a  

^a ZHENGZHOU UNIVERSITY   (China)

Author keywords

Aluminum oxide; Arachidonic acid; Column chromatography; Cupric sulfate; Polyunsaturated fatty acids; Purification; Saponification; Single cell oil; Urea inclusion

Indexed keywords

ACETONE; ALUMINUM COMPOUNDS; CELLS; COLUMN CHROMATOGRAPHY; FUNGI; PURIFICATION; SAPONIFICATION; UREA;

ARACHIDONIC ACID; CUPRIC SULFATE; FUNGAL SINGLE-CELL OIL; HIGH-PURITY ARACHIDONIC ACID (AA); HUMAN NUTRIENT; POLYUNSATURATED FATTY ACIDS; SINGLE-CELL OIL; STATIONARY PHASE; UREA INCLUSION;

CARBOXYLIC ACIDS;

EID: 33746879607     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-006-1254-3     Document Type: Article

References (18)

1. Singh, M.D., and M.D. Chandra, Biochemical and Cellular Effects of Fish and Fish Oils, Prog. Food Nutr. Sci. 72:371-419 (1988).
2. Dyeberg, J., Linoleate-derived Polyunsaturated Fatty Acids and Prevention of Atherosclerosis. Nutr. Rev. 44:125-134 (1986).
3. Simopoulos, A.P., Omega-3 Fatty Acid in Health and Disease and in Growth and Development, Am. J. Clin. Nutr. 54:438-463 (1991).
4. Birch, E.E., D.R. Hoffman, R. Uauy, D.G. Birch, and C. Prestidge, Visual Acuity and the Essentiality of Docosahexaenoic Acid and Arachidonic Acid in the Diet of Term Infants, Pediatr. Res. 44:201-209 (1998).
5. Shinmen, Y., S. Shimizu, K. Akimoto, H. Kawashima, and H. Yamada, Production of Arachidonic Acid by Mortierella Fungi, Appl. Microbiol. Biotechnol. 31:11-16 (1989).
6. Baipai, P.K., P. Baipai, and O.P. Ward, Production of Arachidonic Acid by Mortierella alpina ATCC 32222, J. Ind. Microbiol. 5:179-185 (1991).
7. Guil-Guerrero, J.L., El-Hassan Belarbi, and M.M. Rebolloso-Fuentes, Eicosapentaenoic and Arachidonic Acids Purification from the Red Microalga Porphyridium cruentum, Bioseparation 9:299-306 (2000).
8. Shimada, Y., A. Sugihara, K. Maruyama, T. Nagao, S. Nakayama, H. Nakano, and Y. Tominaga, Enrichment of Arachidonic Acid: Selective Hydrolysis of a Single-Cell Oil from Mortierella with Candida cylindracea Lipase, J. Am. Oil Chem. Soc. 72:1323-1327 (1995).
9. Chen, T.C., and Y.H. Ju, Polyunsaturated Fatty Acid Concentrates from Borage and Linseed Oil Fatty Acids, J. Am. Oil Chem. Soc. Ibid. 75:485-488 (2001).
10. Hills, M.J., I. Kiewitt, and K.D. Mukherjee, Enzymatic Fraction of Fatty Acids: Enrichment of γ-Linolenic Acid and Docosoahexaenoic Acid by Selective Esteriflcation Catalyzed by Lipase, J. Am. Oil Chem. Soc. Ibid. 67:561-564 (1990).
11. McNeill, G.P., R.G. Ackman, and S.R. Moore, Lipase-Catalyzed Enrichment of Long-Chain Polyunsaturated Fatty Acids, J. Am. Oil Chem. Soc. Ibid. 75:1403-1407 (1996).
12. Vali, S.R., H.Y. Sheng, and Y.H. Ju, An Efficient Method for the Purification of Arachidonic Acid from Fungal Single-Cell Oil (ARASCO), J. Am. Oil Chem. Soc. Ibid. 80:725-130 (2003).
13. Ratnayake, W.M.N., B. Olsson, D. Matthews, and R.G. Ackman, Preparation of Omega-3 PUFA Concentrates from Fish Oil via Urea Complexation, Fat Sci. Technol. 70:381-386 (1988).
14. Scholfield, C.R., Silver Nitrate-High Performance Liquid Chromatography of Fatty Methyl Esters, J. Am. Oil Chem. Soc. 56:510-511 (1979).
15. Özcimder, M., and W.E. Hammers, Fractionation of Fish Oil Fatty Acid Methyl Esters by Means of Argentation and Reversed-Phase High Performance Liquid Chromatography, and Its Utility in Total Fatty Acid Analysis, J. Chromatogr. A 187:307-317 (1980).
16. 3-Processed Silica Gel Chromatography in Isolation and Purification of Active Components of Herbal Medicine, J. Guangzhou University of Traditional Chinese Medicine 27:319-321 (2004).
17. Robles Medina, A., A. Giménez Giménez, F. García Camacho, J.A. Sánchez Pérez, E. Molina Grima, and A. Contreras Gómez, Concentration and Purification of Stearidonic, Eicosapentaenoic, and Docosahexaenoic Acids from Cod Liver Oil and the Marine Microalga Isochrysis galbana, J. Am. Oil Chem. Soc. 72:575-583 (1995).
18. Yuan, C.L., J. Zhang, X.Q. Wang, and Z.L. Yu, Isolation and Purification of Arachidonic Acid in Microbial Lipids by High Performance Liquid Chromatography, Chin. J. Chromatogr. 27:60-62 (2003).