Identification of glutamate residues important for catalytic activity or thermostability of a truncated Bacillus sp. strain TS-23 α-amylase by site-directed mutagenesis

Protein Journal

Volumn 25, Issue 3, 2006, Pages 232-239

  Lin, Long Liu ^a   Chen, Pei Jing ^b   Liu, Jai Shin ^c   Wang, Wen Ching ^c   Lo, Huei Fen ^b  

^a NATIONAL CHIAYI UNIVERSITY   (Taiwan)

^b HUNGKUANG UNIVERSITY   (Taiwan)

^c NATIONAL TSING HUA UNIVERSITY   (Taiwan)

Author keywords

Bacillus sp. strain TS 23; Catalytic reaction; Site directed mutagenesis; Thermostability; Amylase

Indexed keywords

AMYLASE; BACTERIAL ENZYME; GLUTAMIC ACID; NICKEL COMPLEX;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACTERIAL CELL; BACTERIAL STRAIN; CATALYSIS; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STATISTICAL SIGNIFICANCE; TEMPERATURE; THERMOSTABILITY; WILD TYPE;

BACILLUS SP. TS-23;

EID: 33746804090     PISSN: 15723887     EISSN: 18758355     Source Type: Journal    
DOI: 10.1007/s10930-006-9006-7     Document Type: Article

References (34)

1. Boel, E., Brady, L., Brzozowski, A. M., Derewenda, Z., Dodson, G. G., Jensen, V. J., Petersen, S. B., Swift, H., Thim, L., and Woldike, H. F. (1990). Biochemistry 29: 6244-6249.
2. Bradford, M. M. (1976). Anal. Chem. 72: 248-254.
3. Buisson, G., Duee, E., Haser, R., and Payan, F. (1987). EMBO J. 6: 3909-3916.
4. Chang, C. T., Lo, H. F., Chi, M. C., Yao, C. Y., Hsu, W. H., and Lin, L. L. (2003). Extremophiles 7: 505-509.
5. Declerck, N., Machius, M., Wiegand, G., Huber, R., and Gaillardin, C. (2000). J. Mol. Biol. 301: 1041-1057.
6. Guzman-Maldonado, H., and Paredes-Lopez, O. (1995). Crit. Rev. Food Sci. Nutr. 35: 373-403.
7. Henrissat, B. (1991). Biochem. J. 280: 309-316.
8. Ito, S., Kobayashi, T., Ara, K., Ozaki, K., Kawai, S., and Hatada, Y. (1998). Extremophiles 2: 185-190.
9. Kang, Y. N., Adachi, M., Utsumi, S., and Mikami, B. (2004). J. Mol. Biol. 339: 1129-1140.
10. Karn, R. C. (1978). Adv. Comp. Physiol. Biochem. 7: 1-103.
11. Katsuya, Y., Mezaki, Y., Kubota, M., and Matsuura, Y. (1998). J. Mol. Biol. 281: 885-897.
12. Kim, J. S., Chan, S. S., Kim, H. J., Kim, T. J., Ha, N. C., Oh, S. T., Cho, H. S., Cho, M. J., Kim, M. J., Lee, H. S., Kim, J. W., Choi, K. Y., Park, K. H., and Oh, B. H. (1999). J. Biol. Chem. 274: 26279-26286.
13. Kossmann, J., and Lloyd, J. (2000). Crit. Rev. Biochem. Mol. Biol. 35: 141-196.
14. Kuriki, T., and Imanaka, T. (1999). J. Biosci. Bioeng. 87: 557-565.
15. Laemmli, U. K. (1970). Nature (Lodon) 227: 680-685.
16. Lawson, C. L., van Montfort, R., Strokopytov, B., Rozeboom, H. J., Kalk, K. H., de Vries, G., Penninga, D., Dijkhuizen, L., and Dijkstra, B. W. (1994). J. Mol. Biol. 236: 590-600.
17. Lehmann, M., Pasamontes, L., Lassen, S. F., and Wyss, M. (2000). Biochim. Biophys. Acta 1543: 408-415.
18. Li, F., and Mullins, J. I. (2002). Methods Mol. Biol. 182: 19-27.
19. Lin, L. L., Hsu, W. H., and Chu, W. S. (1997). J. Appl. Microbiol. 82: 325-334.
20. Lin, L. L., Tsau, M. R., and Chu, W. S. (1994). Appl. Microbial. Biotechnol. 42: 51-56.
21. Lo, H. F., Lin, L. L., Li, C. C., Hsu, W. H., and Chang, C. T. (2001). Curr. Microbiol. 43: 170-175.
22. Lo, H. F., Lin, L. L., Chiang, W. Y., Chi, M. C., Hsu, W. H., and Chang, C. T. (2002). Arch. Microbiol. 178: 115-123.
23. Machius, M., Wiegand, G., and Huber, R. (1995). J. Mol. Biol. 246: 545-559.
24. Matsuura, Y., Kusunoki, M., Harada, W., and Kakudo, M. (1984). J. Biochem (Tokyo) 95: 697-702.
25. Miller, G. L. (1959). Anal. Chem. 31: 426-428.
26. Morishita, Y., Hasegawa, K., Matsuura, Y., Katsuya, Y., Kubato, M., and Sakai, S. (1997). J. Mol. Biol. 267: 661-672.
27. Ohdan, K., Kuriki, T., Kaneko, H., Shimada, J., Takada, T., Fujimoto, Z., Mizuno, H., and Okada, S. (1999). Appl. Environ. Microbiol. 65: 4652-4658.
28. Qian, M., Haser, R., and Payan, F. (1993). J. Mol. Biol. 231: 758-799.
29. Sambrook, J., and Russell, D. W. (2001) In: Molecular Cloning: A Laboratory Manual, Cold spring harbor laboratory Press, New York, pp. 1.31-1.131.
30. Sarcabal, P., Remaud-Simeon, M., Willemot, R., Potockide Montalk, G., Svensson, B., and Monsan, P. (2000). FEBS Lett. 474: 33-37.
31. Strokopytov, B., Knegtel, R. M. A., Penninga, D., Roozeboom, H. J., Kalk, K. H., Dijkhuizen, L., and Dijkstra, B. W. (1996). Biochemistry 35: 4241-4249.
32. Uitdehaag, J. C. M., Mosi, R., Kalk, K. H., van der Veen, B. A., Dijkhuizen, L., Withers, S. G., and Dijkstra, B. W. (1999). Nat. Struct. Biol. 6: 432-436.
33. Vihinen, M., and Mäntsälä, P. (1989). Crit. Rev. Biochem. Mol. Biol. 24: 329-418.
34. Watanabe, K., Miyake, K., and Suzuki, Y. (2001). Biosci. Biotechnol. Biochem. 65: 2058-2064.