Effects of temperature and aldehyde fixation on tissue water diffusion properties, studied in an erythrocyte ghost tissue model

Magnetic Resonance in Medicine

Volumn 56, Issue 2, 2006, Pages 282-289

  Thelwall, Peter E ^a,d   Shepherd, Timothy M ^a   Stanisz, Greg J ^b   Blackband, Stephen J ^a,c  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TORONTO   (Canada)

^c FLORIDA STATE UNIVERSITY   (United States)

^d NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Diffusion analysis; Diffusion weighted imaging; Fixative; Formaldehyde; Glufaraldehyde; Water exchange

Indexed keywords

BLOOD; DIFFUSION IN LIQUIDS; FORMALDEHYDE; MAGNETIC RESONANCE IMAGING; TISSUE;

DIFFUSION ANALYSIS; DIFFUSION WEIGHTED IMAGING; FIXATIVE; GLUFARALDEHYDE; WATER EXCHANGE;

TEMPERATURE;

AGAROSE; ALDEHYDE; BUFFER; FIXATIVE; FORMALDEHYDE; GLUTARALDEHYDE; WATER;

ARTICLE; BIOPHYSICS; CELL SUSPENSION; DIFFUSION COEFFICIENT; DIFFUSION WEIGHTED IMAGING; ERYTHROCYTE; ERYTHROCYTE GHOST TISSUE MODEL; EX VIVO STUDY; HUMAN; HUMAN CELL; IN VIVO STUDY; MAGNET; MEMBRANE PERMEABILITY; NUCLEAR MAGNETIC RESONANCE; SPECTROMETER; TEMPERATURE; TISSUE FIXATION; WATER TRANSPORT;

EID: 33746706896     PISSN: 07403194     EISSN: 15222594     Source Type: Journal    
DOI: 10.1002/mrm.20962     Document Type: Article

References (31)

1. Hirsch WL, Kemp SS, Martinez AJ, Curtin H, Latchaw RE, Wolf G. Anatomy of the brainstem: correlation of in vitro MR images with histologic sections. AJNR Am J Neuroradiol 1989;10:923-928.
2. Fatterpekar GM, Naidich TP, Delman BN, Aguinaldo JG, Gultekin SH, Sherwood CC, Hof PR, Drayer BP, Fayad ZA. Cytoarchitecture of the human cerebral cortex: MR microscopy of excised specimens at 9.4 Tesla. AJNR Am J Neuroradiol 2002;23:1313-1321.
3. Padgett KR, Maria BL, Yachnis AT, Blackband SJ. Ex vivo high-resolution magnetic resonance imaging of the brain in Joubert's syndrome. J Child Neurol 2002;17:911-913.
4. Pfefferbaum A, Sullivan EV, Adalsteinsson E, Garrick T, Harper C. Postmortem MR imaging of formalin-fixed human brain. Neuroimage 2004;21:1585-1595.
5. Hsu JC, Johnson GA, Smith WM, Reimer KA, Ideker RE. Magnetic resonance imaging of chronic myocardial infarcts in formalin-fixed human autopsy hearts. Circulation 1994;89:2133-2140.
6. Dalager-Pedersen S, Falk E, Ringgaard S, Kristensen IB, Pedersen EM. Effects of temperature and histopathologic preparation on the size and morphology of atherosclerotic carotid arteries as imaged by MRI. J Magn Reson Imaging 1999;10:876-885.
7. Holmes AA, Scollan DF, Winslow RL. Direct histological validation of diffusion tensor MRI in formaldehyde-fixed myocardium. Magn Reson Med 2000;44:157-161.
8. Benveniste H, Blackband S. MR microscopy and high resolution small animal MRI: applications in neuroscience research. Prog Neurobiol 2002;67:393-420.
9. Benveniste H, Mareci TH, Blackband SJ. Anatomical studies in the rodent brain and spinal cord: applications of magnetic resonance microscopy. In: van Bruggen N, Roberts T, editors. Techniques of biomedical imaging in neuroscience. Boca Raton: CRC Press, 2003. p 211-235
10. Johnson GA, Gofer GP, Fubara B, Gewalt SL, Hedlund LW, Maronpot RR. Magnetic resonance histology for morphologic phenotyping. J Magn Reson Imaging 2002;16:423-429.
11. Johnson GA, Cofer GP, Gewalt SL, Hedlund LW. Morphologic phenotyping with MR microscopy: the visible mouse. Radiology 2002;222: 789-793.
12. Zhang J, Richards LJ, Yarowsky P, Huang H, van Zijl PC, Mori S. Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging. Neuroimage 2003;20: 1639-1648.
13. Zhang J, Chen YB, Hardwick JM, Miller MI, Plachez C, Richards LJ, Yarowsky P, van Zijl P, Mori S. Magnetic resonance diffusion tensor microimaging reveals a role for Bcl-x in brain development and homeostasis. J Neurosci 2005;25:1881-1888.
14. Tovi M, Ericsson A. Measurements of T1 and T2 over time in formalin-fixed human whole-brain specimens. Acta Radiol 1992;33:400-404.
15. Thelwall PE, Shepherd TM, Stanisz GJ, Blackband SJ. Effect of fixative osmolality on biological tissue microstructure studied with an erythrocyte ghost model. In: Proceedings of the 12th Annual Meeting of ISMRM, Kyoto, Japan, 2004. p 1233.
16. Kiernan JA. Histological and histochemical methods: theory and practice. Oxford: Butterworth-Heinemann; 1999. 502 p.
17. Thelwall PE, Grant SC, Stanisz GJ, Blackband SJ. Human erythrocyte ghosts: Exploring the origins of multiexponential water diffusion in a model biological tissue with magnetic resonance. Magn Reson Med 2002;48:649-657.
18. Li JG, Stanisz GJ, Henkelman RM. Integrated analysis of diffusion and relaxation of water in blood. Magn Reson Med 1998;40:79-88.
19. Wood PG. Preparation of white resealable erythrocyte ghosts. Methods Enzymol 1987;149:271-280.
20. Van Geet AL. Calibration of the methanol and glycol nuclear magnetic resonance thermometers with a static thermistor probe. Anal Chem 1968;40:2227-2229.
21. Stejskal EO, Tanner JE. Spin diffusion measurements: spin echos in the presence of a time-dependent field gradient. J Chem Phys 1965;42: 3597-3603.
22. Mills R. Self-diffusion in normal and heavy water in the range 1-45 C. J Phys Chem 1973;77:685-688.
23. Morvan D, Leroy-Willig A. Simultaneous measurements of diffusion and transverse relaxation in exercising skeletal muscle. Magn Reson Imaging 1995;13:943-948.
24. Hasegawa Y, Latour LL, Sotak CH, Dardzinski BJ, Fisher M. Temperature dependent change of apparent diffusion coefficient of water in normal and ischemic brain of rats. J Cereb Blood Flow Metab 1994;14: 383-390.
25. Benga G, Pop VI, Popescu O, Borza V. On measuring the diffusional water permeability of human red blood cells and ghosts by nuclear magnetic resonance. J Biochem Biophys Methods 1990;21:87-102.
26. Hahnenstein I, Albert M, Hasse H, Kreiter C, Maurer G. NMR spectroscopic and densimetric study of reaction-kinetics of formaldehyde polymer formation in water, deuterium-oxide, and methanol. Indust Eng Chem Res 1995;34:440-450.
27. Bossart EL, Inglis BA, Silver XS, Mareci TH. The effect of fixative solutions in magnetic resonance imaging. In: Proceedings of the 7th Annual Meeting of ISMRM, Philadelphia, PA, USA, 1999. p 1928.
28. Zeuthen T. How water molecules pass through aquaporins. Trends Biochem Sci 2001;26:77-79.
29. Sun SW, Neil JJ, Song SK. Relative indices of water diffusion anisotropy are equivalent in live and formalin-fixed mouse brains. Magn Reson Med 2003;50:743-748.
30. Sun SW, Neil JJ, Liang HF, He YY, Schmidt RE, Hsu CY, Song SK. Formalin fixation alters water diffusion coefficient magnitude but not anisotropy in infarcted brain. Magn Reson Med 2005;53:1447-1451.
31. Shepherd TM, Thelwall PE, Stanisz GJ, Blackband SJ. Chemical fixation alters the water microenvironment in rat cortical brain slices-implications for MRI contrast mechanisms. In: Proceedings of the 13th Annual Meeting of ISMRM, Miami, FL, USA. 2005. p 619.