Examining substrate uptake patterns of Rhodocyclus-related PAO in full-scale EBPR plants by using the MAR-FISH technique

Water Science and Technology

Volumn 54, Issue 1, 2006, Pages 63-70

  Chua, Adeline S M ^a   Onuki, M ^a   Satoh, H ^a   Mino, T ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Aspartate; Enhanced biological phosphorus removal; Glutamate; Microautoradiography fluorescent in situ hybridization; Polyphosphate accumulating organisms; Rhodocyclus

Indexed keywords

ASPARTATE; ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL; GLUTAMATE; MICROAUTORADIOGRAPHY - FLUORESCENT IN SITU HYBRIDIZATION; POLYPHOSPHATE ACCUMULATING ORGANISMS; RHODOCYCLUS;

ACTIVATED SLUDGE PROCESS; AMINO ACIDS; PHOSPHORUS; RADIOGRAPHY; REMOVAL; SUBSTRATES;

PHYSIOLOGY;

4',6 DIAMIDINO 2 PHENYLINDOLE; ACETIC ACID; AMINO ACID; ASPARTIC ACID; CARBON; GLUTAMIC ACID; PALMITIC ACID; PHOSPHORUS; POLYPHOSPHATE;

ACTIVATED SLUDGE PROCESS; AMINO ACIDS; PHOSPHORUS; PHYSIOLOGY; RADIOGRAPHY; REMOVAL; SUBSTRATES;

ASPARTIC ACID; BIOREACTOR; FLUORESCENCE SPECTROSCOPY; HYBRIDIZATION; PHYSIOLOGY; SUBSTRATE;

ACTIVATED SLUDGE; AEROBIC METABOLISM; ANAEROBIC METABOLISM; ARTICLE; AUTORADIOGRAPHY; CONTROLLED STUDY; EPIFLUORESCENCE MICROSCOPY; FLUORESCENCE IN SITU HYBRIDIZATION; MICROAUTORADIOGRAPHY; MICROBIAL BIOMASS; NONHUMAN; RHODOCYCLACEAE; WASTE WATER; WASTE WATER TREATMENT PLANT;

ENVIRONMENTAL REMEDIATION; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, FLUORESCENCE; PHOSPHORUS; RHODOCYCLACEAE; SEWAGE;

RHODOCYCLUS;

EID: 33746606423     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2006.372     Document Type: Article

References (19)

1. Amann, R.I. (1995). In situ identification of microorganisms by whole cell hybridization with rRNA-targeted nucleic acid probes. Molecular Microbial Ecological Manual, Akkermans, A.D.L., van Elsas, J.D. and de Bruijn, F.J. (eds), Kluwer Academic, London, United Kingdom, part 3.3.6. pp. 1-15.
2. Chua, A.S.M., Eales, K., Mino, T. and Seviour, R. (2004). The large PAO cells in full-scale EBPR biomass samples are not yeast spores but possibly novel members of the beta-Proteobacteria. Wat. Sci Tech., 50(6), 123-130.
3. Crocetti, G.R., Hugenholtz, P., Bond, P.L., Schuler, A., Keller, J., Jenkins, D. and Blackall, L.L. (2000). Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl. Environ. Microbiol., 66(3), 1175-1182.
4. Fuhs, G.W. and Chen, M. (1975). Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microb. Ecol., 2, 119-138.
5. Hesselmann, R.P., Werlen, C., Hahn, D., van der Meer, J.R. and Zehnder, A.J. (1999). Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst. Appl. Microbiol., 22(3), 454-465.
6. Kawaharasaki, M., Tanaka, H., Kanagawa, T. and Nakamura, K. (1999). In situ identification of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4′, 6-diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration. Wat. Res., 33(1), 257-265.
7. Kong, Y., Beer, M., Rees, G.N. and Seviour, R.J. (2002). Functional analysis of microbial communities in aerobic-anaerobic sequencing batch reactors fed with different phosphorus/carbon (P/C) ratios. Microbiology, 148(8), 2299-2307.
8. Kong, Y., Nielsen, J.L. and Nielsen, P.H. (2004). Microautoradiographic study of Rhodocyclus-related polyphosphate accumulating bacteria in full-scale enhanced biological phosphorus removal plants. Appl. Environ. Microbiol., 70(9), 5383-5390.
9. Lee, N., Nielsen, P.H., Andreasen, K.H., Juretschko, S., Nielsen, J.L., Schleifer, K.H. and Wagner, M. (1999). Combination of fluorescent in situ hybridization and microautoradiography - a new tool for structure-function analyses in microbial ecology. Appl. Environ. Microbiol., 65(3), 1289-1297.
10. Lee, N., Jansen, J.C., Aspegren, H., Henze, M., Nielsen, P.H. and Wagner, M. (2002). Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal. Wat. Sci. Tech., 46(1-2), 163-170.
11. Liu, W.T., Nielsen, A.T., Wu, J.H., Tsai, C.S., Matsuo, Y. and Molin, S. (2001). In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ. Microbiol., 3, 110-122.
12. Loy, A., Horn, M. and Wagner, M. (2003). probeBase - an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res., 31, 514-516.
13. Mino, T., van Loosdrecht, M.C.M. and Heijnen, J.J. (1998). Microbiology and biochemistry of enhanced biological phosphate removal process. Wat. Res., 32(11), 3193-3207.
14. Okabe, S., Kindaichi, T. and Ito, T. (2004). MAR-FISH-An ecophysiological approach to link phylogenetic affiliation and in situ metabolic activity of microorganisms at a single-cell resolution. Microb. Environ., 19(2), 83-98.
15. Onuki, M., Satoh, H., Mino, T. and Matsuo, T. (2000). Application of molecular methods microbial community analysis of activated sludge. Wat. Sci. Tech., 42(3-4), 17-22.
16. Onuki, M., Satoh, H. and Mino, T. (2002). Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate. Wat. Sci. Tech., 46(1-2), 145-154.
17. Satoh, H., Mino, T. and Matsuo, T. (1998). Anaerobic uptake of glutamate and aspartate by enhanced biological phosphorus removal activated sludge. Wat. Sci. Tech., 37(4-5), 579-582.
18. Seviour, R.J., Mino, T. and Onuki, M. (2003). The microbiology of biological phosphorus removal in activated sludge systems. FEMS Microbiol. Rev., 27, 99-127.
19. Zilles, J.L., Hung, C.H.. and Noguera, D.A. (2002). Presence of Rhodocyclus in. a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal. Wat. Sci. Tech., 46(1-2), 123-128.