A Dehydration Method for Immature or Wet Cotton Fibers for Light and Electron Microscopy

Textile Research Journal

Volumn 76, Issue 6, 2006, Pages 514-518

  Rajasekaran, Kanniah ^a   Muir, Alan J ^a   Ingber, Bruce F ^a   French, Alfred D ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

2,2 dimethoxypropane; Cell wall; fiber quality; maturity; moisture; thin sections

Indexed keywords

DEHYDRATION; DRYING; ELECTRON MICROSCOPY; MOISTURE; OPTICAL MICROSCOPY; RESINS;

CELL WALL; DIMETHOXYPROPANE (DMP); ULTRASTRUCTURAL INTEGRITY; WET COTTON FIBERS;

COTTON FIBERS;

COTTON FIBER; CROSS SECTION; MATURITY; MOISTURE; SCANNING ELECTRON MICROSCOPY;

EID: 33746603012     PISSN: 00405175     EISSN: None     Source Type: Journal    
DOI: 10.1177/0040517506063390     Document Type: Article

References (17)

1. Bailey, Jr, T. L. W., and Rollins, M. L., Some Applications of Microscopy to Cotton Research, Textile Res. J. 15, 1-6 (1945).
2. Boylston, E. K., Enhanced Preparation of Cotton Cross-sections for Image Analysis, “Proceedings of the Beltwide Cotton Conference, 1995,” National Cotton Council of America, Memphis, TN, pp. 1289-1290, (1995).
3. Boylston, E. K., Evans, J. P., and Thibodeaux, D. P., A Quick Embedding Method for Light Microscopy and Image Analysis of Cotton Fibers, Biotechnic. & Histochem. 70, 24-27 (1995).
4. Tripp, V. W., Some Applications of Light Microscopy in the Study of Textile Materials, Am. Soc. Testing Mats., Spec. Tech. Pub. 143, 25-34 (1952).
5. Goynes, W. R., Ingber, B. F., and Triplett, B. A., Cotton Fiber Secondary Wall Development-time versus Thickness, Textile Res. J. 65, 400-408 (1995).
6. Backe, E. E., “Cotton Moisture: Harvesting through Textile Processing,” Institute of Textile Technology, Charlottesville, VA, 2002.
7. Jacks, T. J., Muller, L. L., and Evans, W. J., Time Requirement for Chemical Dehydration of Tissues with 2,2-Dimethoxypro-pane for Microscopy, Microscope 29, 137-140 (1981).
8. Muller, L. L., and Jacks, T. J., Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations, J. Histochem. Cytochem. 23, 107-110 (1975).
9. Newman, S. B., Borysko, E., and Swerdlow, M., New Sectioning Techniques for Light and Electron Microscopy, Science 110, 66-68 (1949).
10. Spurr, A. R., A Low-viscosity Epoxy Resin Embedding Medium for Electron Microscopy, J. Ultrastruct. Res. 26, 31-43 (1969).
11. Rajasekaran, K., and Thibodeaux, D. P., Fidelity of Cotton Fiber Maturity Measurement by Image Analysis, J. New Seeds 4(3), 25-36 (2002).
12. Yatsu, L. Y., and Jacks, T. J., Fixation of Cotton (Gossypium hirsutum L.) Hair Cells (fibers). Microscope 27, 113-116 (1979).
13. Yatsu, L. Y., and Jacks, T. J., An Ultrastructural Study of the Relationship between Microtubules and Microfibrils in Cotton (Gossypium hirsutum L.) Cell Wall Reversals. Am. J. Bot. 68, 771-777 (1981).
14. Boylston, E. K., Hinojosa, O., and Hebert, J. J., A Quick Embedding Method for Light and Electron Microscopy of Textile Fibers, Biotechnic. & Histochem. 66, 122-124 (1991).
15. Thibodeaux, D. and Rajasekaran, K., Development of New Reference Standards for Cotton Fiber Maturity, J. Cotton Sci. 3, 188-193 (1999). http://www.jcotsci.org/1999/issue04/xNote/art01/article.pdf
16. Nemanic, M., Critical Point Drying, Cryofracture and Serial Sectioning, in “Scanning Electron Microscopy,” IITRI, Chicago, IL, pp. 297-304, 1972.
17. Reynolds, E. S., The Use of Lead Citrate at High pH as an Electron Opaque Stain in Electron Microscopy, J. Cell Biol. 17, 208-212 (1963).