Structural basis of the destabilization produced by an amino-terminal tag in the β-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus

Biochimie

Volumn 88, Issue 7, 2006, Pages 807-817

  Ausili, A ^a   Cobucci Ponzano, B ^b   Di Lauro, B ^b   D'Avino, R ^b   Scire A ^a   Rossi, M ^b,c   Tanfani, F ^a   Moracci, M ^b  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Archaea; Glycoside hydrolase; Infrared spectroscopy; Quaternary structure; Thermal stability

Indexed keywords

BETA GLYCOSIDASE; ENZYME; GLYCOSIDASE; POLYPEPTIDE; UNCLASSIFIED DRUG; ARCHAEAL PROTEIN; GLUCOSIDASE; RECOMBINANT PROTEIN; SULFOLOBUS SOLFATARICUS BETA GLYCOSIDASE; SULFOLOBUS SOLFATARICUS BETA-GLYCOSIDASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CORRELATION ANALYSIS; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME STRUCTURE; EXPERIMENTAL STUDY; HYPOTHESIS; INFRARED RADIATION; INFRARED SPECTROSCOPY; MOLECULAR MODEL; PROTEIN QUATERNARY STRUCTURE; RNA TRANSLATION; STRUCTURE ANALYSIS; SULFOLOBUS SOLFATARICUS; THERMOPHILIC BACTERIUM; THERMOSTABILITY; WILD TYPE; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; GENETICS; HEAT; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTATION; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; TEMPERATURE; TIME;

SULFOLOBUS SOLFATARICUS;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; ENZYME STABILITY; GLUCOSIDASES; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; STRUCTURE-ACTIVITY RELATIONSHIP; SULFOLOBUS SOLFATARICUS; TEMPERATURE; TIME FACTORS;

EID: 33746513906     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2006.01.009     Document Type: Article

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