Flow cytometric quantification of antigen D sites on red blood cells of partial D and weak D variants in India

Transfusion Medicine

Volumn 16, Issue 4, 2006, Pages 285-289

  Kulkarni, S ^a   Mohanty, D ^a   Gupte, S ^b   Vasantha, K ^a   Joshi, S ^c  

^a KEM HOSPITAL   (India)

^b Surat Raktadan Kendra and Research Centre   (India)

^c Indian Red Cross Society   (India)

Author keywords

D antigenic sites; Flow cytometry; Partial D; Weak D

Indexed keywords

EPITOPE; ERYTHROCYTE ANTIGEN;

ANTIGEN DETECTION; ANTIGENICITY; ARTICLE; CALCULATION; ERYTHROCYTE; FLOW CYTOMETRY; IMMUNOFLUORESCENCE; INDIA; PHENOTYPE; POPULATION; QUANTITATIVE ANALYSIS; SEROLOGY; STANDARD;

ANTIBODIES, MONOCLONAL; BINDING SITES; ERYTHROCYTES; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; INDIA; ISOANTIBODIES; RH-HR BLOOD-GROUP SYSTEM; VARIATION (GENETICS);

EID: 33746453778     PISSN: 09587578     EISSN: 13653148     Source Type: Journal    
DOI: 10.1111/j.1365-3148.2006.00667.x     Document Type: Article

References (18)

1. Dirk, R., van Bockstaele, D.R., Berneman, Z.N., Muylle, L., Cole-Dergent, J. & Peetermans, M.E. (1986) Flow cytometric analysis of erythrocyte D antigen density profile. Vox Sanguinis, 51, 40-46.
2. Faas, B.H.W., Beckers, E.A.M., Maaskant-van-Wijk, P.A., Overbeeke, M.A.M. & Van Rhenen, D.J. (1997) Molecular characterization of quantitative Rh variants. Biotest Bulletin, 5, 439-449.
3. Flegel, W.A., Curin-Serbec, V., Delamaire, M., Donvito, B. & Ikeda, H. (2002) Rh flowcytometry: coordinators report phases index and antigen density: on analysis of the flowcytometric determination. Transfusion Clinique et Biologique, 9, 33-42.
4. Flegel, W.A. & Wagner, F.F. (1996). RhD epitopes density profiles of RhD variants red cells analysed by flowcytometry. Transfusion Clinique et Biologique, 6, 425-431.
5. Fletcher, A. & Bryant, J.A. (1997) The analysis of Rh phenotypes by flow cytometry. Biotest Bulletin, 5, 495-502.
6. Freedman, J. & Alan, D. (1995) Application of flow cytrometry in transfusion medicine. Transfusion Medical Review, IX, 87-107.
7. Gorick, B., McDougall, D.C.J., Ouwehand, W.H., Overbeeke, M.A.M., Tippett, P., Hughes Jones, N.C. & van Rhenen, D.J. (1993) Quantitation of D sites on selected 'weak D' and partial D red cells. Vox Sanguinis, 65, 136-140.
8. u and Del. Transfusion, 30, 236-238.
9. Jones, J.W., Lloyd-Evans, P. & Kumpel, B.M. (1996). Quantitation of Rh D antigen sites on weak D and D variant red cell by flow cytometry. Vox Sanguinis, 71, 176-183.
10. Kulkarni, S., Colah, R., Gorakshakar, A., Gupte, S. & Mohanty, D. (2004) Detection and molecular characterization of partial D variants in India. Vox Sanguinis, 87 (Suppl. 3), 131.
11. Kumpel, B.M. (1990) A simple non isotopic method for the quantitation of red cell bound immunoglobulin. Vox Sanguinis, 59, 34-38.
12. Maaskant-van Wijk, P.A., Faas, B.H.W., de Ruijter, J.A.M., Overbeeke, M.A., von dem Borne A.E., van Rhenen, D.J. & van der School, C.E. (1998) Genotyping of RHD by multiplex polymerase chain reaction analysis of six RHD specific exons. Transfusion, 38, 1015-1021.
13. Nicholson, G., Lawrence, A., Ala, F.A. & Bird, G.W.G. (1991) Semi quantitative assay of D antigen site density by flow cytometric analysis. Transfusion Medicine, 1, 87-90.
14. Rochana, B. & Hughes Jones, N.C. (1965) The use of purified 125, I labeled anti-gamma globulin in the determination of number of D antigen sites on red cells of different phenotypes. Vox Sanguinis, 10, 675-686.
15. Tazzari, P.L., Bontadini, A., Belletti, D., Malferrari, F. & Conte, R. (1994) Flow cytometry: a toological in immunohaematology for D + W (Du)antigen evaluation. Vox Sanguinis, 67, 382-386.
16. Wagner, F.F. (1994) Influence of Rh phenotype on the antigen density of C, c and D: flow cytometric study using a frozen standard red cell. Transfusion, 34, 671-676.
17. Wagner, F.F., Frohmajer, A., Ladewig, B., Eicher, N., Lonicer, C.B., Muller, T.H., Siegel, M.H. & Flegel, W.A. (2000) Weak D alleles express distinct phenotypes. Blood, 95, 2699-2708.
18. Wagner, F.F., Gassner, C., Muller, T.H., Schonitzer, D., Schunter, F. & Flegel, W.A. (1998) Three molecular structures cause rhesus D category VI phenotypes with distinct immunohaematologic features. Blood, 91, 2157-2168.