Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans

Applied Microbiology and Biotechnology

Volumn 71, Issue 5, 2006, Pages 654-660

  Arai, Takamitsu ^a   Kosugi, Akihiko ^b   Chan, Helen ^a   Koukiekolo, Roger ^c   Yukawa, Hideaki ^d   Inui, Masayuki ^d   Doi, Roy H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b JAPAN INTERNATIONAL RESEARCH CENTER FOR AGRICULTURAL SCIENCES   (Japan)

^c SIMS   (Canada)

^d RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; CATALYST ACTIVITY; CATALYSTS; CELLULOSE; CHROMATOGRAPHY; CLONING; ENZYME KINETICS; GENES; GENETIC ENGINEERING; SOLUBILITY;

CARBOHYDRATE-BINDING MODULE (CBM); CARBOXYMETHYLCELLULOSE (CMC); CELLULOSOMAL CELLULASES; THIN-LAYER CHROMATOGRAPHY;

ENZYMES;

BACTERIAL ENZYME; CELLULASE; CELLULOSOME; MICROCRYSTALLINE CELLULOSE;

BACTERIUM; CELLULOSE; ENZYME; ENZYME ACTIVITY;

ACIDITY; ARTICLE; BINDING KINETICS; BIOTECHNOLOGY; CATALYSIS; CLOSTRIDIUM CELLULOVORANS; COMPARATIVE STUDY; DEGRADATION KINETICS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; PROTEIN FAMILY; SOLUBILITY; THIN LAYER CHROMATOGRAPHY;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CELLULASE; CELLULOSE; CLOSTRIDIUM CELLULOVORANS; MULTIENZYME COMPLEXES; SUBSTRATE SPECIFICITY;

CLOSTRIDIUM CELLULOVORANS;

EID: 33746341808     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0249-6     Document Type: Article

References (26)

1. Arai T, Ohara H, Karita T, Kimura T, Sakka K, Ohmiya K (2001) Sequence of celQ and properties of Ce1Q, a component of the Clostridium thermocellum cellulosome. Appl Microbiol Biotechnol 57:660-666
2. Arai T, Araki R, Tanaka A, Karita S, Kimura T, Sakka K, Ohmiya K (2003) Characterization of a cellulase containing a family 30 carbohydrate-binding module (CBM) derived from Clostridium thermocellum CelJ: importance of the CBM to cellulose hydrolysis. J Bacteriol 185:504-512
3. Bayer EA, Morag E, Lamed R, Yaron S, Shoham Y (1998a) Cellulosome structure; four-pronged attack using biochemistry, molecular biology, crystallography and bioinformatics. In: Claeyssens M, Nerinckx W, Piens K (eds) Carbohydrate from Trichoderma reesei and other microorganisms. Bookcraft, Bath, UK, pp 39-65
4. Bayer EA, Shimon LJW, Lamed R, Shoham Y (1998b) Cellulosomes: structure and ultrastructure. J Struct Biol 124:221-234
5. Béguin P, Cornet P, Aubert JP (1985) Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum. J Bacteriol 162:102-105
6. Belaich A, Parsiegla G, Gal L, Villard C, Haser R, Belaich JP (2002) Cel9M, a new family 9 cellulase of Clostridium cellulolyticum cellulosome. J Bacteriol 184:1378-1384
7. Doi RH, Kosugi A (2004) Cellulosomes: plant-cell-wall-degrading enzyme, complexes. Nat Rev Microbiol 2:541-551
8. Doi RH, Kosugi A, Murashima K, Tamaru Y, Han SO (2003) Cellulosomes from mesophilic bacteria. J Bacteriol 185:5907-5914
9. Dygert S, Li LH, Don Florida R, Thoma JA (1965) Determination of reducing sugar with improved precision. Anal Chem 13:367-374
10. Gal L, Gaudin C, Belaich A, Pages S, Tardif C, Belaich JP (1997) CelG from Clostridium cellulolyticum: a multidomain endoglucanase acting efficiently on crystalline cellulose. J Bacteriol 179:6595-6601
11. Gasparic J, Churacek J (1978) Laboratory handbook of paper and thin-layer chromatography. Ellis Horwood, Chichester, pp 153-154
12. Gaudin C, Belaich A, Champ S, Belaich JP (2000) CelE, a multidomain cellulase from Clostridium cellulolyticum: a key enzyme in the cellulosome? J Bacteriol 182:1910-1915
13. Han SO, Yukawa H, Inui M, Doi RH (2004) Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome. J Bacteriol 186:8347-8355
14. Henrissat B, Bairoch A (1996) Updating the sequence-based classification of glycosyl hydrolases. Biochem J 316:695-696
15. Irwin D, Shin DH, Zhang S, Barr BK, Sakon J, Karplus PA, Wilson DB (1998) Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis. J Bacteriol 180:1709-1714
16. Kataeva I, Li XL, Chen H, Choi SK, Ljungdahl LG (1999) Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination. J Bacteriol 181:5288-5295
17. Kosugi A, Murashima K, Tamaru Y, Doi RH (2002) Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation. Appl Environ Microbiol 68:6399-6402
18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
19. Ohmiya K, Sakka K, Karita S, Kimura T (1997) Structure of cellulases and their applications. Biotechnol Genet Eng Rev 14:365-414
20. Shoseyov O, Takagi M, Goldstein M, Doi, RH (1992) Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A (CbpA). Proc Natl Acad Sci USA 89:3483-3487
21. Sleat R, Mah RA, Robinson R (1984) Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium cellulovorans sp. nov. Appl Environ Microbiol 48:88-93
22. Tamaru Y, Doi RH (2001) Pectate lyase A, an enzymatic subunit of Clostridium cellulovorans cellulosome. Proc Natl Acad Sci U S A 98:4125-4129
23. Tamaru Y, Karita S, Ibrahim A, Chan H, Doi RH (2000) A large gene cluster for the Clostridium cellulovorans cellulosome. J Bacteriol 182:5906-5910
24. Tomme P, Warren RAJ, Gilkes NR (1995) Cellulose hydrolysis by bacteria and fungi. Adv Microb Physiol 37:1-81
25. Wang WK, Kruus K, Wu JHD (1993) Cloning and the DNA sequence of the gene coding for the Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component. J Bacteriol 175:1293-1302
26. Zverlov W, Schantz NN, Schwarz WH (2005) A major new component in the cellulosome of Clostridium thermocellum is a processive endo-beta-1,4-glucanase producing cellotetraose. FEMS Microbiol Lett 15:353-358