Amplified immunohistochemical detection of PrPsc in animal transmissible spongiform encephalopathies using streptomycin

Journal of Histochemistry and Cytochemistry

Volumn 54, Issue 8, 2006, Pages 849-853

  Bencsik, Anna A ^a   Coleman, Anthony W ^b   Debeer, Sabine O S ^a   Perron, Hervé ^c   Moussa, Aly ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b UNIVERSITÉ LYON 1   (France)

^c BIOMÉRIEUX   (France)

Author keywords

Amplification; Bovine spongiform encephalopathy; Diagnosis; Immunohistochemistry; Prion; Scrapie; Streptomycin

Indexed keywords

MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 2G11; MONOCLONAL ANTIBODY SAF84; PRION PROTEIN; STREPTOMYCIN SULFATE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; BOVINE SPONGIFORM ENCEPHALOPATHY; BRAIN SLICE; CONTROLLED STUDY; CREUTZFELDT JAKOB DISEASE; DEGENERATIVE DISEASE; IMMUNOHISTOCHEMISTRY; MOUSE; NERVE DEGENERATION; NONHUMAN; PRION DISEASE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SCRAPIE; SENSITIVITY ANALYSIS;

ANIMALS; BRAIN; CATTLE; ENCEPHALOPATHY, BOVINE SPONGIFORM; FEMALE; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; MICE; MICE, INBRED C57BL; PRPC PROTEINS; SCRAPIE; SHEEP; STREPTOMYCIN;

ANIMALIA; BOS TAURUS;

EID: 33746269413     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.5C6895.2006     Document Type: Article

References (12)

1. Andréoletti O, Berthon P, Marc D, Sarradin P, Grosclaude J, vanKeulen L, Scheicher F, et al. (2000) Early accumulation of PrPsc in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie. J Gen Virol 81:3115-3126
2. Bencsik A, Debeer S, Baron T (2005) An alternative pretreatment procedure in animal transmissible spongiform encephalopathies diagnosis using PrPsc immunohistochemistry. J Histochem Cytochem 53:1199-1202
3. Bolton DC, McKinley MP, Prusiner SB (1982) Identification of a protein that purifies with the scrapie prion. Science 218:1309-1311
4. Casalone C, Zanusso G, Acutis PL, Ferrari S, Capucci L, Tagliavini F, Monaco S, et al. (2004) Identification of a second bovine amyloidotic spongiform encephalopathy: molecular similarities with sporadic Creutzfeldt-Jakob disease. Proc Natl Acad Sci USA 101:3065-3070
5. Debeer S, Baron T, Bencsik A (2002) Transmissible spongiform encephalopathies diagnosis using PrPsc immunohistochemistry on fixed but previously frozen brain samples. J Histochem Cytochem 50:611-616
6. Debeer S, Baron T, Bencsik A (2003) Neuropathological characterisation of French bovine spongiform encephalopathy cases. Histochem Cell Biol 120:513-521
7. Jeffrey M, Martin S, Gonzalez L, Ryder SJ, Bellworthy SJ, Jackman R (2001) Differential diagnosis of infections with the bovine spongiform encephalopathy (BSE) and scrapie agents in sheep. J Comp Pathol 125:271-284
8. Kim SH, Shin YK, Lee KM, Lee JS, Yun JH, Lee SM (2003) An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining. J Histochem Cytochem 51:129-132
9. Köhler A, Lauritzen B, Van Noorden CJ (2000) Signal amplification in immunohistochemistry at the light microscopic level using biotinylated tyramide and nanogold-silver staining. J Histochem Cytochem 48:933-941
10. Montero C (2003) The antigen-antibody reaction in immunohistchemistry. J Histochem Cytochem 51:1-4
11. Moussa A, Coleman AW, Bencsik A, Leclere E, Perret F, Martin A, Perron H (2006) Use of streptomycin for precipitation and detection of proteinase K resistant prion protein (PrPsc) in biological samples. Chem Commun (Camb) 9:973-975
12. Shi SR, Cote RJ, Taylor CR (1997) Antigen retrieval immunohistochemistry: past, present, and future. J Histochem Cytochem 45:327-343