Retention of a differentiated endothelial phenotype by outgrowth endothelial cells isolated from human peripheral blood and expanded in long-term cultures

Cell and Tissue Research

Volumn 326, Issue 1, 2006, Pages 79-92

  Fuchs, Sabine ^a   Hermanns, Maria Iris ^a   Kirkpatrick, Charles James ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Endothelial progenitor cells; Human; Long term expansion cultures; Outgrowth endothelial cells; Peripheral blood; Phenotypic stability; Vascularization

Indexed keywords

CAVEOLIN 1; CD31 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; STEM CELL FACTOR RECEPTOR; VASCULAR ENDOTHELIAL CADHERIN; VASCULOTROPIN RECEPTOR 2;

ADOPTIVE IMMUNOTHERAPY; ANGIOGENESIS; ARTICLE; BLOOD; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL TYPE; ENDOTHELIUM CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL;

ANTIGENS, DIFFERENTIATION; CELLS, CULTURED; ENDOTHELIAL CELLS; HUMANS; ISCHEMIA; LEUKOCYTES, MONONUCLEAR; PHENOTYPE; REGENERATION; STEM CELL TRANSPLANTATION; STEM CELLS; TIME FACTORS; TISSUE THERAPY; TRANSPLANTATION, AUTOLOGOUS;

EID: 33746193153     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-006-0222-4     Document Type: Article

References (26)

1. Asahara T, Murohara T, Sullivan A, Silver M, Zee R van der, Li T, Witzenbichler B, Schatteman G, Isner JM (1997) Isolation of putative progenitor endothelial cells for angiogenesis. Science 275:964-966
2. Asahara T, Takahashi T, Masuda H, Kalka C, Chen D, Iwaguro H, Inai Y, Silver M, Isner JM (1999) VEGF contributes to postnatal neovascularization by mobilizing bone marrow-derived endothelial progenitor cells. EMBO J 18:3964-3972
3. Bardin N, Anfosso F, Masse J-M, Cramer E, Sabatier F, Bivic AL, Sampol J, Dignat-George F (2001) Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion. Blood 98:3677-3684
4. Boyer M, Townsend LE, Vogel LM, Falk J, Reitz-Vick D, Trevor KT, Villalba M, Bendick PJ, Glover JL (2000) Isolation of endothelial cells and their progenitor cells from human peripheral blood. J Vasc Surg 31:181-189
5. Dahlke MH, Larsen SR, Rasko JE, Schlitt HJ (2004) The biology of CD45 and its use as a therapeutic target. Leuk Lymphoma 45:229-236
6. Gehling UM, Ergun S, Schumacher U, Wagener C, Pantel K, Otte M, Schuch G, Schafhausen P, Mende T, Kilic N, Kluge K, Schafer B, Hossfeld DK, Fiedler W (2000) In vitro differentiation of endothelial cells from AC133-positive progenitor cells. Blood 95:3106-3112
7. Gill M, Dias S, Hattori K, Rivera ML, Hicklin D, Witte L, Girardi L, Yurt R, Himel H, Rafii S (2001) Vascular trauma induces rapid but transient mobilization of VEGFR2(+)AC133(+) endothelial precursor cells. Circ Res 88:167-174
8. Gulati R, Jevremovic D, Peterson TE, Chatterjee S, Shah V, Vile RG, Simari RD (2003) Diverse origin and function of cells with endothelial phenotype obtained from adult human blood. Circ Res 93:1023-1025
9. Hur J, Yoon CH, Kim HS, Choi JH, Kang HJ, Hwang KK, Oh BH, Lee MM, Park YB (2004) Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol 24:288-293
10. Ingram DA, Mead LE, Tanaka H, Meade V, Fenoglio A, Mortell K, Pollok K, FerkowiczMJ, Gilley D, YoderMC (2004) Identification of a novel hierarchy of endothelial progenitor cells utilizing human peripheral and umbilical cord blood. Blood 104:2752-2760
11. Jaffe EA, Nachman RL, Becker CG,Minick CR (1973) Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52:2745-2756
12. Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, Kearney M, Li T, Isner JM, Asahara T (2000) Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci USA 97:3422-3427
13. Klein CL, Kohler H, Bittinger F, Otto M, Hermanns I, Kirkpatrick CJ (1995) Comparative studies on vascular endothelium in vitro. 2. Hypoxia: its influences on endothelial cell proliferation and expression of cell adhesion molecules. Pathobiology 63:1-8
14. Krump-Konvalinkova V, Bittinger F, Unger RE, Peters K, Lehr HA, Kirkpatrick CJ (2001) Generation of human pulmonary microvascular endothelial cell lines. Lab Invest 81:1717-1727
15. Li T-S, Hamano K, Nishida M, Hayashi M, Ito H, Mikamo A, Matsuzaki M (2003) CD117+ stem cells play a key role in therapeutic angiogenesis induced by bone marrow cell implantation. Am J Physiol Heart Circ Physiol 285:H931-H937
16. Lin Y, Weisdorf DJ, Solovey A, Hebbel RP (2000) Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest 105:71-77
17. Liu J, Razani B, Tang S, Terman BI, Ware JA, Lisanti MP (1999) Angiogenesis activators and inhibitors differentially regulate caveolin-1 expression and caveolae formation in vascular endothelial cells. Angiogenesis inhibitors block vascular endothelial growth factor-induced down-regulation of caveolin-1. J Biol Chem 274:15781-15785
18. Liu J, Wang XB, Park DS, Lisanti MP (2002) Caveolin-1 expression enhances endothelial capillary tubule formation. J Biol Chem 277:10661-10668
19. Peichev M, Naiyer AJ, Pereira D, Zhu Z, Lane WJ, Williams M, Oz MC, Hicklin DJ, Witte L, Moore MA, Rafii S (2000) Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors. Blood 95:952-958
20. Pelosi E, Valtieri M, Coppola S, Botta R, Gabbianelli M, Lulli V, Marziali G, Masella B, Muller R, Sgadari C, Testa U, Bonanno G, Peschle C (2002) Identification of the hemangioblast in postnatal life. Blood 100:3203-3208
21. Salven P, Mustjoki S, Alitalo R, Alitalo K, Rafii S (2003) VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells. Blood 101:168-172
22. Shaw JP, Basch R, Shamamian P (2004) Hematopoietic stem cells and endothelial cell precursors express Tie-2, CD31 and CD45. Blood Cells Mol Diseases 32:168-175
23. Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, Ishida A, Fujita Y, Kothari S, Mohle R, Sauvage LR, Moore MA, Storb RF, Hammond WP (1998) Evidence for circulating bone marrow-derived endothelial cells. Blood 92:362-367
24. Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, Magner M, Isner JM, Asahara T (1999) Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med 5:434-438
25. Unger RE, Krump-Konvalinkova V, Peters K, Kirkpatrick CJ (2002) In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R. Microvasc Res 64:384-397
26. Yamamoto K, Takahashi T, Asahara T, Ohura N, Sokabe T, Kamiya A, Ando J (2003) Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress. J Appl Physiol 95:2081-2088