The use of flow cytometry to discriminate avian lymphocytes from contaminating thrombocytes

Developmental and Comparative Immunology

Volumn 30, Issue 9, 2006, Pages 843-850

  Bohls, Ryan L ^a   Smith, Roger ^a   Ferro, Pam J ^a   Silvy, Nova J ^b   Li, Zhongxia ^a   Collisson, Ellen W ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Avian lymphocytes; Dual labeling flow cytometry; Thrombocytes

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; B LYMPHOCYTE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL SUBPOPULATION; CONTAMINATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; LYMPHOCYTE; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; THROMBOCYTE;

AVES; TYMPANUCHUS;

EID: 33745969465     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2005.10.008     Document Type: Article

References (19)

1. Pei J., Briles W.E., and Collisson E.W. Memory T cells protect chicks from acute infectious bronchitis virus infection. Virology 306 2 (2003) 376-384
2. Seo S.H., et al. The carboxyl-terminal 120-residue polypeptide of infectious bronchitis virus nucleocapsid induces cytotoxic T lymphocytes and protects chickens from acute infection. J Virol 71 10 (1997) 7889-7894
3. Seo S.H., and Collisson E.W. Specific cytotoxic T lymphocytes are involved in in vivo clearance of infectious bronchitis virus. J Virol 71 7 (1997) 5173-5177
4. Seo S.H., et al. Adoptive transfer of infectious bronchitis virus primed alphabeta T cells bearing CD8 antigen protects chicks from acute infection. Virology 269 1 (2000) 183-189
5. Chan M.M., et al. Identification of the avian homologues of mammalian CD4 and CD8 antigens. J Immunol 140 7 (1988) 2133-2138
6. Chen C.L., et al. Identification of a T3/T cell receptor complex in chickens. J Exp Med 164 1 (1986) 375-380
7. Veromaa T., et al. Expression of B-L and Bu-1 antigens in chickens bursectomized at 60 h of incubation. Eur J Immunol 18 2 (1988) 225-230
8. Nicholson J.K., Jones B.M., and Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry 14 6 (1993) 685-689
9. Chung K.S., Lillehoj H.S., and Jenkins M.C. Avian leucocyte common antigens: molecular weight determination and flow cytometric analysis using new monoclonal antibodies. Vet Immunol Immunopathol 28 3-4 (1991) 259-273
10. Li Z., et al. Flow cytometric analysis of T lymphocyte subpopulations in large-bodied turkey lines and a randombred control population. Poult Sci 79 2 (2000) 219-223
11. Luhtala M., et al. Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes. Scand J Immunol 42 1 (1995) 171-174
12. Luhtala M. Chicken CD4, CD8alphabeta, and CD8alphaalpha T cell co-receptor molecules. Poult Sci 77 12 (1998) 1858-1873
13. Luhtala M., et al. A novel peripheral CD4+ CD8+ T cell population: inheritance of CD8 alpha expression on CD4+ Tcells. Eur J Immunol 27 1 (1997) 189-193
14. Luhtala M., et al. Analysis of chicken CD4 by monoclonal antibodies indicates evolutionary conservation between avian and mammalian species. Hybridoma 12 6 (1993) 633-646
15. Paramithiotis E., Tkalec L., and Ratcliffe M.J. High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes. J Immunol 147 11 (1991) 3710-3717
16. Kaspers B., Lillehoj H.S., and Lillehoj E.P. Chicken macrophages and thrombocytes share a common cell surface antigen defined by a monoclonal antibody. Vet Immunol Immunopathol 36 4 (1993) 333-346
17. Shevach E.M. Immunofluorescence and cell sorting. In: John A.M.K., Coligan E., Margulies D.H., Shevach E.M., and Strober W. (Eds). Current protocols in immunology (1992), New York, Wiley 5.3.6
18. Nicholson J., et al. Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping. Cytometry 26 3 (1996) 227-230
19. Traill K.N., Bock G., and Boyd R. Wick, Chicken thrombocytes, isolation, serological and functional characterization using fluorescence activated cell sorter. Dev Comp Immunol 7 (1983) 111-125