Microelectronic array system for molecular diagnostic genotyping: Nanogen NanoChip® 400 and Molecular Biology Workstation

Expert Review of Molecular Diagnostics

Volumn 6, Issue 3, 2006, Pages 287-294

  Keen Kim, Dianne ^a   Grody, Wayne W ^b,c   Richards, C Sue ^d,e  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Medical Genetics and Molecular Pathology   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d Molecular and Medical Genetics   (United States)

^e OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Analyte specific reagent; Microarray; Molecular biology workstation; NanoChip ; NanoChip 400; SNP genotyping

Indexed keywords

COST EFFECTIVENESS ANALYSIS; DNA MICROARRAY; GENOTYPE; MOLECULAR BIOLOGY; PUBLICATION; REVIEW; SINGLE NUCLEOTIDE POLYMORPHISM; BIOLOGY; DIAGNOSTIC PROCEDURE; HUMAN; INSTRUMENTATION; METHODOLOGY; MUTATION; NUCLEIC ACID HYBRIDIZATION; PHARMACOGENETICS;

COMPUTATIONAL BIOLOGY; GENOTYPE; HUMANS; MOLECULAR BIOLOGY; MOLECULAR DIAGNOSTIC TECHNIQUES; MUTATION; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PHARMACOGENETICS; POLYMORPHISM, SINGLE NUCLEOTIDE;

EID: 33745962246     PISSN: 14737159     EISSN: 17448352     Source Type: Journal    
DOI: 10.1586/14737159.6.3.287     Document Type: Review

References (15)

1. Heller MJ, Forster AH, Tu E. Active microelectronic chip devices which utilize controlled electrophoretic fields for multiplex DNA hybridization and other genomic applications. Electrophoresis 21, 157-164 (2000).
2. Su HJ, Surrey S, McKenzie SE, Fortina P, Graves DJ. Kinetics of heterogeneous hybridization on indium tin oxide surfaces with and without an applied potential. Electrophoresis 23(10), 1551-1557 (2002).
3. Thistlethwaite WA, Moses LM, Hoffbuhr KC, Devaney JM, Hoffman EP. Rapid genotyping of common MeCP2 mutations with an electronic DNA microchip using serial differential hybridization. J. Mol. Diagn. 5(2), 121-126 (2003). • Protocol for Rett syndrome testing on the Nanogen, Inc. instruments.
4. Foglieni B, Cremonesi L, Travi M et al. β-thalassemia microelectronic chip: a fast and accurate method for mutation detection. Clin. Chem. 50(1), 73-79 (2004). • Protocol for β-thalassemia testing on the Nanogen instruments.
5. Santacroce R, Ratti A, Caroli F et al. Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technology. Clin. Chem. 48(12), 2124-2130 (2002). • Protocol for Factor VII, β-globin and the RET proto-oncogene testing on the Nanogen instruments.
6. Erali M, Schmidt B, Lyon E, Wittwer C. Evaluation of electronic microarrays for genotyping factor V, factor II, and MTHFR. Clin. Chem. 49(5), 732-739 (2003). • Protocol for venous thrombosis testing on the Nanogen instruments.
7. Evans JG, Lee-Tataseo C. Determination of the factor V Leiden single-nucleotide polymorphism in a commercial clinical laboratory by use of NanoChip microelectronic array technology. Clin. Chem. 48(9), 1406-1411 (2002). • Protocol for Factor V testing on the Nanogen instruments.
8. Moutereau S, Narwa R, Matheron C, Vongmany N, Simon E, Goossens M. An improved electronic microarray-based diagnostic assay for identification of MEFV mutations. Hum. Mutat. 23(6), 621-628 (2004). • Protocol for familial Mediterranean fever testing on the Nanogen instruments.
9. Sohni YR, Dukek B, Taylor W, Ricart E, Sandborn WJ, O'Kane DJ. Active electronic arrays for genotyping of NAT2 polymorphisms. Clin. Chem. 47(10), 1922-1924 (2001). • Protocol for N-acetyltransferase testing on the Nanogen instruments.
10. Sethi AA, Tybjaerg-Hansen A, Andersen RV, Nordestgaard BG. Nanogen microelectronic chip for large-scale genotyping. Clin. Chem. 50(2), 443-446 (2004). • Protocol for β-adrenergic receptor and hepatic lipase gene testing on the Nanogen instruments.
11. Nagan N, O'Kane DJ. Validation of a single nucleotide polymorphism genotyping assay for the human serum paraoxonase gene using electronically active customized microarrays. Clin. Biochem. 34(7), 589-592 (2001). • Protocol for human serum paraoxonase testing on the Nanogen instruments.
12. Schutz E, von Ahsen N. Spreadsheet software for thermodynamic melting point prediction of oligonucleotide hybridization with and without mismatches. Biotechniques 27(6), 1218-1222, 1224 (1999).
13. Nanogen. NanoChip factor V Leiden research application note. In: NanoChip® Standard SNP Reseach Application Note. Document no. 140082.2, (2001).
14. Nanogen. Amplicon-down format with thermal discrimination. In: NanoChip® Standard SNP Reseach Application Note. Document no. 140083.1, (2001).
15. Dukek BA, O'Kane DJ. Seeking lower-cost genotype determinations. IVD Technol. 47 (2004). •• Reviews authors' use of Nanogen Molecular Biology Workstation.