Transient suppression of PPARγ directed ES cells into an osteoblastic lineage

FEBS Letters

Volumn 580, Issue 17, 2006, Pages 4121-4125

  Yamashita, Akihiro ^a   Takada, Tatsuyuki ^a   Nemoto, Ken ichi ^a   Yamamoto, Gaku ^a   Torii, Ryuzo ^a  

^a SHIGA UNIVERSITY OF MEDICAL SCIENCE   (Japan)

Author keywords

Adipogenesis; ES cell; Osteogenesis; Peroxisome proliferator activated receptor ; Small interfering RNA

Indexed keywords

CALCIUM; LIPID; MESSENGER RNA; OSTEOCALCIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; SMALL INTERFERING RNA; OSTEOPONTIN; SIALOGLYCOPROTEIN; SPP1 PROTEIN, MOUSE; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; MOUSE; MOUSE STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; ADIPOCYTE; ANIMAL; BIOSYNTHESIS; BONE MINERALIZATION; CELL LINE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; GENETICS; METABOLISM; PHYSIOLOGY; STEM CELL; UPREGULATION;

ADIPOCYTES; ANIMALS; CALCIFICATION, PHYSIOLOGIC; CALCIUM; CELL DIFFERENTIATION; CELL LINE; CELL LINEAGE; EMBRYO; MICE; OSTEOBLASTS; OSTEOPONTIN; PPAR GAMMA; RNA, MESSENGER; RNA, SMALL INTERFERING; SIALOGLYCOPROTEINS; STEM CELLS; TRANSCRIPTION FACTORS; UP-REGULATION;

EID: 33745839778     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.06.057     Document Type: Article

References (24)

1. Buttery L.D., Bourne S., Xynos J.D., Wood H., Hughes F.j., Hughes S.P., Episkopou V., and Polak J.M. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng. 7 (2001) 89-99
2. Phillips B.W., Belmonte N., Vernochet C., Ailhaud G., and Dani C. Compactin enhances osteogenesis in murine embryonic stem cells. Biochem. Biophys. Res. Commun. 284 (2001) 478-484
3. Sottile V., Thomson A., and Mcwhir J. In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 5 (2003) 149-155
4. Yamashita A., Takada T., Narita J., Yamamoto G., and Torii R. Osteoblastic differentiation of monkey embryonic stem cells in vitro. Cloning Stem Cells 7 (2005) 232-237
5. Nuttall M.E., Patton A.J., Olivera D.L., Nadeau D.P., and Gowen M. Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders. J. Bone Miner. Res. 13 (1998) 371-382
6. Beresford J.N., Bennett J.H., Devlin C., and Owen M.E. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J. Cell Sci. 102 (1992) 341-351
7. Meunier P., Aaron J., Edouard C., and Vignon G. Osteoporosis and the replacement of cell populations of the marrow by adipose tissue. A quantitative study of 84 iliac bone biopsies. Clin. Orthop. 80 (1971) 147-154
8. Rozman C., Feliu E., Berga L., Reverter J.C., Climent C., and Ferran M.J. Age-related variations of fat tissue fraction in normal human bone marrow depend both on size and number of adipocytes: a stereological study. Exp. Hematol. 17 (1989) 34-37
9. Kubota N., Terauchi Y., Miki H., Tamemoto H., Yamauchi T., Komeda K., Satoh S., Nakano R., Ishii C., Sugiyama T., Eto K., Tsubamoto Y., Okuno A., Murakami K., Sekihara H., Hasegawa G., Naito M., Toyoshima Y., Tanaka S., Shiota K., Kitamura T., Fujita T., Ezaki O., Aizawa S., Kadowaki T., et al. PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance. Mol. Cell 4 (1999) 597-609
10. Kadowaki T. Insights into insulin resistance and type 2 diabetes from knockout mouse models. J. Clin. Invest. 106 (2000) 459-465
11. Akune T., Ohba S., Kamekura S., Yamaguchi M., Chung U.I., Kubota N., Terauchi Y., Harada Y., Azuma Y., Nakamura K., Kadowaki T., and Kawaguchi H. PPARγ insufficiency enhances osteogenesis through osteoblast formation from bone marrow progenitors. J. Clin. Invest. 113 (2004) 846-855
12. Sharp P.A. RNA interference-2001. Genes Dev. 15 (2001) 485-490
13. Takada T., Nemoto K., Yamashita A., Kato M., Kondo Y., and Torii R. Efficient gene silencing and cell differentiation using siRNA in mouse and monkey ES cells. Biochem. Biophys. Res. Commun. 331 (2005) 1039-1044
14. Smith A.G. Culture and differentiation of embryonic stem cells. J. Tissue Culture Methods 13 (1991) 89-94
15. Dani C., Smith A.G., Dessolin S., Leroy P., Staccini L., Villageois P., Darimont C., and Ailhaud G. Differentiation of embryonic stem cells into adipocytes in vitro. J. Cell Sci. 110 (1997) 1279-1285
16. Elbashir S.M., Harborth J., Weber K., and Tuschl T. Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26 (2002) 199-213
17. Ramirez-Zacarias J.L., Castro-Munozledo F., and Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97 (1992) 493-497
18. McGee S.M. Histochemical methods for calcium. J. Histochem. 6 (1958) 2241
19. Yamamoto Y., Ohsaki Y., Goto T., Nakasima A., and Iijima T. Effects of static magnetic fields on bone formation in rat osteoblast cultures. J. Dent. Res. 82 (2003) 962-966
20. Gregoire F.M., Smas C.M., and Sul H.S. Understanding adipocyte differentiation. Physiol. Rev. 78 (1998) 783-809
21. Katayama K., Wada K., Miyoshi H., Ohashi K., Tachibana M., Furuki R., Mizuguchi H., Hayakawa T., Nakajima A., Kadowaki T., Tsutsumi Y., Nakagawa S., Kamisaki Y., and Mayumi T. RNA interfering approach for clarifying the PPARγ pathway using lentiviral vector expressing short hairpin RNA. FEBS Lett. 560 (2004) 178-182
22. Hosono T., Mizuguti H., Katayama K., Koizumi N., Kawabata K., Yamaguchi T., Nakagawa S., Watanabe Y., Mayumi T., and Hayakawa T. RNA interference of PPARγ using fiber-modified adenovirus vector efficiently suppresses preadipocyte-to-adipocyte differentiation in 3T3-L1 cells. Gene 348 (2005) 157-165
23. Yamashita A., Takada T., Kanbe-Omatsu M., Nemoto K., Matsuura H., Yamamoto G., and Torii R. Monkey embryonic stem cells differentiate into adipocytes in vitro. Cloning Stem Cells 8 (2006) 3-9
24. Zur Nieden N.I., Kempka G., and Ahr H.J. In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation 71 (2003) 18-27