Characterization of a second ligand binding site of the insulin receptor

Biochemical and Biophysical Research Communications

Volumn 347, Issue 1, 2006, Pages 334-339

  Hao, Caili ^a   Whittaker, Linda ^a   Whittaker, Jonathan ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

Author keywords

Affinity; Binding site; Fusion protein; Insulin; Insulin like growth factor I; Mutagenesis; Receptor

Indexed keywords

FIBRONECTIN; IMMUNOGLOBULIN FC FRAGMENT; INSULIN; INSULIN DEOCTAPEPTIDE; INSULIN RECEPTOR; PROINSULIN; RECEPTOR SUBTYPE; SOMATOMEDIN RECEPTOR;

ALPHA CHAIN; AMINO TERMINAL SEQUENCE; ARTICLE; BAY REGION BINDING SITE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; INSULIN BINDING; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING;

AMINO ACID SEQUENCE; BINDING SITES; CELLS, CULTURED; HUMANS; INSULIN; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RECEPTOR, INSULIN;

EID: 33745763872     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.06.089     Document Type: Article

References (30)

1. De Meyts P., and Whittaker J. Structural biology of insulin and IGF1 receptors: implications for drug design. Nat. Rev. Drug Discov. 1 (2002) 769-783
2. Scatchard G. The attractions of proteins for small molecules and ions. Ann. NY Acad. Sci. 52 (1949) 660-672
3. De Meyts P., Roth J., Neville D.M., Gavin J.R., and Lesniak M.A. Insulin interactions with its receptors: experimental evidence for negative cooperativity. Biochem. Biophys. Res. Commun. 55 (1973) 154-161
4. Markussen J., Halstrom J., Wiberg F.C., and Schaffer L. Immobilized insulin for high capacity affinity chromatography of insulin receptors. J. Biol. Chem. 266 (1991) 18814-18818
5. De Meyts P. The structural basis of insulin and insulin-like growth factor-I receptor binding and negative co-operativity, and its relevance to mitogenic versus metabolic signalling. Diabetologia 37 (1994) S135-S148
6. Hammond B.J., Tikerpae J., and Smith G.D. An evaluation of the cross-linking model for the interaction of insulin with its receptor. Am. J. Physiol. 272 (1997) E1136-E1144
7. Lee J., and Pilch P.F. The insulin receptor: structure, function, and signaling. Am. J. Physiol. 266 (1994) C319-C334
8. Schaffer L. A model for insulin binding to the insulin receptor. Eur. J. Biochem. 221 (1994) 1127-1132
9. Kristensen C., Andersen A.S., Ostergaard S., Hansen P.H., and Brandt J. Functional reconstitution of insulin receptor binding site from non-binding receptor fragments. J. Biol. Chem. 277 (2002) 18340-18345
10. Whittaker J., Sorensen H., Gadsboll V.L., Hinrichsen J., and Whittaker L.J. Comparison of the functional insulin binding epitopes of the A and B isoforms of the insulin receptor. J. Biol. Chem. 277 (2002) 47380-47384
11. Schumacher R., Soos M.A., Schlessinger J., Brandenburg D., Siddle K., and Ullrich A. Signaling-competent receptor chimeras allow mapping of major insulin receptor binding domain determinants. J. Biol. Chem. 268 (1993) 1087-1094
12. Zhang B., and Roth R.A. A region of the insulin receptor important for ligand binding (residues 450-601) is recognized by patients' autoimmune antibodies and inhibitory monoclonal antibodies. Proc. Natl. Acad. Sci. USA 88 (1991) 9858-9862
13. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
14. Schaffer L., Larsen U.D., Linde S., Hejnaes K.R., and Skriver L. Characterization of the three 125I-iodination isomers of human insulin-like growth factor I (IGF1). Biochim. Biophys. Acta 1203 (1993) 205-209
15. Kirsch R.D., and Joly E. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids Res. 26 (1998) 1848-1850
16. Mynarcik D.C., Williams P.F., Schaffer L., Yu G.Q., and Whittaker J. Identification of common ligand binding determinants of the insulin and insulin-like growth factor 1 receptors. Insights into mechanisms of ligand binding. J. Biol. Chem. 272 (1997) 18650-18655
17. Whittaker J., Okamoto A.K., Thys R., Hofmann C.A., Bell G.I., and Steiner D.F. High-level expression of human insulin receptor cDNA in mouse NIH 3T3 cells. Proc. Natl. Acad. Sci. USA 84 (1987) 5237-5241
18. Clackson T., and Winter G. 'Sticky feet'-directed mutagenesis and its application to swapping antibody domains. Nucleic Acids Res. 17 (1989) 10163-10170
19. Christoffersen C.T., Bornfeldt K.E., Rotella C.M., Gonzales N., Vissing H., Shymko M., Ten Hoeve J., Groffen J., Heisterkamp N., De Meyts P., Wood S., and Vogt P.H. Negative cooperativity in the insulin-like growth factor-I receptor and a chimeric IGF-I/insulin receptor. Endocrinology 135 (1994) 472-475
20. Wang Z.X. An exact mathematical expression for describing competitive binding of two different ligands to a protein molecule. FEBS Lett. 360 (1995) 111-114
21. Harlow E., and Lane D. Using Antibodies: A Laboratory Manual (1999), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
22. Mynarcik D.C., Yu G.Q., and Whittaker J. Alanine-scanning mutagenesis of a C-terminal ligand binding domain of the insulin receptor alpha subunit. J. Biol. Chem. 271 (1996) 2439-2442
23. Williams P.F., Mynarcik D.C., Yu G.Q., and Whittaker J. Mapping of an NH2-terminal ligand binding site of the insulin receptor by alanine scanning mutagenesis. J. Biol. Chem. 270 (1995) 3012-3016
24. Brandt J., Andersen A.S., and Kristensen C. Dimeric fragment of the insulin receptor alpha-subunit binds insulin with full holoreceptor affinity. J. Biol. Chem. 276 (2001) 12378-12384
25. Surinya K.H., Molina L., Soos M.A., Brandt J., Kristensen C., and Siddle K. Role of insulin receptor dimerization domains in ligand binding, cooperativity, and modulation by anti-receptor antibodies. J. Biol. Chem. 277 (2002) 16718-16725
26. Kjeldsen T., Andersen A.S., Wiberg F.C., Rasmussen J.S., Schaffer L., Balschmidt P., Moller K.B., and Moller N.P. The ligand specificities of the insulin receptor and the insulin-like growth factor I receptor reside in different regions of a common binding site. Proc. Natl. Acad. Sci. USA 88 (1991) 4404-4408
27. Bennett M.J., Choe S., and Eisenberg D. Domain swapping: entangling alliances between proteins. Proc. Natl. Acad. Sci. USA 91 (1994) 3127-3131
28. Baker E.N., Blundell T.L., Cutfield J.F., Cutfield S.M., Dodson E.J., Dodson G.G., Hodgkin D.M., Hubbard R.E., Isaacs N.W., and Reynolds C.D. The structure of 2Zn pig insulin crystals at 1.5 Å resolution. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 319 (1988) 369-456
29. De Meyts P. Insulin and its receptor: structure, function and evolution. Bioessays 26 (2004) 1351-1362
30. Wells J.A. Additivity of mutational effects in proteins. Biochemistry 29 (1990) 8509-8517