Polyethylenimine, a cost-effective transfection reagent

Signal Transduction

Volumn 6, Issue 3, 2006, Pages 179-184

  Ehrhardt, Christina ^a,b   Schmolke, Mirco ^a   Matzke, Andreas ^a   Knoblauch, Alexander ^a   Will, Carola ^a   Wixler, Viktor ^a   Ludwig, Stephan ^a  

^a ZMBE   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Human embryonic kidney (HEK 293) cells; Madin Darby canine kidney (MDCK) cells; Polyethylenimine (PEI); Transient transfection

Indexed keywords

PLASMID DNA; POLYETHYLENEIMINE; REAGENT;

ANIMAL CELL; ARTICLE; CELL LINE; CELL STRAIN; CELL STRAIN 3T3; CELL STRAIN HEK293; CHO CELL; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; EMBRYO CELL; EPITHELIUM CELL; FIBROBLAST; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; KIDNEY CELL; KIDNEY EPITHELIUM; NONHUMAN; OVARY CELL; PRIORITY JOURNAL;

EID: 33745742878     PISSN: 16154053     EISSN: 16154061     Source Type: Journal    
DOI: 10.1002/sita.200500073     Document Type: Article

References (6)

1. Boussif, O., Lezoualc'h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B., Behr, J.P. (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. U.S.A. 92: 7297-301.
2. Sonawane, N.D., Szoka, F.C. Jr., Verkman, A.S. (2003) Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes. J. Biol. Chem. 278: 44826-44831.
3. Durocher, Y., Perret, S., Kamen, A. (2002) High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res. 30: E9.
4. Ehrhardt, C., Kardinal, C., Wurzer, W.J., Wolff, T., von Eichel-Streiber, C., Pleschka, S., Planz, O., Ludwig, S. (2004) Rac1 and PAK1 are upstream of IKK-epsilon and TBK-1 in the viral activation of interferon regulatory factor-3. FEBS Lett. 567: 230-8.
5. Basler, C.F., Wang, X., Muhlberger, E., Volchkov, V., Paragas, J., Klenk, H.D., Garcia-Sastre, A., Palese, P. (2000) The Ebola virus VP35 protein functions as a type I IFN antagonist. Proc Natl Acad Sci U S A 97: 12289-94.
6. Wurzer, W.J., Ehrhardt, C., Pleschka, S., Berberich-Siebelt, F., Wolff, T., Walczak, H., Planz, O., Ludwig, S. (2004) NF-kappaB-dependent induction of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas/FasL is crucial for efficient influenza virus propagation. J. Biol. Chem. 279: 30931-30937.