Highly efficient ribosome display selection by use of purified components for in vitro translation

Journal of Immunological Methods

Volumn 313, Issue 1-2, 2006, Pages 140-148

  Villemagne, Denis ^a   Jackson, Ronald ^a   Douthwaite, Julie A ^a  

^a MEDIMMUNE LTD   (United Kingdom)

Author keywords

Cell free translation; PUREsystem; Real time PCR; Reconstituted translation; Ribosome display

Indexed keywords

COMPLEMENTARY DNA; ESCHERICHIA COLI PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; GENE LIBRARY; GENOTYPE; IN VITRO STUDY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PROTEIN STABILITY; PURIFICATION; RIBOSOME; RNA TRANSLATION;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMAN GROWTH HORMONE; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; INSULIN; INTERLEUKIN-13; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN BIOSYNTHESIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBOSOMES; RNA STABILITY;

ESCHERICHIA COLI; PROKARYOTA;

EID: 33745727452     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.04.001     Document Type: Article

References (14)

1. Forster A.C., Cornish C.W., and Blacklow S.C. Pure translation display. Anal. Biochem. 333 (2004) 358
2. Hanes J., Jermutus L., Weber-Bornhauser S., Bosshard H.R., and Plückthun A. Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries. Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 14130
3. Hanes J., Jermutus L., and Plückthun A. Selecting and evolving functional proteins in vitro by ribosome display. In: Abelson J.N., and Simon M.I. (Eds). Methods Enzymol. (2000), Academic Press, San Diego 404
4. Hanes J., Schaffitzel C., Knappik A., and Plückthun A. Picomolar affinity antibodies from a fully synthetic naïve library selected and evolved by ribosome display. Nat. Biotechnol. 18 (2000) 1287
5. Hutchings C., Carmen S., and Lennard S. Generation of Naïve Human Antibody Libraries. In: Kontermann R., and Dubel S. (Eds). Antibody Engineering. Springer Laboratory Manuals (2001) 93
6. Jermutus L., Ryabova L.A., and Plückthun A. Recent advances in producing and selecting functional proteins by using cell-free translation. Curr. Opin. Biotechnol. 9 (1998) 534
7. Jermutus L., Honegger A., Schwesinger F., Hanes J., and Plückthun A. Tailoring in vitro evolution for protein affinity or stability. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 75
8. Katzen F., Chang G., and Kudlicki W. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23 (2005) 150
9. Mattheakis L.C., Bhatt R.R., and Dower W.J. An in vitro polysome display system for identifying ligands from very large peptide libraries. Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 9022
10. McCafferty J., Fitzgerald K.J., Earnshaw J., Chiswell D.J., Link J., Smith R., and Kenten J. Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display. Appl. Biochem. Biotechnol. 47 (1994) 157
11. Shimizu Y., Inoue A., Tomari Y., Suzuki T., Yokogawa T., Nishikawa K., and Ueda T. Cell-free translation reconstituted with purified components. Nat. Biotechnol. 19 (2001) 751
12. Shimizu Y., Kanamori T., and Ueda T. Protein synthesis by pure translations systems. Methods 36 (2005) 299
13. Ying B.-W., Taguchi H., Ueda H., and Ueda T. Chaperone-assisted folding of a single-chain antibody in a reconstituted system. Biochem. Biophys. Res. Commun. 320 (2004) 1359
14. Zubay G. In vitro synthesis of protein in microbial systems. Annu. Rev. Genet. 7 (1973) 267