An improved method for RNA isolation and removal of melanin contamination from melanoma tissue: Implications for tumor antigen detection and amplification

Journal of Immunological Methods

Volumn 313, Issue 1-2, 2006, Pages 119-128

  Dörrie, Jan ^a   Wellner, Verena ^a   Kämpgen, Eckhart ^a   Schuler, Gerold ^a   Schaft, Niels ^a  

^a UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

Antigen detection amplification; Melanin; RNA DNA; RT PCR

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCOPROTEIN GP 100; MELANIN; MONOPHENOL MONOOXYGENASE; POLYNUCLEOTIDE; RNA; SILICON DIOXIDE; TUMOR ANTIGEN;

ABSORPTION; ANTIGEN DETECTION; ARTICLE; CANCER TISSUE; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTAMINATION; CONTROLLED STUDY; CORRELATION ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; ION EXCHANGE CHROMATOGRAPHY; MELANOMA; PHOTOMETRY; PIGMENTATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA BINDING; RNA ISOLATION; RNA PURIFICATION;

ANTIGENS, NEOPLASM; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, AGAR GEL; HUMANS; MELANINS; MELANOMA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, NEOPLASM; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 33745685149     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.04.003     Document Type: Article

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