Detection of anti-EPO antibodies in human sera by a bridging ELISA is much more sensitive when coating biotinylated rhEPO to streptavidin rather than using direct coating of rhEPO

Journal of Immunological Methods

Volumn 313, Issue 1-2, 2006, Pages 176-182

  Gross, Johann ^a   Moller, Renate ^a   Henke, Wolfgang ^a   Hoesel, Wolfgang ^b  

^a HUMBOLDT UNIVERSITY   (Germany)

^b ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Anti erythropoietin antibody ELISA; Direct coating; Recombinant human erythropoietin (rhEPO); Streptavidin biotin coupling

Indexed keywords

EPITOPE; ERYTHROPOIETIN ANTIBODY; RECOMBINANT ERYTHROPOIETIN; STREPTAVIDIN;

ANTIBODY DETECTION; ANTIGEN BINDING; ARTICLE; BIOTINYLATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; INTERMETHOD COMPARISON; MICROTITER PLATE ASSAY; PRIORITY JOURNAL; SCREENING TEST; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SERUM;

ANTIBODIES; BINDING, COMPETITIVE; BIOTINYLATION; CALIBRATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROPOIETIN; ERYTHROPOIETIN, RECOMBINANT; HUMANS; REPRODUCIBILITY OF RESULTS; SERUM; STREPTAVIDIN;

EID: 33745685141     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.04.008     Document Type: Article

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