Preparation and properties of thermoresponsive bioconjugates of trypsin

Artificial Cells, Blood Substitutes, and Biotechnology

Volumn 34, Issue 3, 2006, Pages 323-336

  Raghava, Smita ^a   Mondal, Kalyani ^a   Gupta, Munishwar ^a   Pareek, Pradeep ^b   Kuckling, Dirk ^b  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Enzyme immobilization; Poly(N isopropylacrylamide); Smart bioconjugates; Smart polymers; Trypsin

Indexed keywords

BIOMATERIALS; CARBOXYLIC ACIDS; ORGANIC POLYMERS; STABILITY;

POLY(N- ISOPROPYLACRYLAMIDE; SMART BIOCONJUGATES; SMART POLYMERS; TRYPSIN;

ENZYME IMMOBILIZATION;

6 ACRYLAMINOHEXANOIC ACID; CYANAMIDE; N HYDROXYSUCCINIMIDE; POLY(N ISOPROPYLACRYLAMIDE); SUCCINIMIDE DERIVATIVE; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; AUTOLYSIS; CONJUGATION; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME STABILITY; HEAT SENSITIVITY; PROTEIN BINDING; SOLUBILITY; THERMAL ANALYSIS;

ACRYLIC RESINS; ANIMALS; AUTOLYSIS; BIOPOLYMERS; CATTLE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; MATERIALS TESTING; SPECTROMETRY, FLUORESCENCE; TRYPSIN; TRYPSIN INHIBITORS;

EID: 33745685049     PISSN: 10731199     EISSN: 15324184     Source Type: Journal    
DOI: 10.1080/10731190600683902     Document Type: Article

References (20)

1. Guisán, J.M., Bastida, A., Cuesta, A.C., Fernández- Lafuente, R., Rosell, C.M. (1991). Immobilization-stabilization of chymotrypsin by covalent attachment to aldehyde agarose gels. Biotechnol. Bioeng. 39: 75-84.
2. Adlercreutz, P. (1993). Immobilized enzymes, in Enzymes in Food Processing, G. Reed, Ed., Academic Press: New York, pp. 103-119.
3. Saleemuddin, M. (1999). Bioaffinity based immobilization of enzymes. Adv. Biochem. Eng. Biotechnol. 64: 203-226.
4. Roy, I., Sharma, S., Gupta, M.N. (2003). Smart biocatalysts: Design and applications. Adv. Biochem. Eng/Biotechnol. 86: 159-189.
5. Fujii, M., Taniguchi, M. (1991). Application of reversibly soluble-insoluble polymers in bioprocessing. Trends. Biotechnol. 9: 191-196.
6. Shiroya, T., Yasui, M., Fujimoto, K., Kawaguchi, H. (1995). Control of enzymatic activity using thermoresponsive polymers. Colloids Surf. B: Biointerfaces. 4: 275-285.
7. Chen, J.-P., Hsu, M.-S. (1997). Preparations and properties of temperature-sensitive poly(N-isopropylacrylamide)-chymotrypsin conjugates. J. Mol. Catal. B Enzymatic. 2: 233-241.
8. Ding, Z., Chen, G., Hoffman, A.S. (1998). Unusual properties of thermally sensitive oligomer-enzyme conjugates of poly (N-isopropylacrylamide)-trypsin. J. Biomed. Mater. Res. 39: 498-505.
9. Ivanov, A.E., Edink, E., Kumar, A., Galaev, I.Y., Arendsen, A.F., Bruggink, A., Mattiasson, B. (2003). Conjugation of penicillin acylase with the reactive copolymer of N-Isopropylacrylamide: A step toward a thermoresponsive industrial biocatalyst. Biotechnol. Prog. 19: 1167-1175.
10. Kuckling, D., Adler, H.-J.P., Arndt, K.-F., Ling, L., Habicher, W.D. (2000). Temperature and pH dependent solubility of novel poly(N- isopropylacrylamide) copolymers. Macromol. Chem. Phys. 201: 273-280.
11. Erlanger, B.F., Kokowsky, N., Cohen, W. (1961). The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95: 271-278.
12. Fujimora, M., Mori, T., Tosa, T. (1987). Preparation and properties of soluble-insoluble immobilized proteases. Biotechnol. Bioeng. 29: 747-752.
13. Lowry, O.H., Rosebrough, N.J., Fall, A.C., Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
14. Decker, L.A., Ed. (1977). Worthington Enzyme Manual, Worthington Biochemical Corporation: Freehold, New Jersey, pp. 221-224.
15. Hermanson, G.T., Mallia, A.K., Smith, P.K., Ed. (1992). Activation methods, in Immobilized Affinity Ligand Techniques, Academic Press: San Diego, pp. 53-95.
16. Cornish-Bowden, A., Ed. (1995). Analysis of Enzyme Kinetic Data, Oxford University Press: Oxford, pp. 27-36.
17. Arasaratnam, V., Galaev I.Y., Mattiasson, B. (2000). Reversibly soluble biocatalyst: Optimisation of trypsin coupling to Eudragit S-100 and biocatalyst activity in soluble and precipitated forms. Enzyme Microb. Technol. 27: 254-263.
18. Palmieri, G., Giardina, P., Desiderio, B., Marzullo, L., Giamberini, M., Sannia, G. (1994). A new enzyme immobilization procedure using copper alginate gel: application to a fungal phenol oxidase. Enzyme Microb. Technol. 16: 151-158.
19. Markvicheva, E.A., Tkachuk, N.E., Kuptsova, S.V., Dugina, T.N., Strukova, S.M., Kirsh, Yu.E., Zubov, V.P., Rumsh, L.D. (1996). Stabilization of proteases by entrapment in a new composite hydrogel. Appl. Biochem. Biotechnol. 61: 75-84.
20. Jain, S., Roy, I., Gupta, M.N. (2004). A smart bioconjugate of trypsin with alginate. Artif. Cells Blood Substit. Biotechnol. 32: 325-337.