Activation and enzymatic characterization of recombinant human kallikrein 8

Biological Chemistry

Volumn 387, Issue 6, 2006, Pages 723-731

  Kishi, Tadaaki ^a,b,c   Cloutier, Sylvain M ^c,d   Kündig, Christoph ^c,d   Deperthes, David ^c,d   Diamandis, Eleftherios P ^a,b  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^d Medical Discovery SA   (Switzerland)

Author keywords

Cancer biomarkers; Human kallikrein 8; Human kallikreins; k cat Km; Lysyl endopeptidase; Serine protease

Indexed keywords

ANTIPAIN; ARGININE; CALCIUM ION; CHYMOSTATIN; COUMARIN DERIVATIVE; LEUPEPTIN; LYSINE; MAGNESIUM ION; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; NEUROPSIN; PHOSPHORAMIDON; POTASSIUM ION; PROTEINASE INHIBITOR; SODIUM ION; TERT BUTOXYCARBONYLVALYLPROLYLARGINYL 7 AMIDO 4 METHYLCOUMARIN; TOSYLLYSYL CHLOROMETHYL KETONE; UNCLASSIFIED DRUG; ZINC ION;

ALZHEIMER DISEASE; CONCENTRATION RESPONSE; CONFERENCE PAPER; DISEASE ASSOCIATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; HUMAN; INHIBITION KINETICS; KALLIKREIN KININ SYSTEM; METAL BINDING; OPTIMAL DRUG DOSE; OVARY CANCER; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; CALCIUM; COUMARINS; ENZYME ACTIVATION; HUMANS; HYDROGEN-ION CONCENTRATION; KALLIKREINS; METALS, HEAVY; OLIGOPEPTIDES; PROTEASE INHIBITORS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 33745679371     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2006.091     Document Type: Conference Paper

References (61)

1. Ahsan, M.N. and Watabe, S. (2001). Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus. J. Protein Chem. 20, 49-58.
2. Akita, H., Matsuyama, T., Iso, H., Sugita, M., and Yoshida, S. (1997). Effects of oxidative stress on the expression of limbic-specific protease neuropsin and avoidance learning in mice. Brain Res. 769, 86-96.
3. Basu, A., Dillon, R.D., Taylor, R., Davison, J.M., and Marshall, S.M. (2004). Is normalisation of serum potassium and magnesium always necessary in Gitelman syndrome for a successful obstetric outcome? BJOG 111, 630-634.
4. Bernett, M.J., Blaber, S.I., Scarisbrick, I.A., Dhanarajan, P., Thompson, S.M., and Blaber, M. (2002). Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system. J. Biol. Chem. 277, 24562-24570.
5. Bode, W. and Schwager, P. (1975). The single calcium-binding site of crystalline bovine β-trypsin. FEBS Lett. 56, 139-143.
6. Borgono, C.A. and Diamandis, E.P. (2004). The emerging roles of human tissue kallikreins in cancer. Nat. Rev. Cancer 4, 876-890.
7. Borgono, C.A., Grass, L., Soosaipillai, A., Yousef, G.M., Petraki, C.D., Howarth, D.H., Fracchioli, S., Katsaros, D., and Diamandis, E.P. (2003). Human kallikrein 14: a new potential biomarker for ovarian and breast cancer. Cancer Res. 63, 9032-9041.
8. Brattsand, M., Stefansson, K., Lundh, C., Haasum, Y., and Egelrud, T. (2005). A proteolytic cascade of kallikreins in the stratum corneum. J. Invest. Dermatol. 124, 198-203.
9. Campbell, R.C. (1989). Statistics for Biologists, 3rd ed. (Cambridge, UK: Cambridge University Press).
10. Cane, S., Bignotti, E., Bellone, S., Palmieri, M., De las Casas, L., Roman, J.J., Pecorelli, S., Cannon, M.J., O'Brien, T., and Santin, A.D. (2004). The novel serine protease tumor-associated differentially expressed gene-14 (KLK8/Neuropsin/Ovasin) is highly overexpressed in cervical cancer. Am. J. Obstet. Gynecol. 190, 60-66.
11. Chen, Z.L., Yoshida, S., Kato, K., Momota, Y., Suzuki, J., Tanaka, T., Ito, J., Nishino, H., Aimoto, S., Kiyama, H., et al. (1995). Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus. J. Neurosci. 15, 5088-5097.
12. Cloutier, S.M., Chagas, J.R., Mach, J.P., Gygi, C.M., Leisinger, H.J., and Deperthes, D. (2002). Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology. Eur. J. Biochem. 269, 2747-2754.
13. Cloutier, S.M., Kundig, C., Felber, L.M., Fattah, O.M., Chagas, J.R., Gygi, C.M., Jichlinski, P., Leisinger, H.J., and Deperthes, D. (2004). Development of recombinant inhibitors specific to human kallikrein 2 using phage-display selected substrates. Eur. J. Biochem. 271, 607-613.
14. Davies, B.J., Pickard, B.S., Steel, M., Morris, R.G., and Lathe, R. (1998). Serine proteases in rodent hippocampus. J. Biol. Chem. 273, 23004-23011.
15. Deperthes, D., Chapdelaine, P., Tremblay, R.R., Brunet, C., Berton, J., Hebert, J., Lazure, C., and Dube, J.Y. (1995). Isolation of prostatic kallikrein hK2, also known as hGK-1, in human seminal plasma. Biochim. Biophys. Acta 1245, 311-316.
16. Diamandis, E.P., Yousef, G.M., Clements, J., Ashworth, L.K., Yoshida, S., Egelrud, T., Nelson, P.S., Shiosaka, S., Little, S., Lilja, H., et al. (2000a). New nomenclature for the human tissue kallikrein gene family. Clin. Chem. 46, 1855-1858.
17. Diamandis, E.P., Yousef, G.M., Soosaipillai, A.R., Grass, L., Porter, A., Little, S., and Sotiropoulou, G. (2000b). Immunofluorometric assay of human kallikrein 6 (zyme/protease M/neurosin) and preliminary clinical applications. Clin. Biochem. 33, 369-375.
18. Diamandis, E.P., Okui, A., Mitsui, S., Luo, L.Y., Soosaipillai, A., Grass, L., Nakamura, T., Howarth, D.J., and Yamaguchi, N. (2002). Human kallikrein 11: a new biomarker of prostate and ovarian carcinoma. Cancer Res. 62, 295-300.
19. Egelrud, T. (1993). Purification and preliminary characterization of stratum corneum chymotryptic enzyme: a proteinase that may be involved in desquamation. J. Invest. Dermatol. 101, 200-204.
20. Elmoujahed, A., Gutman, N., Brillard, M., and Gauthier, F. (1990). Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland. FEBS Lett. 265, 137-140.
21. Felber, L.M., Borgono, C.A., Cloutier, S.M., Kundig, C., Kishi, T., Ribeiro Chagas, J., Jichlinski, P., Gygi, C.M., Leisinger, H.J., Diamandis, E.P., and Deperthes, D. (2005). Enzymatic profiling of human kallikrein 14 using phage-display substrate technology. Biol. Chem. 386, 291-298.
22. Fiedler, F. and Leysath, G. (1979). Substrate specificity of porcine pancreatic kallikrein. Adv. Exp. Med. Biol. 120A, 261-271.
23. Franzke, C.W., Baici, A., Bartels, J., Christophers, E., and Wiedow, O. (1996). Antileukoprotease inhibits stratum corneum chymotryptic enzyme. Evidence for a regulative function in desquamation. J. Biol. Chem. 271, 21886-21890.
24. Gomis-Ruth, F.X., Bayes, A., Sotiropoulou, G., Pampalakis, G., Tsetsenis, T., Villegas, V., Aviles, F.X., and Coll, M. (2002). The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family. J. Biol. Chem. 277, 27273-27281.
25. Hansson, L., Stromqvist, M., Backman, A., Wallbrandt, P., Carlstein, A., and Egelrud, T. (1994). Cloning, expression, and characterization of stratum corneum chymotryptic enzyme. A skin-specific human serine proteinase. J. Biol. Chem. 269, 19420-19426.
26. Huntington, J.A., Read, R.J., and Carrell, R.W. (2000). Structure of a serpin-protease complex shows inhibition by deformation. Nature 407, 923-926.
27. Jeohn, G.H., Serizawa, S., Iwamatsu, A., and Takahashi, K. (1995). Isolation and characterization of gastric trypsin from the microsomal fraction of porcine gastric antral mucosa. J. Biol. Chem. 270, 14748-14755.
28. Kapadia, C., Chang, A., Sotiropoulou, G., Yousef, G.M., Grass, L., Soosaipillai, A., Xing, X., Howarth, D.H., and Diamandis, E.P. (2003). Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensitive and specific immunofluorometric assay. Clin. Chem. 49, 77-86.
29. Kapadia, C., Yousef, G.M., Mellati, A.A., Magklara, A., Wasney, G.A., and Diamandis, E.P. (2004). Complex formation between human kallikrein 13 and serum protease inhibitors. Clin. Chim. Acta 339, 157-167.
30. Kishi, T., Kato, M., Shimizu, T., Kato, K., Matsumoto, K., Yoshida, S., Shiosaka, S., and Hakoshima, T. (1999). Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis. J. Biol. Chem. 274, 4220-4224.
31. Kishi, T., Grass, L., Soosaipillai, A., Scorilas, A., Harbeck, N., Schmalfeldt, B., Dorn, J., Mysliwiec, M., Schmitt, M., and Diamandis, E.P. (2003a). Human kallikrein 8, a novel biomarker for ovarian carcinoma. Cancer Res. 63, 2771-2774.
32. Kishi, T., Grass, L., Soosaipillai, A., Shimizu-Okabe, C., and Diamandis, E.P. (2003b). Human kallikrein 8: immunoassay development and identification in tissue extracts and biological fluids. Clin. Chem. 49, 87-96.
33. Kishi, T., Soosaipillai, A., Grass, L., Little, S.P., Johnstone, E.M., and Diamandis, E.P. (2004). Development of an immunofluorometric assay and quantification of human kallikrein 7 in tissue extracts and biological fluids. Clin. Chem. 50, 709-716.
34. Laxmikanthan, G., Blaber, S.I., Bernett, M.J., Scarisbrick, I.A., Juliano, M.A., and Blaber, M. (2005). 1.70 Å X-ray structure of human apo kallikrein 1: structural changes upon peptide inhibitor/substrate binding. Proteins 58, 802-814.
35. 2+ and extracellular protease inhibitors. Eur. J. Biochem. 262, 781-789.
36. Luo, L.Y., Grass, L., Howarth, D.J., Thibault, P., Ong, H., and Diamandis, E.P. (2001). Immunofluorometric assay of human kallikrein 10 and its identification in biological fluids and tissues. Clin. Chem. 47, 237-246.
37. Luo, L.Y., Shan, S.J., Elliott, M.B., Soosaipillai, A., and Diamandis, E.P. (2006). Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma. Clin. Cancer Res. 12, 742-750.
38. Maeda, S. (1989). Expression of foreign genes in insects using baculovirus vectors. Annu. Rev. Entomol. 34, 351-372.
39. Magklara, A., Mellati, A.A., Wasney, G.A., Little, S.P., Sotiropoulou, G., Becker, G.W., and Diamandis, E.P. (2003). Characterization of the enzymatic activity of human kallikrein 6: autoactivation, substrate specificity, and regulation by inhibitors. Biochem. Biophys. Res. Commun. 307, 948-955.
40. Michael, I.P., Sotiropoulou, G., Pampalakis, G., Magklara, A., Ghosh, M., Wasney, G., and Diamandis, E.P. (2005). Biochemical and enzymatic characterization of human kallikrein 5 (hK5), a novel serine protease potentially involved in cancer progression. J. Biol. Chem. 280, 14628-14635.
41. Momota, Y., Yoshida, S., Ito, J., Shibata, M., Kato, K., Sakurai, K., Matsumoto, K., and Shiosaka, S. (1998). Blockade of neuropsin, a serine protease, ameliorates kindling epilepsy. Eur. J. Neurosci. 10, 760-764.
42. Obiezu, C.V., Shan, S.J., Soosaipillai, A., Luo, L.Y., Grass, L., Sotiropoulou, G., Petraki, C.D., Papanastasiou, P.A., Levesque, M.A., and Diamandis, E.P. (2005). Human kallikrein 4: quantitative study in tissues and evidence for its secretion into biological fluids. Clin. Chem. 51, 1432-1442.
43. Okabe, A., Momota, Y., Yoshida, S., Hirata, A., Ito, J., Nishino, H., and Shiosaka, S. (1996). Kindling induces neuropsin mRNA in the mouse brain. Brain Res. 728, 116-120.
44. Rajapakse, S., Ogiwara, K., Takano, N., Moriyama, A., and Takahashi, T. (2005). Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins. FEBS Lett. 579, 6879-6884.
45. Russin, D.J., Floyd, B.F., Toomey, T.P., Brady, A.M., and Awad, W.M. Jr. (1974). The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (pronase). Stabilization of the trypsin component by calcium and guanidine. J. Biol. Chem. 249, 6144-6148.
46. Shimizu, C., Yoshida, S., Shibata, M., Kato, K., Momota, Y., Matsumoto, K., Shiosaka, T., Midorikawa, R., Kamachi, T., Kawabe, A., and Shiosaka, S. (1998). Characterization of recombinant and brain neuropsin, a plasticity-related serine protease. J. Biol. Chem. 273, 11189-11196.
47. Shimizu-Okabe, C., Yousef, G.M., Diamandis, E.P., Yoshida, S., Shiosaka, S., and Fahnestock, M. (2001). Expression of the kallikrein gene family in normal and Alzheimer's disease brain. Neuroreport 12, 2747-2751.
48. Stamey, T.A., Yang, N., Hay, A.R., McNeal, J.E., Freiha, F.S., and Redwine, E. (1987). Prostate-specific antigen as a serum marker for adenocarcinoma of the prostate. N. Engl. J. Med. 317, 909-916.
49. Takayama, T.K., McMullen, B.A., Nelson, P.S., Matsumura, M., and Fujikawa, K. (2001). Characterization of hK4 (prostase), a prostate-specific serine protease: activation of the precursor of prostate specific antigen (pro-PSA) and single-chain urokinase-type plasminogen activator and degradation of prostatic acid phosphatase. Biochemistry 40, 15341-15348.
50. Umezawa, H. (1976). Structures and activities of protease inhibitors of microbial origin. Methods Enzymol. 45, 678-695.
51. Underwood, L.J., Tanimoto, H., Wang, Y., Shigemasa, K., Parmley, T.H., and O'Brien, T.J. (1999). Cloning of tumor-associated differentially expressed gene-14, a novel serine protease overexpressed by ovarian carcinoma. Cancer Res. 59, 4435-4439.
52. Valaitis, A.P. (1995). Gypsy moth midgut proteinases: purification and characterization of luminal trypsin, elastase and the brush border membrane leucine aminopeptidase. Insect Biochem. Mol. Biol. 25, 139-149.
53. Villoutreix, B.O., Getzoff, E.D., and Griffin, J.H. (1994). A structural model for the prostate disease marker, human prostate-specific antigen. Protein Sci. 3, 2033-2044.
54. Walt, H., Hornung, R., Fink, D., Dobler-Girdziunaite, D., Stallmach, T., Spycher, M.A., Maly, F., Haller, U., and Burki, N. (2001). Hypercalcemic-type of small cell carcinoma of the ovary: characterization of a new tumor line. Anticancer Res. 21, 3253-3259.
55. Watt, K.W., Lee, R.J., M'Timkulu, T., Chan, W.P., and Loor, R. (1986). Human prostate-specific antigen: structural and functional similarity with serine proteases. Proc. Natl. Acad. Sci. USA 83, 3166-3170.
56. Yoshida, S., Taniguchi, M., Hirata, A., and Shiosaka, S. (1998). Sequence analysis and expression of human neuropsin cDNA and gene. Gene 213, 9-16.
57. Yousef, G.M. and Diamandis, E.P. (2001). The new human tissue kallikrein gene family: structure, function, and association to disease. Endocr. Rev. 22, 184-204.
58. Yousef, G.M. and Diamandis, E.P. (2002). Human tissue kallikreins: a new enzymatic cascade pathway? Biol. Chem. 383, 1045-1057
59. Yousef, G.M., Polymeris, M.E., Grass, L., Soosaipillai, A., Chan, P.C., Scorilas, A., Borgono, C., Harbeck, N., Schmalfeldt, B., Dorn, J., et al. (2003). Human kallikrein 5: a potential novel serum biomarker for breast and ovarian cancer. Cancer Res. 63, 3958-3965.
60. Yousef, G.M., Borgono, C.A., Popalis, C., Yacoub, G.M., Polymeris, M.E., Soosaipillai, A., and Diamandis, E.P. (2004a). In silica analysis of kallikrein gene expression in pancreatic and colon cancers. Anticancer Res. 24, 43-51.
61. Yousef, G.M., Yacoub, G.M., Polymeris, M.E., Popalis, C., Soosaipillai, A., and Diamandis, E.P. (2004b). Kallikrein gene downregulation in breast cancer. Br. J. Cancer 90, 167-172.