메뉴 건너뛰기




Volumn 360, Issue 3, 2006, Pages 548-557

A Cytotoxic Ribonuclease Variant with a Discontinuous Nuclear Localization Signal Constituted by Basic Residues Scattered Over Three Areas of the Molecule

Author keywords

human pancreatic ribonuclease; importin ; molecular recognition; NLS; protein protein interactions

Indexed keywords

PROTEIN; PROTEIN PE5; RIBONUCLEASE; UNCLASSIFIED DRUG;

EID: 33745674259     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2006.05.048     Document Type: Article
Times cited : (20)

References (42)
  • 1
    • 0029797247 scopus 로고    scopus 로고
    • The hnRNP C proteins contain a nuclear retention sequence that can override nuclear export signals
    • Nakielny S., and Dreyfuss G. The hnRNP C proteins contain a nuclear retention sequence that can override nuclear export signals. J. Cell Biol. 134 (1996) 1365-1373
    • (1996) J. Cell Biol. , vol.134 , pp. 1365-1373
    • Nakielny, S.1    Dreyfuss, G.2
  • 2
    • 0025949412 scopus 로고
    • Nuclear targeting sequences-a consensus?
    • Dingwall C., and Laskey R.A. Nuclear targeting sequences-a consensus?. Trends Biochem. Sci. 16 (1991) 478-481
    • (1991) Trends Biochem. Sci. , vol.16 , pp. 478-481
    • Dingwall, C.1    Laskey, R.A.2
  • 3
    • 0026078249 scopus 로고
    • Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence
    • Robbins J., Dilworth S.M., Laskey R.A., and Dingwall C. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64 (1991) 615-623
    • (1991) Cell , vol.64 , pp. 615-623
    • Robbins, J.1    Dilworth, S.M.2    Laskey, R.A.3    Dingwall, C.4
  • 4
    • 0030221192 scopus 로고    scopus 로고
    • Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids
    • Makkerh J.P., Dingwall C., and Laskey R.A. Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr. Biol. 6 (1996) 1025-1027
    • (1996) Curr. Biol. , vol.6 , pp. 1025-1027
    • Makkerh, J.P.1    Dingwall, C.2    Laskey, R.A.3
  • 6
    • 0040251482 scopus 로고    scopus 로고
    • Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells
    • Jakel S., and Gorlich D. Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J. 17 (1998) 4491-4502
    • (1998) EMBO J. , vol.17 , pp. 4491-4502
    • Jakel, S.1    Gorlich, D.2
  • 7
    • 2542509375 scopus 로고    scopus 로고
    • Functional modules in ribosomal protein L5 for ribonucleoprotein complex formation and nucleocytoplasmic transport
    • Claussen M., Rudt F., and Pieler T. Functional modules in ribosomal protein L5 for ribonucleoprotein complex formation and nucleocytoplasmic transport. J. Biol. Chem. 274 (1999) 33951-33958
    • (1999) J. Biol. Chem. , vol.274 , pp. 33951-33958
    • Claussen, M.1    Rudt, F.2    Pieler, T.3
  • 8
    • 0035050656 scopus 로고    scopus 로고
    • Characterisation of nuclear localisation signals of the four human core histones
    • Baake M., Doenecke D., and Albig W. Characterisation of nuclear localisation signals of the four human core histones. J. Cell Biochem. 81 (2001) 333-346
    • (2001) J. Cell Biochem. , vol.81 , pp. 333-346
    • Baake, M.1    Doenecke, D.2    Albig, W.3
  • 9
    • 0029278621 scopus 로고
    • Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope
    • Gorlich D., Kostka S., Kraft R., Dingwall C., Laskey R.A., Hartmann E., et al. Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr. Biol. 5 (1995) 383-392
    • (1995) Curr. Biol. , vol.5 , pp. 383-392
    • Gorlich, D.1    Kostka, S.2    Kraft, R.3    Dingwall, C.4    Laskey, R.A.5    Hartmann, E.6
  • 10
    • 0031707505 scopus 로고    scopus 로고
    • Nucleocytoplasmic transport: the soluble phase
    • Mattaj I.W., and Englmeier L. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67 (1998) 265-306
    • (1998) Annu. Rev. Biochem. , vol.67 , pp. 265-306
    • Mattaj, I.W.1    Englmeier, L.2
  • 11
    • 0032950628 scopus 로고    scopus 로고
    • Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha
    • Kobe B. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha. Nature Struct. Biol. 6 (1999) 388-397
    • (1999) Nature Struct. Biol. , vol.6 , pp. 388-397
    • Kobe, B.1
  • 12
    • 0035203632 scopus 로고    scopus 로고
    • Transport into and out of the nucleus
    • Macara I.G. Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65 (2001) 570-594
    • (2001) Microbiol. Mol. Biol. Rev. , vol.65 , pp. 570-594
    • Macara, I.G.1
  • 13
    • 0032563246 scopus 로고    scopus 로고
    • Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha
    • Conti E., Uy M., Leighton L., Blobel G., and Kuriyan J. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell 94 (1998) 193-204
    • (1998) Cell , vol.94 , pp. 193-204
    • Conti, E.1    Uy, M.2    Leighton, L.3    Blobel, G.4    Kuriyan, J.5
  • 14
    • 0034646565 scopus 로고    scopus 로고
    • Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha
    • Fontes M.R., Teh T., and Kobe B. Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha. J. Mol. Biol. 297 (2000) 1183-1194
    • (2000) J. Mol. Biol. , vol.297 , pp. 1183-1194
    • Fontes, M.R.1    Teh, T.2    Kobe, B.3
  • 15
    • 0034653375 scopus 로고    scopus 로고
    • Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha
    • Conti E., and Kuriyan J. Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha. Structure 8 (2000) 329-338
    • (2000) Structure , vol.8 , pp. 329-338
    • Conti, E.1    Kuriyan, J.2
  • 16
    • 0041845285 scopus 로고    scopus 로고
    • Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha
    • Fontes M.R., Teh T., Jans D., Brinkworth R.I., and Kobe B. Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha. J. Biol. Chem. 278 (2003) 27981-27987
    • (2003) J. Biol. Chem. , vol.278 , pp. 27981-27987
    • Fontes, M.R.1    Teh, T.2    Jans, D.3    Brinkworth, R.I.4    Kobe, B.5
  • 17
    • 0142149147 scopus 로고    scopus 로고
    • Dissection of the karyopherin alpha nuclear localization signal (NLS)-binding groove: functional requirements for NLS binding
    • Leung S.W., Harreman M.T., Hodel M.R., Hodel A.E., and Corbett A.H. Dissection of the karyopherin alpha nuclear localization signal (NLS)-binding groove: functional requirements for NLS binding. J. Biol. Chem. 278 (2003) 41947-41953
    • (2003) J. Biol. Chem. , vol.278 , pp. 41947-41953
    • Leung, S.W.1    Harreman, M.T.2    Hodel, M.R.3    Hodel, A.E.4    Corbett, A.H.5
  • 18
    • 1442300945 scopus 로고    scopus 로고
    • A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity
    • Bosch M., Benito A., Ribó M., Puig T., Beaumelle B., and Vilanova M. A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity. Biochemistry 43 (2004) 2167-2177
    • (2004) Biochemistry , vol.43 , pp. 2167-2177
    • Bosch, M.1    Benito, A.2    Ribó, M.3    Puig, T.4    Beaumelle, B.5    Vilanova, M.6
  • 19
    • 0033213099 scopus 로고    scopus 로고
    • Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability
    • Canals A., Ribó M., Benito A., Bosch M., Mombelli E., and Vilanova M. Production of engineered human pancreatic ribonucleases, solving expression and purification problems, and enhancing thermostability. Protein Expr. Purif. 17 (1999) 169-181
    • (1999) Protein Expr. Purif. , vol.17 , pp. 169-181
    • Canals, A.1    Ribó, M.2    Benito, A.3    Bosch, M.4    Mombelli, E.5    Vilanova, M.6
  • 20
    • 0034331073 scopus 로고    scopus 로고
    • Finding nuclear localization signals
    • Cokol M., Nair R., and Rost B. Finding nuclear localization signals. EMBO Rep. 1 (2000) 411-415
    • (2000) EMBO Rep. , vol.1 , pp. 411-415
    • Cokol, M.1    Nair, R.2    Rost, B.3
  • 21
    • 0032972509 scopus 로고    scopus 로고
    • PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization
    • Nakai K., and Horton P. PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem. Sci. 24 (1999) 34-36
    • (1999) Trends Biochem. Sci. , vol.24 , pp. 34-36
    • Nakai, K.1    Horton, P.2
  • 22
    • 0027937653 scopus 로고
    • Isolation of a protein that is essential for the first step of nuclear protein import
    • Gorlich D., Prehn S., Laskey R.A., and Hartmann E. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79 (1994) 767-778
    • (1994) Cell , vol.79 , pp. 767-778
    • Gorlich, D.1    Prehn, S.2    Laskey, R.A.3    Hartmann, E.4
  • 23
    • 0035823482 scopus 로고    scopus 로고
    • Biophysical characterization of interactions involving importin-alpha during nuclear import
    • Catimel B., Teh T., Fontes M.R., Jennings I.G., Jans D.A., Howlett G.J., et al. Biophysical characterization of interactions involving importin-alpha during nuclear import. J. Biol. Chem. 276 (2001) 34189-34198
    • (2001) J. Biol. Chem. , vol.276 , pp. 34189-34198
    • Catimel, B.1    Teh, T.2    Fontes, M.R.3    Jennings, I.G.4    Jans, D.A.5    Howlett, G.J.6
  • 24
    • 0037455715 scopus 로고    scopus 로고
    • An extended bipartite nuclear localization signal in Smad4 is required for its nuclear import and transcriptional activity
    • Xiao Z., Latek R., and Lodish H.F. An extended bipartite nuclear localization signal in Smad4 is required for its nuclear import and transcriptional activity. Oncogene 22 (2003) 1057-1069
    • (2003) Oncogene , vol.22 , pp. 1057-1069
    • Xiao, Z.1    Latek, R.2    Lodish, H.F.3
  • 25
    • 0031473847 scopus 로고    scopus 로고
    • SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling
    • Guex N., and Peitsch M.C. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18 (1997) 2714-2723
    • (1997) Electrophoresis , vol.18 , pp. 2714-2723
    • Guex, N.1    Peitsch, M.C.2
  • 26
    • 0042622380 scopus 로고    scopus 로고
    • SWISS-MODEL: an automated protein homology-modeling server
    • Schwede T., Kopp J., Guex N., and Peitsch M.C. SWISS-MODEL: an automated protein homology-modeling server. Nucl. Acids Res. 31 (2003) 3381-3385
    • (2003) Nucl. Acids Res. , vol.31 , pp. 3381-3385
    • Schwede, T.1    Kopp, J.2    Guex, N.3    Peitsch, M.C.4
  • 27
    • 0026723508 scopus 로고
    • The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors
    • Moore M.S., and Blobel G. The two steps of nuclear import, targeting to the nuclear envelope and translocation through the nuclear pore, require different cytosolic factors. Cell 69 (1992) 939-950
    • (1992) Cell , vol.69 , pp. 939-950
    • Moore, M.S.1    Blobel, G.2
  • 28
    • 0031779831 scopus 로고    scopus 로고
    • Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro
    • Kehlenbach R.H., Dickmanns A., and Gerace L. Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro. J. Cell Biol. 141 (1998) 863-874
    • (1998) J. Cell Biol. , vol.141 , pp. 863-874
    • Kehlenbach, R.H.1    Dickmanns, A.2    Gerace, L.3
  • 29
    • 0018987976 scopus 로고
    • Intracellular protein topogenesis
    • Blobel G. Intracellular protein topogenesis. Proc. Natl Acad. Sci. USA 77 (1980) 1496-1500
    • (1980) Proc. Natl Acad. Sci. USA , vol.77 , pp. 1496-1500
    • Blobel, G.1
  • 30
    • 0035696925 scopus 로고    scopus 로고
    • Core histones and linker histones are imported into the nucleus by different pathways
    • Baake M., Bauerle M., Doenecke D., and Albig W. Core histones and linker histones are imported into the nucleus by different pathways. Eur. J. Cell Biol. 80 (2001) 669-677
    • (2001) Eur. J. Cell Biol. , vol.80 , pp. 669-677
    • Baake, M.1    Bauerle, M.2    Doenecke, D.3    Albig, W.4
  • 31
    • 0034859839 scopus 로고    scopus 로고
    • Multiple pathways contribute to nuclear import of core histones
    • Muhlhausser P., Muller E.C., Otto A., and Kutay U. Multiple pathways contribute to nuclear import of core histones. EMBO Rep. 2 (2001) 690-696
    • (2001) EMBO Rep. , vol.2 , pp. 690-696
    • Muhlhausser, P.1    Muller, E.C.2    Otto, A.3    Kutay, U.4
  • 32
    • 27244461123 scopus 로고    scopus 로고
    • Identification of novel nuclear localization signal within the ErbB-2 protein
    • Chen Q.Q., Chen X.Y., Jiang Y.Y., and Liu J. Identification of novel nuclear localization signal within the ErbB-2 protein. Cell Res. 15 (2005) 504-510
    • (2005) Cell Res. , vol.15 , pp. 504-510
    • Chen, Q.Q.1    Chen, X.Y.2    Jiang, Y.Y.3    Liu, J.4
  • 33
    • 0035847008 scopus 로고    scopus 로고
    • Dissection of a nuclear localization signal
    • Hodel M.R., Corbett A.H., and Hodel A.E. Dissection of a nuclear localization signal. J. Biol. Chem. 276 (2001) 1317-1325
    • (2001) J. Biol. Chem. , vol.276 , pp. 1317-1325
    • Hodel, M.R.1    Corbett, A.H.2    Hodel, A.E.3
  • 34
    • 0028121484 scopus 로고
    • Improved efficiency in site-directed mutagenesis by PCR using a Pyrococcus sp. GB-D polymerase
    • Juncosa-Ginesta M., Pons J., Planas A., and Querol E. Improved efficiency in site-directed mutagenesis by PCR using a Pyrococcus sp. GB-D polymerase. Biotechniques 16 (1994) 820-823
    • (1994) Biotechniques , vol.16 , pp. 820-823
    • Juncosa-Ginesta, M.1    Pons, J.2    Planas, A.3    Querol, E.4
  • 36
    • 0024448151 scopus 로고
    • Calculation of protein extinction coefficients from amino acid sequence data
    • Gill S.C., and von Hippel P.H. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182 (1989) 319-326
    • (1989) Anal. Biochem. , vol.182 , pp. 319-326
    • Gill, S.C.1    von Hippel, P.H.2
  • 38
    • 0031916828 scopus 로고    scopus 로고
    • The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties
    • Efthymiadis A., Briggs L.J., and Jans D.A. The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties. J. Biol. Chem. 273 (1998) 1623-1628
    • (1998) J. Biol. Chem. , vol.273 , pp. 1623-1628
    • Efthymiadis, A.1    Briggs, L.J.2    Jans, D.A.3
  • 39
    • 0032483544 scopus 로고    scopus 로고
    • Crystal structure of a Smad MH1 domain bound to DNA: insights on DNA binding in TGF-beta signaling
    • Shi Y., Wang Y.F., Jayaraman L., Yang H., Massague J., and Pavletich N.P. Crystal structure of a Smad MH1 domain bound to DNA: insights on DNA binding in TGF-beta signaling. Cell 94 (1998) 585-594
    • (1998) Cell , vol.94 , pp. 585-594
    • Shi, Y.1    Wang, Y.F.2    Jayaraman, L.3    Yang, H.4    Massague, J.5    Pavletich, N.P.6
  • 40
    • 0034644763 scopus 로고    scopus 로고
    • Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases
    • Pous J., Canals A., Terzyan S.S., Guasch A., Benito A., Ribó M., et al. Three-dimensional structure of a human pancreatic ribonuclease variant, a step forward in the design of cytotoxic ribonucleases. J. Mol. Biol. 303 (2000) 49-60
    • (2000) J. Mol. Biol. , vol.303 , pp. 49-60
    • Pous, J.1    Canals, A.2    Terzyan, S.S.3    Guasch, A.4    Benito, A.5    Ribó, M.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.