Effects of Hydrodynamics on Aggregates Formation, Growth and Metabolism of HEK 293 Cells in Suspension Culture

Chinese Journal of Biotechnology

Volumn 22, Issue 1, 2006, Pages 101-106

  LIU, Hong ^a   LIU, Xing Mao ^a   WU, Ben Chuan ^a   YE, Ling Ling ^a   NI, Xiao Ping ^a   WANG, Qi Wei ^a   CHEN, Zhao Lie ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

Author keywords

aggregates; growth; HEK 293 cells; hydrodynamics; metabolism

Indexed keywords

ARTICLE; BIOREACTOR; CELL AGGREGATION; CELL LINE; CELL PROLIFERATION; CULTURE TECHNIQUE; HUMAN; INSTRUMENTATION; KINETICS;

BIOREACTORS; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; HUMANS; KINETICS;

EID: 33745493962     PISSN: 18722075     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1872-2075(06)60007-1     Document Type: Article

References (16)

1. Côte J., Garnier A., Massie B., and Kamen A. Serum-free production of recombinant proteins and adenoviral vector by 293SF-3F6 cells. Biotechnol Bioeng 59 (1998) 567-575
2. Wurn F., and Bernard A. Large-scale transient expression in mammalian cells for recombinant protein production. Curr Opin Biotechnol 10 (1999) 156-159
3. Nadeau I., and Kamen A. Production of adenovirus vector for gene therapy. Biotechnol Adv 20 (2003) 475-489
4. Peshwa M., Kyung Y., McClure D., and Hu W. Cultivation of mammalian cells as aggregates in bioreactors: effect of calcium concentration on spatial distribution of viability. Biotechnol Bioeng 41 (1993) 179-187
5. Zhao L., Zhu M., Zhang X., and Tan W. Effect of calcium on aggregation and growth of 293 cells. Chin J Biotech 21 (2005) 482-485
6. Moreira J., Alves P., Aunins J., and Carrondo M. Changes in animal cell natural aggregates in suspended batch cultures. Appl Microbiol Biotechnol 41 (1994) 203-209
7. Moreira J., Santana P., Feliciano A., Cruz P., Racher A., Griffiths J., and Carrondo M. Effect of viscosity upon hydro- dynamically controlled natural aggregates of animal cells grown in stirred vessels. Biotechnol Prog 11 (1995) 575-583
8. Chalmers J. Animal cell culture, effects of agitation and aeration on cell adaptation. In: Spier R. (Ed). Encyclopedia of cell technology vol 1 (2000), Wiley, New York 41-51
9. Puthli M., Rathod V., and Pandit A. Gas-liquid mass transfer studies with triple impeller system on a laboratory scale bioreactor. Biochemical Engineering Journal 23 1 (2005) 25-30
10. Moreira J., and Alves P. Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol Bioeng 46 (1995) 351-360
11. Sinskey A., Fleischaker R., Tyo M., Giard D., and Wang D. Production of cell-derived products: virus and interferon. Ann NY Acad Sci 369 (1981) 47-59
12. Goetgheheur S., and Hu W. Cultivation of anchorage-dependedt animal cells in microspherev induced aggregate culture. Appl Microbiol Biotechnol 34 (1991) 735-741
13. Moreira J., Alves P., Rodrigues J., Cruz P., Aunins J., and Carrondo M. Studies of baby hamster kidney (BHK) natural cell aggregates in suspended batch cultures. Ann NY Acad Sci 745 (1994) 122-133
14. Renner W., Jordan M., Eppenberger H., and Leist C. Cell-cell adhesion and aggregation: influence on the growth behavior of CHO cells. Biotechnol Bioeng 41 (1993) 188-193
15. Sutherland R. Cell and environment interactions in tumor microregions: The Multicell Spheroid Model. Science 240 (1988) 177-184
16. Knuechel R., Keng P., Hofstaedter F., Lagmuri V., Sutherland R., and Penney D. Differentiation patterns in two- and three- dimensional culture systems of human squamous carcinoma cell lines. Am J Pathol 137 (1990) 725-736