Carotenoid stoichiometry in the LH2 crystal: No spectral evidence for the presence of the second molecule in the α/β-apoprotein dimer

FEBS Letters

Volumn 580, Issue 16, 2006, Pages 3841-3844

  Gall, Andrew ^a   Gardiner, Alastair T ^b   Cogdell, Richard J ^b   Robert, Bruno ^a  

^a CNRS   (France)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Bacteriochlorophyll; Carotenoid; Crystallography; Photosynthesis; Pigment protein; Raman spectroscopy

Indexed keywords

APOLIPOPROTEIN A; APOLIPOPROTEIN B; CAROTENOID; HETERODIMER;

ARTICLE; LIGHT HARVESTING SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; RAMAN SPECTROMETRY; RHODOBLASTUS ACIDOPHILUS;

APOPROTEINS; BACTERIAL PROTEINS; CAROTENOIDS; CRYSTALLIZATION; DIMERIZATION; LIGHT-HARVESTING PROTEIN COMPLEXES; RHODOPSEUDOMONAS; SPECTRUM ANALYSIS, RAMAN; TEMPERATURE;

RHODOBLASTUS ACIDOPHILUS;

EID: 33745424153     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.06.005     Document Type: Article

References (28)

1. Zuber H., and Cogdell R.J. Structure and organization of purple bacterial antenna complexes. In: Blakenship R.E., Madigan M.T., and Bauer C.E. (Eds). Anoxygenic Photosynthetic Bacteria Vol. 2 (1995), Kluwer Academic Publishers 315-348
2. Imhoff J.F. Transfer of Rhodopseudomonas acidophila to the new genus Rhodoblastus as Rhodoblastus acidophilus gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 51 (2001) 1863-1866
3. McDermott G., Prince S.M., Freer A.A., Hawthornthwaite-Lawless A.M., Papiz M.Z., Cogdell R.J., and Isaacs N.W. Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374 (1995) 517-521
4. Ohashi N., KoChi N., Kuki M., Shimamura T., Cogdell R.J., and Koyama Y. The structures of S-0 spheroidene in the light-harvesting (LH2) complex and S-0 and T-1 spheroidene in the reaction center of Rhodobacter sphaeroides 2.4.1 as revealed by Raman spectroscopy. Biospectroscopy 2 (1996) 59-69
5. Robert B. Resonance Raman studies in photosynthesis-chlorophyll and carotenoid molecules. In: Amez A., and Hoff A. (Eds). Biophysical Techniques in Photosynthesis (1996), Kluwer Academic Publishers, Amsterdam 161-276
6. Robert B., and Frank H.A. A resonance Raman investigation of the effect of lithium dodecyl sulfate on the B800-850 light-harvesting protein of Rhodopseudomonas acidophila 7750. Biochim. Biophys. Acta 934 (1988) 401-405
7. Papiz M.Z., Prince S.M., Howard T., Cogdell R.J., and Isaacs N.W. The structure and thermal motion of the B800-850 LH2 complex from Rps. acidophila at 2.0 Å resolution and 100 K: new structural features and functionally relevant motions. J. Mol. Biol. 326 (2003) 1523-1538
8. Koyama Y., Takii T., Saiki K., and Tsukida K. Configuration of the carotenoid in the reaction centers of photosynthetic bacteria. 2. Comparison of the resonance Raman lines of the reaction centers with those of the 14 different cis-trans isomers of β-carotene. Photobiochem. Photobiophys. 5 (1983) 139-150
9. Robert B. The electronic structure, stereochemistry and resonance Raman spectroscopy of carotenoids. In: Frank H.A., Young A.J., Britton G., and Cogdell R.J. (Eds). The Photochemistry of Carotenoids Vol. 8 (1999), Kluwer Academic Publisher, Dordrecht 189-201
10. Pfenning N. Rhodopseudomonas acidophila, sp. n., a new species of budding purple nonsulfur bacteria. J. Bacteriol. 99 (1969) 597-602
11. Papiz M.Z., Hawthornthwaite A.M., Cogdell R.J., Woolley K.J., Wightman P.A., Ferguson L.A., and Lindsay J.G. Crystallization and characterization of two crystal forms of the B800-850 light-harvesting complex from Rhodopseudomonas acidophila strain 10050. J. Mol. Biol. 209 (1989) 833-835
12. Howard T.D., McAuley-Hecht K.E., and Cogdell R.J. Crystallization of membrane proteins. Membrane Transport (2000), Oxford University Press, Oxford, UK 269-307
13. Koyama Y., Kito M., Takii T., Saiki K., Tsukida K., and Yamashita J. Configuration of the carotenoid in the reaction centers of photosynthetic bacteria. Comparison of the resonance Raman spectrum of the reaction centers of Rhodopseudomonas sphaeroides G1C with those of cis-trans isomers of β-carotene. Biochim. Biophys. Acta 680 (1982) 109-118
14. Robert B., Cogdell R.J., and Van Grondelle R. The light-harvesting system of purple bacteria. In: Parson W.W., and Green B.B. (Eds). Light-harvesting Antennas in Photosynthesis Vol. 13 (2003), Kluwer Academic Publishers, Dordrecht 169-194
15. Rimai L., Gill D., and Parson J.L. Raman spectra of dilute solutions of some stereoisomers of vitamin A-type molecules. J. Am. Chem. Soc. 93 (1971) 1353-1357
16. Saito S., and Tasumi M. Normal-coordinate analysis of β-carotene isomers and assignments of the Raman and infrared bands. J. Raman Spec. 14 (1983) 310-321
17. Koyama Y., Long R.A., Martin W.G., and Carey P.R. The resonance Raman spectrum of carotenoids as an intrinsic probe for membrane potential. Oscillatory changes in the spectrum of neurosporene in the chromatophores of Rhodopseudomonas sphaeroides. Biochim. Biophys. Acta 548 (1979) 153-160
18. Lutz M., Szponarski W., Berger G., Robert B., and Neumann J.M. The stereoisomerism of bacterial, reaction-center-bound carotenoids revisited: an electronic absorption, resonance Raman and proton NMR study. Biochim. Biophys. Acta 894 (1987) 423-433
19. Fyfe P.K. Biophysical Investigation of Photosynthetic Reaction Centres from Rhodobacter sphaeroides (1997), University of Glasgow, Glasgow
20. Fyfe P.K., McAuley-Hecht K.E., Ridge J.P., Prince S.M., Fritzsch G., Isaacs N.W., Cogdell R.J., and Jones M.R. Crystallographic studies of mutant reaction centers from Rhodobacter sphaeroides. Photosynth. Res. 55 (1998) 133-140
21. Gall A., Ridge J.P., Robert B., Cogdell R.J., Jones M.R., and Fyfe P.K. Effects of mutagenesis on the detailed structure of spheroidenone in the Rhodobacter sphaeroides reaction centre examined by resonance Raman spectroscopy. Photosynth. Res. 59 (1999) 223-230
22. Ruban A.V., Lee P.J., Wentworth M., Young A.J., and Horton P. Determination of the stoichiometry and strength of binding of xanthophylls to the photosystem II light harvesting complexes. J. Biol. Chem. 274 (1999) 10458-10465
23. Ruban A.V., Pascal A.A., Robert B., and Horton P. Configuration and dynamics of xanthophylls in light-harvesting antennae of higher plants. Spectroscopic analysis of isolated light-harvesting complex of photosystem II and thylakoid membranes. J. Biol. Chem. 276 (2001) 24862-24870
24. Ruban A.V., Pascal A., Lee P.J., Robert B., and Horton P. Molecular configuration of xanthophyll cycle carotenoids in photosystem II antenna complexes. J. Biol. Chem. 277 (2002) 42937-42942
25. Prince S.M., Howard T.D., Myles D.A.A., Wilkinson C., Papiz M.Z., Freer A.A., Cogdell R.J., and Isaacs N.W. Detergent structure in crystals of the integral membrane light-harvesting complex LH2 from Rhodopseudomonas acidophila strain 10050. J. Mol. Biol. 326 (2003) 307-315
26. Gall A., Henry S., Takaichi S., Robert B., and Cogdell R.J. Preferential incorporation of coloured-carotenoids occurs in the LH2 complexes from non-sulphur purple bacteria under carotenoid-limiting conditions. Photosynth. Res. 86 (2005) 25-35
27. Arellano J.B., Raju B.B., Naqvi K.R., and Gillbro T. Estimation of pigment stoichiometries in photosynthetic systems of purple bacteria: special reference to the (absence of) second carotenoid in LH2. Photochem. Photobiol. 68 (1998) 84-87
28. Cherezov V., Clogston J., Papiz M.Z., and Caffrey M. Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J. Mol. Biol. 357 (2006) 1605-1618