Photo-bleaching compensation for autofocus algorithms in fluorescence microscope applications

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 6142 III, Issue , 2006, Pages

  Soda, Paolo ^a   Iannello, Giulio ^a  

^a UNIVERSITY CAMPUS BIO MEDICO   (Italy)

Author keywords

Autofocus algorithms; Automatic image acquisition; Fluorescence microscopy; Focus criteria

Indexed keywords

ALGORITHMS; FLUORESCENCE; IMAGE PROCESSING; IMAGE QUALITY; MICROSCOPIC EXAMINATION;

AUTOFOCUS ALGORITHMS; AUTOMATIC IMAGE ACQUISITION; FLUORESCENCE MICROSCOPY; FOCUS CRITERIA; HISTOGRAM; IMMUNOFLUORESCENCE ASSAY (IFA); PHOTO-BLEACHING;

MEDICAL IMAGING;

EID: 33745417172     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.650096     Document Type: Conference Paper

References (12)

1. A. Kavanaugh, R. Tomar, J. Reveille, et al., "Guidelines for Clinical Use of the Antinuclear Antibody Test and Tests for Specific Autoantibodies to Nuclear Antigens", American College of Pathologists, Arch Pathol Lab Med, Vol. 124, pp. 71-81, 2000.
2. R. Marcolongo et al., "Presentazione Linee Guida del Forum Interdisciplinare per la Ricerca sulle Malattie Autoimmuni (F.I.R.M.A.)", Reumatismo, Vol. 55, pp. 9-21, 2003.
3. "Service Manual AP16 IF Plus", Das s.r.l., Palombara Sabina (RI), March 2004.
4. "PhD System", Bio-Rad Laboratories Inc., Available: http://www.bio-rad.com, 2004.
5. F.C.A. Groen, I.T. Young, G.A. Lighthard, "A Comparison of Different Focus Functions for Use in Autofocus Algorithms", Cytometry, Vol. 6, pp. 81-91,1985.
6. L. Firestone, K. Cook, K. Culp, N. Talsania, K. Preston jr, "Comparison of Autofocus Methods for Automated Microscopy", Cytometry, Vol. 12, pp. 195-206, 1991.
7. A. Santos, C. Ortiz De Solorzano, J.J. Vaquero, J.M. Pena, N. Malpica, F. Del Pozo, "Evaluation of Autofocus Functions in Molecular Cytogenetic Analysis", The Royal Microscopical Society, Journal of Microscopy, Vol. 188, pp. 264-27, 1997.
8. J-M Geusebroek, F. Cornelissen, W.M. Arnold Smeulders, H. Geerts, "Robust Autofocusing in Microscopy", Cytometry, Vol. 39, pp. 1-9, 1999.
9. F.R. Boddeke, L.J. Van Vliet, H. Netten, I.T. Young, "Autofocusing in Microscopy Based on the OTF and Sampling", Biolmaging, Vol. 2, pp. 193-203, 1994.
10. W. Xiaoqiang, L. Xinmin, Z. Yiming, T. Deyuan, H. Xiaohai, T. Qizhi, "Autofocus Methods for Automated Microscopy", Proc. SPIE Vol. 4224, pp. 114-117, Biomedical Photonics and Optoelectronic Imaging, 2000.
11. L. Song, E.J. Hennink, I.T. Young, H.J. Tanke, "Photobleaching Kinetics of Fluorescein in Quantitative Fluorescence Microscopy", Biophysical Journal, Vol. 68, pp. 2588-2600, 1995.
12. D.H. Solomon, A. Kavanaugh, et al., "Evidence-Based Guidelines for the Use of Immunologie Tests: Antinuclear Antibody Testing", Arthritis & Rheumatism, Vol. 47, No.4, pp. 434-444, 2002.