Novel two-stage screening procedure leads to the identification of a new class of transfection enhancers

Journal of Gene Medicine

Volumn 8, Issue 6, 2006, Pages 745-753

  Neukamm, Birgit ^a   Weimann, Andreas ^b   Wu, Shuling ^b   Danevad, Margrete ^b   Lang, Christine ^a   Geßner, Reinhard ^b  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Chloroquine; DEAE dextran; Endocytosis; Gene transfer; Transfection enhancers; Tricyclic antidepressants

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AMANTADINE; AMITRIPTYLINE; AMMONIUM CHLORIDE; AMOXAPINE; BAFILOMYCIN A1; CHLOROQUINE; CHLORPHENIRAMINE; CHLORPROMAZINE; CLOMIPRAMINE; DESIPRAMINE; DNA; DOXEPIN; IMIDAZOLE; IMIPRAMINE; LUCIFERASE; MAPROTILINE; MEPACRINE; NORTRIPTYLINE; PHENYLGLYOXAL; PRIMAQUINE; PROPRANOLOL; PROPYLAMINE; PROTRIPTYLINE; PUTRESCINE; TILORONE; TRICYCLIC ANTIDEPRESSANT AGENT; TRIMIPRAMINE; TRIMIPRAMINE MALEATE; UNINDEXED DRUG; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; AUTOMATION; CELL ASSAY; CELL GROWTH; CELL LINE; CELL STRAIN HEPG2; CELL SURVIVAL; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DRUG SCREENING; ENDOCYTOSIS; GENE TRANSFER; GENETIC TRANSFECTION; GROWTH INHIBITION; HUMAN; HUMAN CELL; IC 50; MAMMAL CELL; MOLECULAR LIBRARY; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; REPORTER GENE; SACCHAROMYCES CEREVISIAE; TARGET CELL; VIRAL GENE DELIVERY SYSTEM; YEAST CELL;

ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; HUMANS; MAMMALS; SACCHAROMYCES CEREVISIAE; TRANSFECTION;

MAMMALIA;

EID: 33745286256     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.887     Document Type: Article

References (34)

1. Luo D, Saltzman WM. Enhancement of transfection by physical concentration of DNA at the cell surface. Nat Biotechnol 2000; 18: 893-895.
2. Assessment of adenoviral vector safety and toxicity: report of the National Institutes of Health Recombinant DNA Advisory Committee, NIH Report. Hum Gene Ther 2002; 13: 3-13.
3. FDA places temporary halt on gene therapy trials using retroviral vectors in blood stem cells. FDA talk paper 2003; T03-04.
4. Hacein-Bey-Abina S, Von Kalle C, Schmidt M, Le Deist F. A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. N Engl J Med 2003; 348: 255.
5. Neukamm B, Stahl U, Lang C. Endocytosis is involved in DNA uptake in yeast. Biochim Biophys Acta 2002; 1572: 67-76.
6. Luthman H, Magnusson G. High efficiency polyoma DNA transfection of chloroquine treated cells. Nucleic Acids Res 1983; 11: 1295-1308.
7. Wagner E, Curiel D, Cotton M. Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-induced endocytosis. Advanced Drug Delivery Rev 1994; 14: 113-135.
8. Vaheri A, Pagano JS. Infectious poliovirus RNA: a sensitive method of assay. Virology 1965; 27: 434-436.
9. Botstein D, Falco SC, Stewart SE, et al. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 1979; 8: 17-24.
10. Piper RC, Whitters EA, Stevens TH. Yeast Vps45p is a Sec1p like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles. Eur J Cell Biol 1994; 65: 305-318.
11. Lang C, Looman AC. Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 1995; 44: 147-156.
12. Melkonyan H, Sorg C, Klempt M. Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO). Nucleic Acids Res 1996; 24: 4356-4357.
13. Brachmann CB, Davies A, Cost GJ, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 1998; 14: 115-132.
14. Raths S, Rohrer J, Crausaz F, Riezman H. end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol 1993; 120: 55-65.
15. Guy J, Drabek D, Antonion M. Delivery of DNA into mammalian cells by receptor-mediated endocytosis and gene therapy. Mol Biotechnol 1995; 3: 237-248.
16. Erbacher P, Roche AC, Monsgny M, Midoux P. Putative role of chloroquine in gene transfer into a human hepatoma cell line by DNA/lactosylated polylysine complexes. Exp Cell Res 1996; 225: 186-194.
17. Mellman I, Fuchs R, Helenius A. Acidification of the endocytic and exocytic pathways. Annu Rev Biochem 1986; 55: 663-700.
18. Lechardeur D, Sohn KJ, Haardt M, et al. Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer. Gene Ther 1999; 64: 82-97.
19. Wattiaux R, Laurent N, Wattiaux-De Coninck S, Jadot M. Endosomes, lysosomes: their implication in gene transfer. Adv Drug Deliv Rev 2000; 41: 201-208.
20. Ciftci K, Levy RJ. Enhanced plasmid DNA transfection with lysosomotropic agents in cultured fibroblasts. Int J Pharm 2001; 218: 81-92.
21. Schneider P, Korolenko TA, Busch U. A review of drug-induced lysosomal disorders of the liver in man and laboratory animals. Microsc Res Technol 1997; 36: 253-275.
22. Potter WZ, Hollister LE. Antidepressant agents. In Basic and Clinical Pharmacology, Katzung BG (ed). Lange Medical Books/McGraw-Hill: San Francisco, 2001; 498-511.
23. Daniel WA, Wojcikowski J. Contribution of lysosomal trapping to the total tissue uptake of psychotropic drugs. Pharmacol Toxicol 1997; 80: 62-68.
24. Morvan F, Castex C, Vives E, Imbach JL. Polyimidazole conjugated oligonucleotides reach the nucleus of HeLa cells. Nucleosides Nucleotides Nucleic Acids 2001; 20: 805-808.
25. Baron PL, Meola G, Scarpini E, Moggio M, Santilli I, Scarlato G. Lysosomotropic agents induce morphological and functional changes in human muscle cells in vitro. Eur J Basic Appl Histochem 1999; 135: 423-432.
26. Doh-Ura K, Iwaki T, Caughey B. Lysosomotropic agents and cysteine protease inhibitors inhibit scrapie-associated prion protein accumulation. J Virol 2000; 74: 4894-4897.
27. Helenius A, Marsh M, White J. Inhibition of Semliki forest virus penetration by lysosomotropic weak bases. J Gen Virol 1982; 58: 47-61.
28. Seglen PO, Gordon PB, Poli A. Amino acid inhibition of the autophagic/ lysosomal pathway of protein degradation in isolated rat hepatocytes. Biochim Biophys Acta 1980; 630: 103-118.
29. Hawtrey A, Joubert D, Van Jaarsveld P, Pieterse A, van Zyl J, Ariatti M. Low concentrations of chlorpromazine and related phenothiazines stimulate gene transfer in HeLa cells via receptor-mediated endocytosis. Drug Deliv 2002; 9: 47-53.
30. Funhoff AM, van Nostrum F, Koning GA, Schuurmans-Nieuwenbroek NME, Crommelin DJA, Hennink WE. Endosomal escape of polymeric gene delivery complexes is not always enhanced by polymers buffering at low pH. Biomacromolecules 2004; 5: 32-39.
31. +-translocating adenosine triphosphatase in vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem 1981; 256: 10859-10863.
32. Bowman BJ, Bowman EJ. Mutations in subunit C of the vacuolar ATPase confer resistance to bafilomycin and identify a conserved antibiotic binding site. J Biol Chem 2002; 277: 3965-3972.
33. Waring M. In Progress in Molecular and Subcellular Biology, Hahn F (ed). Springer-Verlag: New York, 1971; 216-231.
34. Jones RL, Lanier AC, Keel RA, Wilson WD. The effect of ionic strength on DNA-ligand unwinding angles for acridine and quinoline derivatives. Nucleic Acids Res 1980; 11: 1613-1624.