Isolation in cell culture and detection by PCR-based technology of IPNV-like virus from leucocytes of carrier turbot, Scophthalmus maximus (L.)

Journal of Fish Diseases

Volumn 28, Issue 12, 2005, Pages 713-722

  Cutrin J M ^a   Lopez Vazquez C ^a   Olveira, J G ^a   Castro, S ^a   Dopazo, C P ^a   Bandin I ^a,b  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b Santiago de Compostela Spain   (Spain)

Author keywords

Carrier turbot; Infectious pancreatic necrosis virus; Nested polymerase chain reaction; Non destructive diagnosis; Reverse transcriptase polymerase chain reaction; Southern blot hybridization

Indexed keywords

PRIMER DNA;

BLOOD; DNA FINGERPRINTING; FLATFISH; HOST-PATHOGEN INTERACTION; NONDESTRUCTIVE TESTING; POLYMERASE CHAIN REACTION; POLYMORPHISM; VIRAL DISEASE;

ANIMAL; ANIMAL DISEASE; AQUACULTURE; ARTICLE; CLUSTER ANALYSIS; COMPARATIVE STUDY; DNA SEQUENCE; FISH DISEASE; FLATFISH; GENETICS; INFECTIOUS PANCREATIC NECROSIS VIRUS; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; SPAIN; VIREMIA; VIROLOGY; VIRUS INFECTION;

ANIMALS; AQUACULTURE; BASE SEQUENCE; BIRNAVIRIDAE INFECTIONS; BLOTTING, SOUTHERN; CLUSTER ANALYSIS; DNA PRIMERS; FISH DISEASES; FLATFISHES; INFECTIOUS PANCREATIC NECROSIS VIRUS; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPAIN; VIREMIA;

INFECTIOUS PANCREATIC NECROSIS VIRUS; SCOPHTHALMUS MAXIMUS;

EID: 33745281692     PISSN: 01407775     EISSN: 13652761     Source Type: Journal    
DOI: 10.1111/j.1365-2761.2005.00675.x     Document Type: Article

References (38)

1. Agius C. Mangunwiryo H. Johnson R.H. Smail D.A. ( 1982) A more sensitive technique for isolating infectious pancreatic necrosis virus from asymptomatic carrier rainbow trout, Salmo gairdneri Richardson. Journal of Fish Diseases 5, 285 292.
2. Ahne W. Thomsen I. ( 1986) Infectious pancreatic necrosis detection of virus and antibodies in rainbow trout IPNV carriers (Salmo gairdneri). Journal of Veterinary Medicine B 33, 552 554.
3. Blake S.L. Schill W.B. McAllister P.E. Lee M.-K. Singer J.T. Nicholson B.L. ( 1995) Detection and identification of aquatic birnaviruses by PCR assay. Journal of Clinical Microbiology 33, 835 839.
4. Blake S. Ma J.-Y. Caporale D.A. Jairath S. Nicholson B.L. ( 2001) Phylogenetic relationships of aquatic birnaviruses based on deduced amino acid sequences of genome segment A cDNA. Diseases of Aquatic Organisms 45, 89 102.
5. Castric J. Baudin-Laurencin F. Coustans M.F. Auffret M. ( 1987) Isolation of infectious pancreatic necrosis virus Ab serotype, from an epizootic in farmed turbot, Scophthalmus maximus. Aquaculture 67, 117 126.
6. Cutrín J.M. Olveira J.G. Barja J.L. Dopazo C.P. ( 2000) Diversity of infectious pancreatic necrosis virus strains isolated from fish, shellfish, and other reservoirs in northwestern Spain. Applied and Environmental Microbiology 66, 839 843.
7. Cutrín J.M. Barja J.L. Nicholson B.L. Bandín I. Blake S. Dopazo C.P. ( 2004) Restriction fragment length polymorphisms and sequence analysis: an approach for genotyping infectious pancreatic necrosis virus reference strains and other aquabirnavirus isolated from northwestern Spain. Applied and Environmental Microbiology 70, 1059 1067.
8. Dopazo C.P. Barja J.L. ( 2002) Diagnosis and identification of IPNV in salmonids by molecular methods. In: Molecular Diagnosis of Salmonid Diseases ( ed. by C.O. Cunningham pp. 23 48. Kluwer Academic Publishers, The Netherlands.
9. Duncan R. Dobos P. ( 1986) The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals a large ORF encoding a precursor polyprotein. Nucleic Acids Research 14, 5934 5935.
10. Duncan R. Mason C.L. Nagy E. Leong J.-A. Dobos P. ( 1991) Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif. Virology 181, 541 552.
11. Gahlawat S.K. Munro E.S. Ellis A.E. ( 2004) A non-destructive test for detection of IPNV-carriers in Atlantic halibut, Hippoglossus hippoglossus (L.). Journal of Fish Diseases 27, 233 239.
12. Gray W.L. Mullis L. LaPatra S.E. Groff J.M. Goodwin A. ( 2002). Detection of koi herpesvirus DNA in tissues of infected fish. Journal of Fish Diseases 25, 171 178.
13. Hill B.J. Way K. ( 1995) Serological classification of infectious pancreatic necrosis (IPN) virus and other aquatic birnaviruses. In : Annual Review of Fish Diseases (ed. by M. Faisal F.M. Hetrick Vol. 5, pp. 55 77 Pergamon (Elsevier), UK.
14. Johansen L.-H. Sommer A.I. ( 1995) Multiplication of infectious pancreatic necrosis virus (IPNV) in head kidney and blood leucocytes isolated from Atlantic salmon, Salmo salar L. Journal of Fish Diseases 18, 147 156.
15. Kim C.K Kim P.K. Sohn S.G. Sim D.S. Park M.A. Heo M.S. Lee T.H. Lee J.D. Jun H.K. Jang K.L. ( 1998) Development of a polymerase chain reaction (PCR) procedure for the detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimp. Journal of Fish Diseases 21, 11 17.
16. Ledo A. Lupiani B. Dopazo C.P. Toranzo A.E. Barja J.L. ( 1990) Fish viral infections in the Northwest of Spain. Microbiología 6, 21 29.
17. López-Lastra M. Gonzalez M. Jashes M. Sandino A.M. ( 1994) A detection method for infectious pancreatic necrosis virus (IPNV) based on reverse transcription (RT)-polymerase chain reaction (PCR). Journal of Fish Diseases 17, 269 282.
18. McAllister P.E. Schill W.B. Owens W.J. Hodge D.L. ( 1993) Determining the prevalence of infectious pancreatic necrosis virus in asymptomatic brook trout Salvelinus fontinalis: a study of clinical samples and processing methods. Diseases of Aquatic Organisms 15, 157 162.
19. Mortensen S.H. Hjeltnes B. Rodseth O. Krogsrud J. Christie K.E. ( 1990) Infectious pancreatic necrosis virus, serotype N1, isolated from Norwegian halibut (Hippoglossus hippoglossus), turbot (Scophthalmus maximus) and scallops (Pecten maximus). Bulletin of the European Association of Fish Pathologists 10, 42 43.
20. Munro E.S. Gahlawat S.K. Ellis A.E. ( 2004) A sensitive non-destructive method for detecting IPNV carrier Atlantic salmon, Salmo salar L., by culture of virus from plastic adherent blood leucocytes. Journal of Fish Diseases 27, 129 134.
21. Novoa B. Figueras A.J. Ledo A. Barja J.L. Toranzo A.E. ( 1991) Incidence of birnavirus in cultured turbot (Scophthalmus maximus L.) in Northwest Spain. FHS/AFS Newsletter 19, 2 3.
22. Novoa B. Toranzo A.E. Dopazo C.P. Barja J.L. Figueras A. ( 1993) Isolation of IPN virus serotype VR-299 from turbot in Europe. Diseases of Aquatic Organisms 17, 61 65.
23. Novoa B. Figueras A.J. Puentes C.F. Ledo A. Toranzo A.E. ( 1993) Characterization of a birnavirus isolated from diseased turbot cultured in Spain. Diseases of Aquatic Organisms 15, 163 169.
24. Novoa B. Blake S. Nicholson B.L. Figueras A. ( 1995) Application of 3 techniques for diagnosing birnavirus infection in turbot. Veterinary Research 26, 493 498.
25. OIE ( 2003) Manual of Diagnostic Test for Aquatic Animals, 4th edn. Office International des Epizooties, Paris.
26. Reno P.W. ( 1999) Infectious pancreatic necrosis and associated aquatic birnaviruses. In: Fish Diseases and Disorders Vol. 3: Viral, Bacterial and Fungal Infections ( ed. by P.T. Woo D.W. Bruno pp. 1 55. CABI Publishing, Wallingford, Oxon.
27. Rimstad E. Hornes E. Olsvik O.E. Hyllseth B. ( 1990) Identification of a double-stranded RNA virus by using polymerase chain reaction and magnetic separation of the synthesized DNA segments. Journal of Clinical Microbiology 28, 2275 2278.
28. Rodriguez S. Vilas P. Palacios M.A. Pérez-Prieto S. ( 1991) Detection of infectious pancreatic necrosis in a carrier population of rainbow trout, Oncorhynchus mykiss (Walbaum), by flow cytometry. Journal of Fish Diseases 14, 545 553.
29. Rodriguez S. Alonso M. Pérez-Prieto S. ( 2001) Detection of infectious pancreatic necrosis virus (IPNV) from leucocytes of carrier rainbow trout Oncorhynchus mykiss. Fish Pathology 36, 139 146.
30. Sanz F. Coll J. ( 1992) Techniques for diagnosing viral diseases of salmonid fish. Diseases of Aquatic Organisms 13, 211 223.
31. Smail D. Munro E.S. ( 2001) The virology of teleosts. In: Fish Pathology ( ed. by R.J. Roberts pp. 169 253. WB Saunders, London.
32. Smail D. Burnside K. Watt A. Munro E.S. ( 2003) Enhanced cell culture isolation of infectious pancreatic necrosis virus from kidney tissue of carrier Atlantic salmon (Salmo salar L.) using sonication of the cell harvest. Bulletin of the European Association of Fish Pathologists 23, 250 254.
33. Swanson R. Gillespie J. ( 1982) Isolation of infectious pancreatic necrosis virus from the blood components of experimentally infected trout. Canadian Journal of Fisheries and Aquatic Sciences 39, 225 228.
34. Taksdal T. Ramstad A. Stangeland K. Dannevig B.H. ( 1998) Induction of infectious pancreatic necrosis (IPN) in covertly infected Atlantic salmon, Salmo salar L., post-smolts by stress exposure, by injection of IPN virus (IPNV) and by cohabitation. Journal of Fish Diseases 21, 193 204.
35. Taksdal T. Dannevig B.H. Rimstad E. ( 2001) Detection of infectious pancreatic necrosis (IPN)-virus in experimentally infected Atlantic salmon parr by RT-PCR and cell culture isolation. Bulletin of the European Association of Fish Pathologists 21, 214 219.
36. Wang W.-S. Wi Y.-L. Lee J.-S. ( 1997) Single-tube, non-interrupted reverse transcription PCR for detection of infectious pancreatic necrosis virus. Diseases of Aquatic Organisms 28, 229 233.
37. Wolf K. ( 1988) Fish Viruses and Fish Viral Diseases. Cornell University Press, New York.
38. Yu K. McDonald R. Moore A. ( 1982) Replication of infectious pancreatic necrosis virus in trout leucocytes and detection of the carrier state. Journal of Fish Diseases 5, 401 410.