Characterization of Checkpoint Responses to DNA Damage in Saccharomyces cerevisiae: Basic Protocols

Methods in Enzymology

Volumn 409, Issue , 2006, Pages 101-117

  Pabla, Ritu ^a   Pawar, Vaibhav ^a   Zhang, Hong ^a   Siede, Wolfram ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CHECKPOINT KINASE 2; MUTANT PROTEIN;

CELL CYCLE; DNA DAMAGE; EUKARYOTE; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION; REVIEW; SACCHAROMYCES CEREVISIAE; YEAST;

BLOTTING, WESTERN; DNA DAMAGE; DNA, FUNGAL; FLOW CYTOMETRY; SACCHAROMYCES CEREVISIAE; ULTRAVIOLET RAYS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 33745216016     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(05)09006-3     Document Type: Review

References (23)

1. Allen J.B., Zhou Z., Siede W., Friedberg E.C., and Elledge S.J. The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage induced transcription in yeast. Genes Dev. 8 (1994) 2401-2415
2. Amberg D.C., Burke D.J., and Strathern J.N. "Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, 2005 Edition." (2005), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
3. Bennett C.B., Lewis L.K., Karthikeyan G., Lobachev K.S., Jin Y.H., Sterling J.F., Snipe J.R., and Resnick M.A. Genes required for ionizing radiation resistance in yeast. Nat. Genet. 29 (2001) 426-434
4. Clarke D.J., Segal M., Jensen S., and Reed S.I. Mec1p regulates Pds1p levels in S phase: Complex coordination of DNA replication and mitosis. Nature Cell Biol. 3 (2001) 619-627
5. Foiani M., Marini F., Gamba D., Lucchini G., and Plevani P. The B subunit of the DNA polymerase ∝-primase complex in Saccharomyces cerevisiae executes an essential function at the initial stage of DNA replication. Mol. Cell. Biol. 14 (1994) 923-933
6. Friedberg E.C., Walker G.C., Siede W., Wood R.D., Schultz R.A., and Ellenberger T. "DNA Repair and Mutagenesis." (2005), American Society of Microbiology Press, Washington, D.C
7. Gardner R., Putnam C.W., and Weinert T. RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in yeast. EMBO J. 18 (1999) 3173-3185
8. Gilbert C.S., Green C.M., and Lowndes N.F. Budding yeast Rad9 is an ATP-dependent Rad53 activating machine. Mol. Cell 8 (2001) 129-136
9. Givan A.L. "Flow Cytometry - First Principles." (1992), Wiley-Liss, New York
10. Guthrie C., and Fink G.F. "Guide to Yeast Genetics and Molecular Biology, Methods in Enzymol. 194." (1991), Academic Press, San Diego, CA
11. Kilmartin J.V., and Adams A.E.M. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J. Cell Biol. 98 (1984) 922-933
12. Lee S.-J., Duong J.K., and Stern D.F. A Ddc2-Rad53 fusion protein can bypass the requirements for RAD9 and MRC1 in Rad53 activation. Mol. Biol. Cell 15 (2004) 5443-5455
13. Lee S.-J., Schwartz M.F., Duong J.K., and Stern D.F. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Mol. Cell. Biol. 23 (2003) 6300-6314
14. Lee S.E., Moore J.K., Holmes A., Umezu K., Kolodner R.D., and Haber J.E. Saccharomyces Ku70, Mre11/Rad50, and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell 94 (1998) 399-409
15. Nyberg K.A., Michelson R.J., Putnam C.W., and Weinert T.A. Toward maintaining the genome: DNA damage and replication checkpoints. Annu. Rev. Genet. 36 (2002) 617-656
16. Paulovich A.G., and Hartwell L.H. A checkpoint regulates the rate of progression through S phase in S. cerevisiae in response to DNA damage. Cell 82 (1995) 841-847
17. Pellicioli A., Lucca C., Liberi G., Marini F., Lopes M., Plevani P., Romano A., Di Fiore P.P., and Foiani M. Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase. EMBO J. 18 (1999) 6561-6572
18. Sambrook J., Fritsch E.F., and Maniatis T. "Molecular Cloning - A Laboratory Manual." (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
19. Sanchez Y., Desany B.A., Jones W.J., Liu Q., Wang B., and Elledge S.J. Regulation of RAD53 by the ATM-like kinases MEC1 and TEL1 in yeast cell cycle checkpoint pathways. Science 271 (1996) 357-360
20. Sun Z., Fay D.S., Marini F., Foiani M., and Stern D.F. Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways. Genes Dev. 10 (1996) 395-406
21. Weinert T.A., and Hartwell L.H. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241 (1988) 317-322
22. Yamamoto A., Guacci V., and Koshland D. Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s). J. Cell Biol. 133 (1996) 99-110
23. Zhang H., and Siede W. Analysis of the budding yeast Saccharomyces cerevisiae cell cycle by morphological criteria and flow cytometry. In: Lieberman H.B. (Ed). "Cell Cycle Checkpoint Protocols" Vol. 241 (2004), Humana Press, Totowa, NJ 77-91