Cloning, purification, crystallization and preliminary crystallographic analysis of SecA from Enterococcus faecalis

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 62, Issue 6, 2006, Pages 583-585

  Meining, Winfried ^a   Scheuring, Johannes ^b   Fischer, Markus ^b   Weinkauf, Sevil ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BACTERIAL PROTEIN; CARRIER PROTEIN; SECA PROTEIN, BACTERIA; SOLVENT;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; CRYSTALLIZATION; ENTEROCOCCUS FAECALIS; ENZYMOLOGY; METHODOLOGY; MOLECULAR CLONING; X RAY DIFFRACTION;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CLONING, MOLECULAR; CRYSTALLIZATION; ENTEROCOCCUS FAECALIS; MEMBRANE TRANSPORT PROTEINS; SOLVENTS; X-RAY DIFFRACTION;

ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI;

EID: 33745127322     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309106017544     Document Type: Article

References (20)

1. Bader, G., Gomez-Ortiz, M., Haussmann, C., Bacher, A., Huber, R. & Fischer, F. (2004). Acta Cryst. D60, 1068-1075.
2. Benach, J., Chou, Y. T., Fak, J. J., Itkin, A., Nicolae, D. D., Smith, P. C., Wittrock, G., Floyd, D. L., Golsaz, C. M., Gierasch, L. M. & Hunt, J. F. (2003). J. Biol. Chem. 278, 3628-3638.
3. Ding, H., Hunt, J. F., Mukerji, I. & Oliver, D. (2003). Biochemistry, 42, 8729-8738.
4. Driessen, A. J. M. (1993). Biochemistry, 32, 13190-13197.
5. Duong, F. (2003). EMBO J. 22, 4375-4384.
6. Hunt, J. F., Weinkauf, S., Henry, L., Fak, J. J., McNicholas, P., Oliver, D. B. & Deisenhofer, J. (2002). Science, 297, 2018-2026.
7. Huycke, M. M., Sahm, D. F. & Gilmore, M. S. (1998). Emerg. Infect. Dis. 4, 239-249.
8. Kantardjieff, K. A. & Rupp, B. (2003). Protein Sci. 12, 1865-1871.
9. Keyzer, J. de, van der Does, C. & Driessen, A. J. M. (2003). Cell. Mol. Life Sci. 60, 2034-2052.
10. Keyzer, J. de, van der Sluis, E. O., Spelbrink, R. E., Njistadt, N, de Kruijff, B., Nouwen, N., van der Does, C. & Driessen, A. J. M. (2005). J. Biol. Chem. 280, 35255-35260.
11. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
12. Or, E., Boyd, D., Gon, S., Beckwith, J. & Rapoport, T. (2005). J. Biol. Chem. 280, 9097-9105.
13. Osborne, A. R., Clemons, W. M. Jr & Rapoport, T. A. (2004). Proc. Natl Acad. Sci. USA, 101, 10937-10942.
14. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
15. Read, R. J. (2001). Acta Cryst. D57, 1373-1382.
16. Sharma, V., Arockiasamy, A., Ronning, D. R., Savva, C. G., Holzenburg, A., Braunstein, M., Jacobs, W. R. Jr & Sacchettini, J. C. (2003). Proc. Natl Acad. Sci. USA, 100, 2243-2248.
17. Stueber, D., Matile, H. & Garotta, G. (1990). Immunological Methods, Vol. IV, edited by I. Lefkovits & P. Pernis, pp. 121-125. New York: Academic Press.
18. Takamatsu, H., Fuma, S., Nakamura, K., Sadaie, Y., Shinkai, A., Matsuyama, S., Mizushima, S. & Yamane, K. (1992). J. Bacteriol. 174, 4308-4316.
19. Vrontou, E. & Economou, A. (2004). Biochim. Biophys. Acta, 1694, 67-80.
20. Woodbury, R. L., Hardy, S. J. & Randall, L. L. (2002). Protein Sci. 11, 875-882.