Comparison of cell culture and RT-PCR for the detection of enterovirus in sewage and shellfish;Comparaison de la culture cellulaire et de la RT-PCR pour la détection des entérovirus dans les eaux usées et les coquillages en Tunisie

Pathologie Biologie

Volumn 54, Issue 5, 2006, Pages 280-284

  Sdiri, K ^a   Khelifi, H ^a   Belghith, K ^a   Aouni, M ^a  

^a UNIVERSITY OF MONASTIR   (Tunisia)

Author keywords

Cell culture; Enterovirus; RT PCR; Sewage; Shellfish

Indexed keywords

VIRUS RNA;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; ENTEROVIRUS; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEWAGE; SHELLFISH; STATISTICAL ANALYSIS; VIRUS CULTURE; VIRUS DETECTION; WASTE WATER; WATER SAMPLING; ANIMAL; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MOLLUSC; SENSITIVITY AND SPECIFICITY; VIROLOGY; WATER POLLUTION;

ANIMALS; ENTEROVIRUS; MOLLUSCA; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEWAGE; SHELLFISH; WATER POLLUTION;

EID: 33745007950     PISSN: 03698114     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.patbio.2005.12.001     Document Type: Article

References (20)

1. Pommepuy M. Virus et coquillages : quel risque et comment le prévenir ?. Bull. Soc. Fr. Microbiol. 16 (2001) 95-98
2. Miossec L., and Vaillant V. Épidémiologie des gastroentérites virales associées à la consommation des coquillages. Bull. Soc. Fr. Microbiol. 16 (2001) 103-114
3. Katayama H., Shimazaki A., and Ohgaki S. Development of a virus concentration method and its application to detection of enterovirus and Norwalk virus from coastal seawater. Appl. Environ. Microbiol. 68 (2002) 1033-1039
4. Chapron C.D., Ballester N.A., Fontaine J.H., Frades C.N., and Margolin A.B. Detection of astroviruses, enteroviruses and adenovirus types 40 and 41 in surface waters collected and evaluated by the information collection rule and an integrated cell culture-nested PCR procedure. Appl. Environ. Microbiol. 66 (2000) 2520-2525
5. Billaudel S., and Aubineau-Ferré V. Virus et coquillages. Lettre de l'infectiologue 5 (1999) 195-198
6. Schwartzbrod L. Virus, eaux et coquillages. In: Lesne (Ed). Coquillages et santé publique : du risque à la prévention (1992), ENSP éditeur, Rennes 35-49
7. Kopecka H., Dubrou S., Prevot J., Marechal J., and Lopez-Pila J.M. Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction and hybridization. Appl. Environ. Microbiol. 59 (1993) 1213-1219
8. Le Guyader F., Estes M.K., Kopecka H., Le Cann P., and Pommepuy M. Apport de la biologie moléculaire pour détecter les virus entériques humains dans les coquillages. Virologie 3 (2000) 241-247
9. Gantzer C., Lucena F., Schwartzbrod L., and Jofre J. Indicateurs de contamination virale du milieu hydrique : mythe ou réalité ?. Virologie 2 (1998) 117-125
10. Gerba C.P., Goyal S.M., Labelle R.L., Cech I., and Bodgan G.F. Failure of indicator bacteria to reflect the occurrence of enteroviruses in marine waters. Am. J. Public Health 69 (1979) 1116-1119
11. Kohli E., Bon F., and Pothier P. Les principaux virus de gastroentérites chez l'homme. Bull. Soc. Fr. Microbiol. 16 (2001) 97-102
12. Le Guyader F., Haugarreau L., Miossec L., Dubois E., and Pommepuy M. Three-year study to assess human enteric viruses in shellfish. Appl. Environ. Microbiol. 66 (2000) 3241-3248
13. Yang Y.T., Yam P., Yan H., and Sun Y. Engineered BGMK cells for sensitive and rapid detection of enteroviruses. J. Clin. Microbiol. 40 (2002) 366-371
14. Le Guyader F., Dubois E., and Pommepy M. Détection des virus entériques humains dans les coquillages. Bull. Soc. Fr. Microbiol. 16 (2001) 115-119
15. Trabelsi A., Grattard F., Nejmeddine M., Aouni M., Bourlet T., and Pozzetto B. Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody 5-D8/1, in comparison with neutralisation and PCR for rapid identification of enteroviruses in cell culture. J. Clin. Microbiol. 33 (1995) 2454-2457
16. Zoll G.J., Melchers W.J.G., Kopecka H., Jambroes G., van der Poel H.J., and Galama J.M. General primer mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistant infections. J. Clin. Microbiol. 30 (1992) 160-165
17. Monpoeho S., Dehee A., Mignotte B., Schartzbrod L., Marechal V., Nicolas J.-C., et al. Quantification of enterovirus RNA in sludge samples by single tube real-time RT-PCR. Biotechniques 29 (2000) 88-93
18. Metcalf T.G., Melnick J.L., and Estes M.K. Environmental virology: from detection of virus in sewage and water by isolation to identification by molecular biology. A trip of over 50 years. Annu. Rev. Microbiol. 49 (1995) 461-487
19. Chung H., Jaykus L.A., and Sobsey M.D. Detection of human enteric viruses in oysters by in vivo and in vitro amplification of nucleic acids. Appl. Environ. Microbiol. 62 (1996) 3772-3778
20. Monpoeho S., Maul A., Mignotte-Cadiergues B., Schwartzbrod L., Billaudel S., and Ferre V. Best viral elution method available for quantification of enteroviruses in sludge by both cell culture and reverse transcription-PCR. Appl. Environ. Microbiol. 67 (2001) 2484-2488