Intestinal morphology and enzymatic activity in newly weaned pigs fed contrasting fiber concentrations and fiber properties

Journal of Animal Science

Volumn 84, Issue 6, 2006, Pages 1375-1386

  Hedemann, M S ^a   Eskildsen, M ^a   Laerke H N ^a   Pedersen, C ^b,d   Lindberg, J E ^b   Laurinen, P ^c   Knudsen, K E Bach ^a  

^a DANISH INSTITUTE OF AGRICULTURAL SCIENCES   (Denmark)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^c ANIMAL PRODUCTION RESEARCH   (Finland)

^d SOUTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

Digestive enzyme; Fiber; Gut morphology; Mucin; Pig

Indexed keywords

BACTERIA (MICROORGANISMS); HORDEUM VULGARE SUBSP. VULGARE; SUS SCROFA; TRITICUM AESTIVUM;

EID: 33744944559     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/2006.8461375x     Document Type: Article

References (49)

1. AOAC. 1990. Official Methods of Analysis. 15th ed. Assoc. Off. Anal. Chem., Arlington, VA.
2. Bach Knudsen, K. E. 1997. Carbohydrate and lignin contents of plant materials used in animal feeding. Anim. Feed Sci. Technol. 67:319-338.
3. Bach Knudsen, K. E., B. B. Jensen, J. O. Andersen, and I. Hansen. 1991. Gastrointestinal implications in pigs of wheat and oat fractions. 2. Microbial activity in the gastrointestinal-tract. Br. J. Nutr. 65:233-248.
4. Brown, P. J., B. G. Miller, C. R. Stokes, N. B. Blazquez, and F. J. Bourne. 1988. Histochemistry of mucins of pig intestinal secretory epithelial cells before and after weaning. J. Comp. Path. 98:313-323.
5. Brunsgaard, G. 1997. Morphological characteristics, epithelial cell proliferation, and crypt fission in cecum and colon of growing pigs. Dig. Dis. Sci. 42:2384-2393.
6. Brunsgaard, G. 1998. Effects of cereal type and feed particle size on morphological characteristics, epithelial cell proliferation, and lectin binding patterns in the large intestine of pigs. J. Anim. Sci. 76:2787-2798.
7. Brunsgaard, G., and B. O. Eggum. 1995. Small intestinal tissue structure and proliferation as influenced by adaptation period and indigestible polysaccharides. Comp. Biochem. Physiol. 112A:365-377.
8. Burrin, D. G., B. Stoll, R. Jiang, Y. Petersen, J. Elnif, R. K. Buddington, M. Schmidt, J. J. Holst, B. Hartmann, and P. T. Sangild. 2000. GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis. Am. J. Physiol. 279:G1249-G1256.
9. Callesen, J. 2004. Effects of termination of AGP-use on pig welfare and productivity. Pages 57-60 in Working Papers from the International Symposium: Beyond Antimicrobial Growth Promoters in Food Animal Production. DIAS report Animal Husbandry no. 57, Tjele, Denmark.
10. Chun, W., T. Bamba, and S. Hosoda. 1989. Effect of pectin, a soluble dietary fiber, on functional and morphological parameters of the small-intestine in rats. Digestion 42:22-29.
11. Dahlqvist, A. 1968. Assay of intestinal disaccharidases. Anal. Biochem. 22:99-107.
12. Deplancke, B., and H. R. Gaskins. 2001. Microbial modulation of innate defense: Goblet cells and the intestinal mucus layer. Am. J. Clin. Nutr. 73:11318-11418.
13. Freire, J. P. B., A. J. G. Guerreiro, L. F. Cunha, and A. Aumaitre. 2000. Effect of dietary fibre source on total tract digestibility, caecum volatile fatty acids and digestive transit time in the weaned piglet. Anim. Feed Sci. Technol. 87:71-83.
14. Fukunaga, T., M. Sasaki, Y. Araki, T. Okamoto, T. Yasuoka, T. Tsujikawa, Y. Fujiyama, and T. Bamba. 2003. Effects of the soluble fibre pectin on intestinal cell proliferation, fecal short chain fatty acid production and microbial population. Digestion 67:42-49.
15. Gibson, G. R., and X. Wang. 1994. Regulatory effects of bifidobacteria on the growth of other colonic bacteria. J. Appl. Bacteriol. 77:412-420.
16. Glitsø, L. V., G. Brunsgaard, S. Højsgaard, B. Sandstrøm, and K. E. Bach Knudsen. 1998. Intestinal degradation in pigs of rye dietary fibre with different structural characteristics. Br. J. Nutr. 80:457-468.
17. Goodlad, R. A. 1994. Microdissection-based techniques for the detection of cell proliferation in gastrointestinal epithelium: Application to animal and human studies. Pages 205-216 in Cell Biology: A Laboratory Handbook. Academic Press Inc., San Diego, CA.
18. Hampson, D. J., and D. E. Kidder. 1986. Influence of creep feeding and weaning on brush border enzyme activities in the piglet small intestine. Res. Vet. Sci. 40:24-31.
19. Hedemann, M. S., S. Højsgaard, and B. B. Jensen. 2003. Small intestinal morphology and activity of intestinal peptidases in piglets around weaning. J. Anim. Physiol. Anim. Nutr. 87:32-41.
20. Hedemann, M. S., L. L. Mikkelsen, P. J. Naughton, and B. B. Jensen. 2005. Effect of feed particle size and feed processing on morphological characteristics in the small and large intestine of pigs and on adhesion of Salmonella enterica serovar Typhimurium DT12 in the ileum in vitro. J. Anim. Sci. 83:1554-1562.
21. Iji, P. A., A. A. Saki, and D. R. Tivey. 2001. Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Anim. Feed Sci. Technol. 89:175-188.
22. Jaeger, L. A., C. H. Lamar, and J. J. Turek. 1989. Lectin binding to small intestinal goblet cells of newborn, suckling, and weaned pigs. Am. J. Vet. Res. 50:1984-1987.
23. Jensen, B. B. 1998. The impact of feed additives on the microbial ecology of the gut in young pigs. J. Anim. Feed Sci. 7:45-64.
24. Jin, L., L. P. Reynolds, D. A. Redmer, J. S. Caton, and J. D. Crenshaw. 1994. Effects of dietary fiber on intestinal growth, cell proliferation, and morphology in growing pigs. J. Anim. Sci. 72:2270-2278.
25. Johansen, H. N., K. E. Bach Knudsen, P. J. Wood, and R. G. Fulcher. 1997. Physico-chemical properties and the degradation of oat bran polysaccharides in the gut of pigs. J. Sci. Food Agric. 73:81-92.
26. Kiernan, J. A. 1990. Histological and Histochemical Methods. Theory and Practice. Pergamon Press, Oxford, UK.
27. Kim, M. 2002. The water-soluble extract of chicory affects rat intestinal morphology similarly to other non-starch polysaccharides. Nutr. Res. 22:1299-1307.
28. Koninkx, J. F., A. F. Stemerdink, M. H. Mirck, H. J. Egberts, J. E. van Dijk, and J. M. Mouwen. 1988. Histochemical changes in the composition of mucins in goblet cells during methotrexate-induced mucosal atrophy in rats. Exp. Pathol. 34:125-132.
29. Larsson, K., and S. Bengtsson. 1983. Bestämning av lättilgängelig kolhydrater i växtmaterial (Determination of readily available carbohydrates in plant material). Chemistry Methods Report No. 22. Natl. Lab. Agric. Chem., Uppsala, Sweden.
30. Littell, R. C., G. A. Milliken, W. W. Stroup, and R. D. Wolfinger. 1996. SAS System for Mixed Models. SAS Inst. Inc., Cary, NC.
31. Lizardo, R., J. Peiniau, and A. Aumaitre. 1997. Inclusion of sugar-beet pulp and change of protein source in the diet of the weaned piglet and their effects on digestive performance and enzymatic activities. Anim. Feed Sci. Technol. 66:1-14.
32. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265-275.
33. Marion, J., M. Biernat, F. Thomas, G. Savary, Y. Le Breton, R. Zabielski, I. Huerou-Luron, and J. Le Dividich. 2002. Small intestine growth and morphometry in piglets weaned at 7 days of age. Effects of level of energy intake. Reprod. Nutr. Dev. 42:339-354.
34. McDonald, D. E., D. W. Pethick, B. P. Mullan, and D. J. Hampson. 2001. Increasing viscosity of the intestinal contents alters small intestinal structure and intestinal growth, and stimulates proliferation of enterotoxigenic Escherichia coli in newly-weaned pigs. Br. J. Nutr. 86:487-498.
35. Montagne, L., J. R. Pluske, and D. J. Hampson. 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Technol. 108:95-117.
36. More, J., J. Fioramonti, F. Benazet, and L. Bueno. 1987. Histochemical characterization of glycoproteins present in jejunal and colonic goblet cells of pigs on different diets. A biopsy study using chemical methods and peroxidase-labelled lectins. Histochemistry 87:189-194.
37. NCPP. 2005. Nutrients Standards. National Committee for Pig Production, Copenhagen, Denmark. Available: http://www.danskes-lagterier.dk/view.asp?ID=1753 Accessed Jan. 11, 2006.
38. Neutra, M. R., and J. F. Forstner. 1987. Gastrointestinal mucus: Synthesis, secretion, and function. Pages 975-1009 in Physiology of the Gastrointestinal Tract. L. R. Johnson, ed. Raven Press, New York, NY.
39. Nyachoti, C. M., F. O. Omogbenigun, M. Rademacher, and G. Blank. 2006. Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. J. Anim. Sci. 84:125-134.
40. Pluske, J. R. 2001. Morphological and functional changes in the small intestine of the newly-weaned pig. Pages 1-27 in A. Piva, K. E. Bach Knudsen, and J. E. Lindberg, ed. Gut Environment of Pigs. Nottingham Univ. Press, Nottingham, UK.
41. Pluske, J. R., D. J. Hampson, and I. H. Williams. 1997. Factors influencing the structure and function of the small intestine in the weaned pig: A review. Livest. Prod. Sci. 51:215-236.
42. Pluske, J. R., I. H. Williams, and F. X. Aherne. 1996. Maintenance of villous height and crypt depth in piglets by providing continous nutrition after weaning. Anim. Sci. 62:131-144.
43. Spreeuwenberg, M. A. M., J. M. A. J. Verdonk, H. R. Gaskins, and M. W. A. Verstegen. 2001. Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J. Nutr. 131:1520-1527.
44. Stoldt, W. 1952. Vorschlag zur Vereinheitlichung der Fettbestimmung in Lebensmittlen. F. Seif. Anst. 54:146-164.
45. Tasman-Jones, C., R. L. Owen, and A. L. Jones. 1982. Semipurified dietary fiber and small-bowel morphology in rats. Dig. Dis. Sci. 27:519-524.
46. Theander, O., and P. Aman. 1979. Studies on dietary fibres. Swed. J. Agr. Res. 9:97-106.
47. van Beers-Schreurs, H. M. G., M. J. A. Nabuurs, L. Vellenga, H. J. Kalsbeek-van der Valk, T. Wensing, and H. J. Breukink. 1998. Weaning and the weanling diet influence the villous height and crypt depth in the small intestine of pigs and alter the concentrations of short-chain fatty acids in the large intestine and blood. J. Nutr. 128:947-953.
48. Vente-Spreeuwenberg, M. A. M., J. M. A. J. Verdonk, A. C. Beynen, and M. W. A. Verstegen. 2003. Interrelationships between gut morphology and faeces consistency in newly weaned piglets. Anim. Sci. 77:85-94.
49. Wang, X., and G. R. Gibson. 1993. Effects of the in-vitro fermentation of oligofructose and inulin by bacteria growing in the human large-intestine. J. Appl. Bacteriol. 75:373-380.