AFM observations of (DMPC/cholesterol) mixed monolayer on aqueous solution of Vibrio cholerae hemolysin

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Volumn 284-285, Issue , 2006, Pages 475-479

  Tagami, Yasunobu ^a   Ikigai, Hajime ^b   Oishi, Yushi ^a  

^a SAGA UNIVERSITY   (Japan)

^b SUZUKA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Atomic force microscopy; Cholesterol; Monolayer; Phospholipid; Vibrio cholerae hemolysin

Indexed keywords

ATOMIC FORCE MICROSCOPY; CHOLESTEROL; ISOTHERMS; MICROSCOPIC EXAMINATION; PHOSPHOLIPIDS; SOLUTIONS;

CHOLESTEROL-RICH PHASE; ISOTHERM MEASUREMENTS; VIBRIO CHOLERAE HEMOLYSIN;

MONOLAYERS;

CHOLESTEROL; DIMYRISTOYLPHOSPHATIDYLCHOLINE; HEMOLYSIN; PHOSPHOLIPID;

AQUEOUS SOLUTION; ARTICLE; ARTIFICIAL MEMBRANE; ATOMIC FORCE MICROSCOPY; CONCENTRATION RESPONSE; MONOLAYER CULTURE; PRIORITY JOURNAL; SURFACE PROPERTY; TEMPERATURE SENSITIVITY; VIBRIO CHOLERAE;

EID: 33744919562     PISSN: 09277757     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.colsurfa.2005.11.009     Document Type: Article

References (21)

1. Levine M.M., Kaper J.B., Herrington D., Losonsky G., Morris J.G., Clements M.L., Black R.E., Tall B., and Hall R. Infect. Immun. 56 (1988) 161
2. Ikigai H., Akatsuka A., Tsujiyama H., Nakae T., and Shimamura T. Infect. Immun. 64 (1996) 2968
3. Campbell M.K., and Farrell S.O. Biochemistry (2003), Thomson Learning Inc.
4. Overney R.M., Meyer E., Frommer J., Brodbeck D., Lüthi R., Howald L., Güntherodt H.J., Fujihira M., Takano H., and Gotoh Y. Nature 359 (1992) 133
5. Chi L.F., Anders M., Fuchi H., Johnston R.R., and Ringsdorf H. Science 259 (1993)
6. Takahara A., Kojio K., Ge S., and Kajiyama T. J. Vac. Sci. Technol. A14 (1996) 1747
7. Iimura K., and Kato T. Colloid. Surf. 143 (1998) 491
8. Oishi Y., Arima H., Suehiro K., and Era M. Thin Solid Films 44 (2003) 438
9. The (DMPC/cholesterol) mixed monolayer with a molar fraction of 70/30 on water had been characterized to be in a phase-separated state by a fluorescence microscope. There is a difference in content of cholesterol (also DMPC) between domain and matrix regions in the monolayer. The molayer observation on aqueous VCH monolayer. The monolayer observation on aqueous VCH may offer the key to specify which component of the monolayer is responsible for binding with VCH. Then, we adopted the molar fraction of 70/30 as the condition of monolayer preparation.
10. The π-A isotherm of the mixed monolayer on aqueous VCH solution was measured by changing the standing time up to 90 min. The isotherm slightly shifted to a larger surface area even at 90 min. This indicates that more than 90 min is required for the adsorption saturation of VCH. However, it is difficult to measure the isotherm for long time because of water evaporation in subphase [T. Kuri, et al., Langmuir 13 (1997), 6497]. Then, we adopted 30 min as the adsorption time of VCH in this study.
11. Oishi Y., Kuri T., Takashima Y., and Kajiyama T. Chem. Lett. (1994) 1445
12. Schaper A., Wolthaus L., Möbius D., and Jovin T.M. Langmuir 9 (1993) 2178
13. McConnell H.M., and Moy V.T. J. Phys. Chem. 92 (1988) 4520
14. Iimura K., Siraku T., and Kato T. Langmuir 18 (2002) 10183
15. The color contrast of the magnified AFM image was emphasized in comparison with the height difference. The average surface roughness of the magnified AFM image was 0.11 nm.
16. Tanaka K., Manning P.A., Lau V.K., and Yu H. Langmuir 15 (1999) 600
17. Worthman L.D., Nag K., Davis P.J., and Keough K.M.W. Biophys. J. 72 (1997) 2569
18. Hirshfeld C.L., and Seul M. J. Phys. France 51 (1990) 1537
19. Kubo I., Adachi S., Maeda H., and Seki A. Thin Solid Films 80 (2001) 393
20. Heckl W.M., Cadenhead D.A., and Möhwald H. Langmuir 4 (1988) 1352
21. Molecular weight of VCH was estimated to be 65 kDa by SDS-polyacrylamide gel electrophoresis. The dot in a matrix region may correspond to an aggregate, composed of 4 or 5 subunits, of VCH.