Importin-α-16 is a translocon-associated protein involved in sorting membrane proteins to the nuclear envelope

Nature Structural and Molecular Biology

Volumn 13, Issue 6, 2006, Pages 500-508

  Saksena, Suraj ^a   Summers, Max D ^a,b   Burks, Jared K ^a   Johnson, Arthur E ^a,c   Braunagel, Sharon C ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

^c TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; KARYOPHERIN ALPHA; MEMBRANE PROTEIN; TRANSLOCON;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS MEMBRANE; CROSS LINKING; ENDOPLASMIC RETICULUM; LEPIDOPTERA; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF;

ALPHA KARYOPHERINS; AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; EPITOPES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; NUCLEAR ENVELOPE; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ARMADILLO; SPODOPTERA FRUGIPERDA;

EID: 33744915536     PISSN: 15459993     EISSN: 15459985     Source Type: Journal    
DOI: 10.1038/nsmb1098     Document Type: Article

References (37)

1. Hetzer, M., Walther, T.C. & Mattaj, I.W. Pushing the envelope: structure, function and dynamics of the nuclear periphery. Annu. Rev. Cell Dev. Biol. 21, 347-380 (2005).
2. Saksena, S., Shao, Y., Braunagel, S.C., Summers, M.D. & Johnson, A.E. Cotranslational integration and initial sorting at the endoplasmic reticulum translocon of protein destined for the inner nuclear membrane. Proc. Natl Acad. Sci. USA 101, 12537-12542 (2004).
3. Braunagel, S.C. et al. Trafficking of ODV-E66 is mediated via a sorting motif and other viral proteins: facilitated trafficking to the inner nuclear membrane. Proc. Natl Acad. Sci. USA 101, 8372-8377 (2004).
4. Ohba, T., Schirmer, E.C., Nishimoto, T. & Gerace, L. Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore. J. Cell Biol. 167, 1051-1062 (2004).
5. Hong, T., Summers, M.D. & Braunagel, S.C. N-terminal sequences from Autographa californica nuclear polyhedrosis virus envelope proteins ODV-E66 and ODV-E25 are sufficient to direct reporter proteins to the nuclear envelope, intranuclear microvesicles and the envelope of the occlusion-derived virus. Proc. Natl Acad. Sci. USA 94, 4050-4055 (1997).
6. Rosas-Acosta, G., Braunagel, S.C. & Summers, M.D. Effects of deletion and overexpression of the Autographa californica nuclear polyhedrosis virus FP25K gene on synthesis of two occlusion-derived virus envelope proteins and their transport into virus-induced intranuclear membranes. J. Virol. 75, 10829-10842 (2001).
7. Crowley, K.S., Liao, S., Worrell, V.E., Reinhart, G.D. & Johnson, A.E. Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore. Cell 78, 461-471 (1994).
8. Crowley, K.S., Reinhart, G.D. & Johnson, A.E. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation. Cell 73, 1101-1115 (1993).
9. Lee, J.M. et al. Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 139, 35-43 (2004).
10. Altschul, S.F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402 (1997).
11. Goldfarb, D.S., Corbett, A.M., Mason, D.A., Harreman, M.T. & Adam, S.A. Importin α: a multipurpose nuclear-transport receptor. Trends Cell Biol. 14, 505-514 (2004).
12. Miyamoto, Y. et al. Importin α can migrate into the nucleus in an importin β- and Ran-independent manner. EMBO J. 21, 5833-5842 (2002).
13. McCormick, P.J., Miao, Y., Shao, Y., Lin, J. & Johnson, A.E. Cotranslational protein integration into the ER membrane is mediated by the binding of nascent chains to translocon proteins. Mol. Cell 12, 329-341 (2003).
14. Jakel, S., Mingot, J.-M., Schwarzmaier, P., Hartmann, E. & Görlich, D. Importins fulfill a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains. EMBO J. 21, 377-386 (2002).
15. Chook, Y.M. & Blobel, G. Karyopherins and nuclear import. Curr. Opin. Struct. Biol. 11, 703-715 (2001).
16. Harel, A. & Forbes, D.J. Importin beta: conducting a much larger cellular symphony. Mol. Cell 16, 319-330 (2004).
17. Kotera, I. et al. Importin α transports CaMKIV to the nucleus without utilizing importin β. EMBO J. 24, 942-951 (2005).
18. Kamata, M., Nitahara-Kasahara, Y., Miyamoto, Y., Yoneda, Y. & Aida, Y. Importin-α promotes passage through the nuclear pore complex of human immunodeficiency virus Type 1 Vpr. J. Virol. 79, 3557-3564 (2005).
19. Pemberton, L.F., Rosenblum, J.S. & Blobel, G. Nuclear import of the TATA-binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting. J. Cell Biol. 145, 1407-1417 (1999).
20. Belanger, K.D., Kenna, M.A., Wei, S. & Davis, L.I. Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. J. Cell Biol. 126, 619-630 (1994).
21. Solsbacher, J., Maurer, P., Vogel, F. & Schlenstedt, G. Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin α. Mol. Cell. Biol. 20, 8468-8479 (2000).
22. Moroianu, J., Blobel, G. & Radu, A. RanGTP-mediated nuclear export of karyopherin α involves its interaction with the nucleoporin Nup153. Proc. Natl Acad. Sci. USA 94, 9699-9704 (1997).
23. Lindsay, M.E., Plafker, K., Smith, A.E., Clurman, B.E. & Macara, I.G. Npap60/Nup50 is a tri-stable switch that stimulates importin-α:β- mediated nuclear protein import. Cell 110, 349-360 (2002).
24. Moore, M.S. Npap60: a new player in nuclear protein import. Trends Cell Biol. 13, 61-64 (2003).
25. Matsuura, Y. & Stewart, M. Nup50/Npap60 function in nuclear import complex disassembly and importin recycling. EMBO J. 24, 3681-3689 (2005).
26. Hanz, S. & Fainzilber, M. Integration of retrograde axonal and nuclear transport mechanisms in neurons: implications for therapeutics. Neuroscientist 10, 404-408 (2004).
27. Hanz, S. et al. Axoplasmic importins enable retrograde inujry signaling in lesioned nerve. Neuron 40, 1095-1104 (2003).
28. Blesch, A. & Tuszynski, M.H. Nucleus hears axon's pain. Nat. Med. 10, 236-237 (2004).
29. Machet, V., Köcher, T., Wilm, T. & Mattaj, I.W. Importin α associates with membranes and participates in nuclear envelope assembly in vitro. EMBO J. 23, 1526-1535 (2004).
30. Marzalek, J.R. et al. Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian receptors. Cell 102, 175-187 (2000).
31. Williams, D.S. Transport to the photoreceptor outer segment by myosin Vila and kinesin II. Vision Res. 42, 455-462 (2002).
32. Schatz, C.A. et al. Importin α-regulated nucleation of microtubules by TPX2. EMBO J. 22, 2060-2070 (2003).
33. Gruss, O.J. et al. Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity. Cell 104, 83-93 (2001).
34. Beniya, H., Braunagel, S.C. & Summers, M.D. Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes. Virology 240, 64-75 (1998).
35. Worman, H.J. & Courvalin, J.-C. Nuclear envelope, nuclear lamina, and inherited disease. Int. Rev. Cytol. 246, 231-279 (2005).
36. Braunagel, S.C., Elton, D.M., Ma, H. & Summers, M.D. Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope, ODV-E56. Virology 217, 97-110 (1996).
37. Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 1989).