Molecular identification and characterisation of the glycine transporter (GLYT1) and the glutamine/glutamate transporter (ASCT2) in the rat lens

Experimental Eye Research

Volumn 83, Issue 2, 2006, Pages 447-455

  Lim, Julie ^a   Lorentzen, Karen A ^a   Kistler, Joerg ^b   Donaldson, Paul J ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

cyst(e)ine transport; glutamine transport; glutathione; glycine transport

Indexed keywords

ANTIOXIDANT; CARRIER PROTEIN; GLUTAMINE; GLUTAMINE GLUTAMATE TRANSPORTER PROTEIN; GLUTATHIONE; GLYCINE; GLYCINE TRANSPORTER 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOPLASM; GLUTATHIONE METABOLISM; IMMUNOCYTOCHEMISTRY; LENS; LENS EPITHELIUM; LENS FIBER; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

AMINO ACID TRANSPORT SYSTEM ASC; ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELL MEMBRANE; CYSTEINE; CYTOPLASM; EYE PROTEINS; GLUTAMINE; GLUTATHIONE; GLYCINE; GLYCINE PLASMA MEMBRANE TRANSPORT PROTEINS; IMMUNOHISTOCHEMISTRY; LENS CORTEX, CRYSTALLINE; LENS, CRYSTALLINE; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 33744914329     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2006.01.028     Document Type: Article

References (30)

1. Aragon C., and Lopez-Corcuera B. Structure, function and regulation of glycine neurotransporters. Eur. J. Pharmacol. 479 (2003) 249-262
2. Avissar N.E., Ryan C.K., Ganapathy V., and Sax H.C. Na(+)-dependent neutral amino acid transporter ATB(0) is a rabbit epithelial cell brush-border protein. Am. J. Physiol. Cell Physiol. 281 (2001) C963-C971
3. Baldo G.J., and Mathias R.T. Spatial variations in membrane properties in the intact lens. Biophys. J. 63 (1992) 518-529
4. Bode B. Recent molecular advances in mammalian glutamine transport. J. Nutr. 131 (2001) 2475S-24785S
5. Bungard C.I., and McGivan J.D. Glutamine availability up-regulates expression of the amino acid transporter protein ASCT2 in HepG2 cells and stimulates the ASCT2 promoter. Biochem. J. 382 (2004) 27-32
6. Chee, K.N., Kistler, J., Donaldson, P.J., 2006. Roles for KCC transporters in the maintenance of lens transparency. Invest. Ophthalmol. Vis. Sci. 47, 673-682.
7. Dickerson Jr. J.E., and Lou M.F. Free cysteine in normal human lenses. Exp. Eye Res. 65 (1997) 451-454
8. Donaldson P.J., Grey A.C., Merriman-Smith B.R., Sisley A.M., Soeller C., Cannell M.B., and Jacobs M.D. Functional imaging: new views on lens structure and function. Clin. Exp. Pharmacol. Physiol. 31 (2004) 890-895
9. Giblin F.J. Glutathione: a vital lens antioxidant. J. Ocul. Pharmacol. Ther. 16 (2000) 121-135
10. Jacobs M.D., Soeller C., Sisley A.M., Cannell M.B., and Donaldson P.J. Gap junction processing and redistribution revealed by quantitative optical measurements of connexin46 epitopes in the lens. Invest. Ophthalmol. Vis. Sci. 45 (2004) 191-199
11. Jernigan H.M.J., and Zigler J.S.J. Metabolism of glutamine and glutamate in monkey lens. Exp. Eye Res. 44 (1987) 871-876
12. Kannan R., Yi J.R., Tang D., Zlokovic B.V., and Kaplowitz N. Identification of a novel, sodium-dependent, reduced glutathione transporter in the rat lens epithelium. Invest. Ophthalmol. Vis. Sci. 37 (1996) 2269-2275
13. Kannan R., Yi J.R., Zlokovic B.V., and Kaplowitz N. Molecular characterization of a reduced glutathione transporter in the lens. Invest. Ophthalmol. Vis. Sci. 36 (1995) 1785-1792
14. Kekuda R., Prasad P.D., Fei Y.J., Torres-Zamorano V., Sinha S., Yang-Feng T.L., Leibach F.H., and Ganapathy V. Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line. J. Biol. Chem. 271 (1996) 18657-18661
15. Li L., Lee T.K., and Ballatori N. Functional re-evaluation of the putative glutathione transporters, RcGshT and RsGshT. Yale J. Biol. Med. 70 (1997) 301-310
16. Lim J.C., Lam Y.C., Kistler J., and Donaldson P.J. Molecular characterization of the cystine/glutamate exchanger and the excitatory amino acid transporters in the rat lens. Invest. Ophthalmol. Vis. Sci. 46 (2005) 2869-2877
17. Lopez-Corcuera B., Martinez-Maza R., Nunez E., Roux M., Supplisson S., and Aragon C. Differential properties of two stably expressed brain-specific glycine transporters. J. Neurochem. 71 (1998) 2211-2219
18. Lou M.F. Redox regulation in the lens. Prog. Retin. Eye Res. 22 (2003) 657-682
19. Mackic J.B., Jinagouda S., McComb J.G., Weiss M.H., Kannan R., Kaplowitz N., and Zlokovic B.V. Transport of circulating reduced glutathione at the basolateral side of the anterior lens epithelium: physiologic importance and manipulations. Exp. Eye Res. 62 (1996) 29-37
20. Mackic J.B., Kannan R., Kaplowitz N., and Zlokovic B.V. Low de novo glutathione synthesis from circulating sulfur amino acids in the lens epithelium. Exp. Eye Res. 64 (1997) 615-626
21. Merriman-Smith B.R., Donaldson P.J., and Kistler J. Differential expression of facilitative glucose transporters GLUT1 and GLUT3 in the lens. Invest. Ophthalmol. Vis. Sci. 40 (1999) 3224-3230
22. Nelson N., and Lill H. Porters and neurotransmitter transporters. J. Exp. Biol. 196 (1994) 213-228
23. Reddy V.N. Glutathione and its function in the lens-an overview. Exp. Eye Res. 50 (1990) 771-778
24. Sato H., Tamba M., Ishii T., and Bannai S. Cloning and expression of a plasma membrane cystine/glutamate exchange transporter composed of two distinct proteins. J. Biol. Chem. 247 (1999) 11455-11458
25. Stewart-DeHaan P.J., Dzialoszynski T., and Trevithick J.R. Modelling cortical cataractogenesis XXIV: uptake by the lens of glutathione injected into the rat. Mol. Vis. 5 (1999) 37
26. Sweeney M.H., and Truscott R.J. An impediment to glutathione diffusion in older normal human lenses: a possible precondition for nuclear cataract. Exp. Eye Res. 67 (1998) 587-595
27. +-dependent neutral amino acid transporter. J. Biol. Chem. 271 (1996) 14883-14890
28. Veltman J., and Lou M.F. Quantification of free cysteine in the lens. Invest. Ophthalmol. Vis. Sci. 34 Suppl. (1993) 758
29. Yamamoto T., Nishizaki I., Nukada T., Kamegaya E., Furuya S., Hirabayashi Y., Ikeda K., Hata H., Kobayashi H., Sora I., and Yamamoto H. Functional identification of ASCT1 neutral amino acid transporter as the predominant system for the uptake of L-serine in rat neurons in primary culture. Neurosci. Res. 49 (2004) 101-111
30. Zafra F., Aragon C., Olivares L., Danbolt N.C., Gimenez C., and Storm-Mathisen J. Glycine transporters are differentially expressed among CNS cells. J. Neurosci. 15 (1995) 3952-3969