Site-specific DNA photocleavage by rhodium intercalators analyzed by MALDI-TOF mass spectrometry

Journal of the American Chemical Society

Volumn 128, Issue 21, 2006, Pages 6772-6773

  Brunner, Jens ^a   Barton, Jacqueline K ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; FURANONE DERIVATIVE; INTERCALATING AGENT; LACTONE DERIVATIVE; NUCLEOTIDE; OLIGOMER; PHOSPHATE; RHODIUM;

ARTICLE; BINDING SITE; CALIBRATION; CHEMICAL STRUCTURE; DNA CLEAVAGE; DNA DAMAGE; DNA STRAND; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; OXIDATION; REACTION ANALYSIS; STRUCTURE ANALYSIS; SYNTHESIS;

DNA; INTERCALATING AGENTS; PHOTOCHEMISTRY; RHODIUM; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 33744829513     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0612753     Document Type: Article

References (20)

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14. Photocleavage conditions: 2 μM, 2 μM mismatched DNA (for the sequence, see Figure 2), 50 mM NaCl, 10 mM NaPi pH 7, 15 min irradiation with a 1000 W Oriel Hg/Xe are lamp and light of 320-440 nm wavelength.
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17. This product was proposed as an intermediate in the copper phenanthroline-mediated oxidative DNA cleavage. See: Chen, T.; Greenberg, M. M. J. Am. Chem. Soc. 1998, 120, 3815-3816.
18. Degradation of metastable cleavage products over time was also observed by others. See: Kuwabara, M.; Yoon, C.; Goyne, T.; Thederahn, T.; Sigman, D. S. Biochemistry 1986, 25, 7401-7408.
19. This higher cleavage yield as well as cleavage position with the unmodified complex is consistent with observations obtained by quantitative gel electrophoresis analysis of the photocleavage reaction.
20. The products 4 and 8 as assigned would suggest that the phosphodiester backbone is cleaved prior to water attack.