A high-performance liquid chromatography method for hypoxanthine determination in vitreous humour: Application to estimation of post mortem interval

Human and Experimental Toxicology

Volumn 25, Issue 5, 2006, Pages 279-281

  Muñoz, José Ignacius ^a,b   Costas, E ^a   Rodriguez Calvo M S ^a   Suarez Penaranda J M ^a   Lopez Rivadulla M ^a   Concheiro, L ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

HPLC; Hypoxanthine; Post mortem interval; Regression line; Vitreous humour

Indexed keywords

HYPOXANTHINE;

ARTICLE; AUTOPSY; CONTROLLED STUDY; FORENSIC SCIENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; PRIORITY JOURNAL; REGRESSION ANALYSIS; REPRODUCIBILITY; VITREOUS BODY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FORENSIC MEDICINE; HUMANS; HYPOXANTHINES; POSTMORTEM CHANGES; REPRODUCIBILITY OF RESULTS; VITREOUS BODY;

EID: 33744825992     PISSN: 09603271     EISSN: None     Source Type: Journal    
DOI: 10.1191/0960327106ht615oa     Document Type: Article

References (10)

1. Bocaz-Beneventi G, Tagliari F, Bortolotti F, Manetto G, Havel J. Capillary zone electrophoresis and artificial neural networks for estimation of the post-mortem interval (PMI) using electrolytes measurements in human vitreous humour. Int J Legal Med 2002; 116: 5-11.
2. Schafer AT. Colour measurements of pallor mortis. Int J Legal Med 2000; 113: 81-83.
3. Muñoz JI, Suárez-Penaranda JM, Otero XL, Rodríguez-Calvo MS, Costas E, Miguéns X, Concheiro L. Improved estimation of postmortem interval based on differential behaviour of vitreous potassium and hypoxanthine in death by hanging. Forensic Sci Int 2002; 125: 67-74.
4. Madea B, Käferstein H, Hermann N, Sticht G. Hypoxanthine in vitreous humour and cerebrospinal fluid - a marker of postmortem interval and prolonged (vital) hypoxia? Remarks also on hypoxanthine in SIDS. Forensic Sci Int 1994; 65: 19-31.
5. Rognum TO, Hauge S, Oyasaeter S, Saugstad OD. A new biochemical method for estimation of postmortem time. Forensic Sci Int 1991; 51: 139-46.
6. James RA, Hoadley PA, Sampson BG. Determination of postmortem interval by sampling vitreous humour. Am J Forensic Med Pathol 1997; 18: 158-62.
7. Muñoz JI, Suárez-Peñaranda JM, Otero XL, Rodríguez-Calvo MS, Costas E, Miguéns X, Concheiro L. A new perspective in the estimation of postmortem interval (PMI) based on vitreous [K +]. J Forensic Sci Int 2001; 46: 209-14.
8. Wung WE, Howell SB. Simultaneous liquid chromatography of 5-fluorouracil, uridine, hypoxanthine, xanthine, uric acid, allopurinol, and oxipurinol in plasma. Clin Chem 1980; 26: 1704-708.
9. Simmonds RJ, Harkness RA. High-performance liquid Chromatographic methods for base and nucleoside analysis in extracellular fluids and in cells. J Chromatogr 1981; 226: 369-81.
10. Hansson L, Uotila U, Lindfors R, Laiho K. Potassium content of the vitreous body as an aid in determining the time of death. J Forensic Sci 1966; 11: 390-94.