Effect of the cryopreservation conditions on the viability of the rumen ciliate Diploplastron (Metadinium) affine

Letters in Applied Microbiology

Volumn 42, Issue 6, 2006, Pages 573-577

  De La Fuente, G ^a   Cebrian J A ^a   Fondevila, M ^a  

^a UNIVERSITY OF ZARAGOZA   (Spain)

Author keywords

Cryopreservation; Diploplastron affine; Rumen protozoa; Viability

Indexed keywords

BIOLOGICAL MATERIALS PRESERVATION; COOLING; GLYCEROL; MOLECULAR BIOLOGY; ORGANIC SOLVENTS; PROTOZOA; THAWING;

CONDITION; COOLING RATES; CRYO-PRESERVATION; CRYOPRESERVATION CONDITIONS; DIPLOPLASTRON AFFINE; EQUILIBRIUM TEMPERATURES; RUMEN PROTOZOA; VIABILITY; VIABLE CELLS;

DIMETHYL SULFOXIDE;

DIMETHYL SULFOXIDE; GLYCEROL;

BIOLOGICAL MATERIALS PRESERVATION; BIOLOGICAL MEMBRANES; CELLS; GLYCEROL; MICROBIOLOGY; PROTOZOA; THERMAL EFFECTS;

CILIATE; COOLING; CRYOPRESERVATION; INVERTEBRATE; PROTOZOAN;

ARTICLE; CELL VIABILITY; COOLING; CRYOPRESERVATION; DIPLOPLASTRON AFFINE; EQUILIBRIUM CONSTANT; MEMBRANE TRANSPORT; NONHUMAN; PROTOZOON; THAWING;

CILIOPHORA; METADINIUM; PROTOZOA;

EID: 33646862778     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2006.01894.x     Document Type: Article

References (18)

1. Analytical Software 2003 Statistix 8 for Windows. Tallahasee, FL : Analytical Software.
2. Dehority, B.A. 1984 Evaluation of subsampling and fixation rumen procedures used for counting rumen protozoa. Appl Environ Microbiol 48, 182 185.
3. Dehority, B.A. 1993 Laboratory manual for classification and morphology of rumen ciliate protozoa. Boca Ratón, FL, USA : CRC Press.
4. Dehority, B.A. 1998 Generation times of Epidinium caudatum and Entodinium caudatum determined in vitro by transferring at various time intervals. J Anim Sci 76, 1189 1196.
5. Dumont, F., Marechal, P.A. Gervais, P. 2004 Cell size and water permeability as determinant factors for cell viability after freezing at different cooling rates. Appl Environ Microbiol 70, 268 272.
6. Fiser, P.S., Ainsworth, L. Fairful, R.V. 1987 Evaluation of a new diluent and different processing procedures for cryopreservation of ram semen. Theriogenology 28, 599 607.
7. Fondevila, M. Dehority, B.A. 2001 In vitro growth and starch digestion by Entodinium exiguum as influenced by the presence or absence of living bacteria. J Anim Sci 79, 2465 2471.
8. de la Fuente, G., Cebrián, J.A. Fondevila, M. 2004 A cryopreservation procedure for the rumen protozoon Entodinium caudatum: estimation of its viability by fluorescence microscopy. Lett Appl Microbiol 38, 164 168.
9. Harrison, R.A.P. Vickers, S.E. 1990 Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil 88, 343 352.
10. Hubálek, Z. 2003 Protectants used in the cryopreservation of microorganisms. Cryobiology 46, 205 229.
11. Kisidayova, S. 1996 The cryopreservation of some large ciliate entodiniomorphid protozoa taken from the rumen. Lett Appl Microbiol 23, 389 392.
12. Kisidayova, S. 1997 Parameters associated with optimum two-step freezing of rumen ciliate Entodinium caudatum. J Microbiol Methods 30, 119 124.
13. Kisidayova, S., Varadyova, Z., Michalowski, T. Newbold, C.J. 2005 Regeneration of cryoresistence of in vitro rumen ciliate cultures. Cryobiology 51, 76 84.
14. Marcin, A., Kisidayova, S., Fejes, J., Zelenak, I. Kmet, V. 1992 A simple technique for cryopreservation of the rumen protozoon Entodinium caudatum. Cryo-letters 13, 175 182.
15. Nsabimana, E., Kisidayova, S., Macheboeuf, D., Newbold, C.J. Jouany, J.P. 2003 Two-step freezing procedure for cryopreservation of rumen ciliates, an effective tool for creation of a frozen rumen protozoa bank. Appl Environ Microbiol 69, 3826 3832.
16. Ollero, M., Muiño Blanco, T., López Pérez, M.J. Cebrián Pérez, J.A. 1996 Surface changes associated with ram sperm cryopreservation revealed by counter-current distribution in an aqueous two-phase system: effect of different cryoprotectants. J Chromatogr B 680, 157 164.
17. Parks, J.E. Lynch, D.V. 1992 Lipid composition and thermotropic phase behaviour of boar, bull, stallion and rooster sperm membranes. Cryobiology 29, 255 266.
18. Watson, P.F. 1995 Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev 7, 871 891.