A universal cloning vector using vaccinia topoisomerase I

Molecular Biotechnology

Volumn 33, Issue 1, 2006, Pages 23-28

  Geng, Liang ^a,b   Xin, Wen ^a   Huang, Da Wei ^a,c   Feng, Gui ^a  

^a INSTITUTE OF ZOOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Universal cloning; Vaccinia topoisomerase I

Indexed keywords

POLYMERASE CHAIN REACTION; UNIVERSAL CLONING; VACCINIA TOPOISOMERASE I;

CLONING; DNA; GENETIC ENGINEERING; MOLECULAR BIOLOGY;

ENZYMES;

DNA; DNA TOPOISOMERASE; RESTRICTION ENDONUCLEASE;

ARTICLE; CLONING VECTOR; POLYMERASE CHAIN REACTION; VACCINIA VIRUS;

BASE SEQUENCE; CLONING, MOLECULAR; DNA TOPOISOMERASES, TYPE I; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; VACCINIA VIRUS;

VACCINIA VIRUS;

EID: 33646861637     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. Shuman, S. (1994) Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. J. Biol. Chem. 269, 32,678-32,684.
2. Mead, D. A., Pey, N. K., Herrnstadt, C., Marcil, R. A., Smith, L. M. (1991) A universal method for the direct cloning of PCR amplified nucleic acid. BioTechnology. 9, 657-663.
3. Magnuson, V. L., Ally, D. S., Nylund, S. J., et al. (1996) Substrate nucleotide-determined non-templated addition of adenine by Taq DNA polymerase: implications for PCR-based genotyping and cloning. Biotechniques 21, 700-709.
4. Cheng, C. H. and Shuman, S. (2000) DNA strand transfer catalyzed by vaccinia topoisomerase ligation DNAs containing a 3′ mononucleotide overhang. Nucleic Acids Res. 28, 1893-1898.