High-level expression of proteins in Escherichia coli using a pETCX10 expression system

Analytical Biochemistry

Volumn 353, Issue 2, 2006, Pages 290-292

  Shoju, Hiroyuki ^a   Sueyoshi, Noriyuki ^a   Kameshita, Isamu ^a  

^a KAGAWA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

EXPRESSION SYSTEM; HIGH LEVEL EXPRESSION;

ESCHERICHIA COLI;

CALCINEURIN; CALCIUM ION; CASEIN; ESCHERICHIA COLI PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MYELIN BASIC PROTEIN; RECOMBINANT PROTEIN; XENOPUS PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; EXPRESSION VECTOR; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE;

ESCHERICHIA COLI;

EID: 33646827333     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.02.024     Document Type: Article

References (6)

1. 2+/calmodulin-dependent protein kinase I homologue in Xenopus laevis. J. Biochem. 135 (2004) 619-630
2. 2+/calmodulin-dependent protein kinase II in Escherichia coli. J. Biochem. 138 (2005) 605-611
3. Imai Y., Matsushima Y., Sugimura T., and Terada M. A simple and rapid method for generating a deletion by PCR. Nucleic Acids Res. 19 (1991) 2785
4. Miyano O., Kameshita I., and Fujisawa H. Purification and characterization of a brain-specific multifunctional calmodulin-dependent protein kinase from rat cerebellum. J. Biol. Chem. 269 (1992) 1198-1203
5. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
6. 2+/calmodulin-dependent protein kinase IV kinase isoforms in rat brain. J. Biochem. 119 (1996) 1176-1181