Late gene mutants of bacteriophage λ as an efficient expression vector

Enzyme and Microbial Technology

Volumn 39, Issue 3, 2006, Pages 420-425

  Oh, Jeong Seok ^a   Park, Tai Hyun ^a  

^a Seoul National University   (South Korea)

Author keywords

Bacteriophage late gene mutants; Escherichia coli; Plasmid instability; Stable expression system; Translational efficiency

Indexed keywords

CLONING; DNA; ESCHERICHIA COLI; GENES; MUTAGENESIS;

BACTERIOPHAGE Λ LATE GENE MUTANTS; PLASMID INSTABILITY; STABLE EXPRESSION SYSTEM; TRANSLATIONAL EFFICIENCY;

BACTERIOPHAGES;

BACTERIAL DNA; BETA GALACTOSIDASE; MESSENGER RNA;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIOPHAGE; CELL GROWTH; CONTROLLED STUDY; CYTOLYSIS; DNA PACKAGING; DNA REPLICATION; EXPRESSION VECTOR; GENE MUTATION; GENE REPRESSION; GENETIC STABILITY; GENETIC TRANSCRIPTION; HOST CELL; NONHUMAN; RNA TRANSLATION;

ENTEROBACTERIA PHAGE LAMBDA;

EID: 33646772181     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.11.037     Document Type: Article

References (17)

1. Johnvesly B., Kang D.G., Choi S.S., Kim J.H., and Cha H.J. Comparative production of green fluorescent protein under co-expression of bacterial hemoglobin in Escherichia coli W3110 using different culture scale. Biotechnol Bioprocess Eng 9 (2004) 274-277
2. Kim O.S., Kwak H.J., Lee J.-H., Ha J.M., Ha B.J., and Lee S.-H. Development of an E. coli expression cassette for the efficient production of a harmful protein. Biotechnol Bioprocess Eng 9 (2004) 389-392
3. Choi J.-I., and Lee S.Y. High level production of supra molecular weight poly (3-hydroxybutyrate) by metabolically engineered Escherichia coli. Biotechnol Bioprocess Eng 9 (2004) 196-200
4. Hong S.H., and Lee S.Y. Enhanced production of succinic acid by metabolically engineered Escherichia coli with amplified activities of malic enzyme and fumarase. Biotechnol Bioprocess Eng 9 (2004) 252-255
5. Park T.H., Seo J.H., and Lim H.C. Two-stage fermentation with bacteriophage λ as an expression vector in Escherichia coli. Biotechnol Bioeng 37 (1991) 297-302
6. - mutant for stable and efficient production of recombinant protein in Escherichia coli system. Biotechnol Bioeng 57 (1998) 529-535
7. Padukone N., Peretti S.W., and Ollis D.F. Characterization of the mutant lytic state in lambda expression systems. Biotechnol Bioeng 36 (1992) 369-377
8. Elizabeth J.G., and Roberts J.W. The phage λQ gene product: activity of a transcription antiterminator in vitro. Cell 30 (1982) 637-648
9. Carol A.M., and Murray N.E. T4 polynucleotide kinase; Cloning of the gene (pseT) and amplification of its product. EMBO J 4 (1985) 2695-2703
10. --contain recombinant Escherichia coli fermentation process. Bioprocess Eng 23 (2000) 187-190
11. Simons R.W., Houman F., and Kleckner N. Improved signal and muticopy lac-based cloning vectors for protein and operon fusions. Gene 53 (1987) 85-96
12. Hill C., Massey I.T., and Klanehammer T.R. Rapid method to characterize lactococcal bacteriophage genomes. Appl Environ Microbiol 57 (1991) 283-288
13. Michael W.P., and Hageleit M. Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR. Biotechnol Lett 23 (2001) 275-282
14. Chen B.Y., Lin C.S., and Lim H.C. Temperature induction of bacteriophage λ mutants in Escherichia coli. J Biotechnol 40 (1995) 87-97
15. Karol T., and Wegrzyn G. Replication of coliphage lambda DNA. FEMS Microbiol Rev 17 (1995) 109-119
16. Sambrook J., and Russell D.W. Molecular cloning. A laboratory manual. 3rd ed. (2001), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY p. 2.1-2.24
17. Jesper V., Michael A.S., Michael D.R., and Pederson S. Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes: expression from reporter genes does not always reflect functional mRNA levels. J Mol Biol 231 (1993) 678-688