Expression, purification, and immobilization of His-tagged D-amino acid oxidase of Trigonopsis variabilis in Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 70, Issue 6, 2006, Pages 683-689

  Zheng, Huabao ^a,b   Wang, Xiaolan ^b,c   Chen, Jun ^b   Zhu, Ke ^d   Zhao, Yuhua ^a   Yang, Yunliu ^b   Yang, Sheng ^b   Jiang, Weihong ^b  

^a ZHEJIANG UNIVERSITY   (China)

^b INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^c JIANGXI NORMAL UNIVERSITY   (China)

^d Shijiazhuang Pharmaceutical Group   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; GENES; METHANOL; MOLECULAR WEIGHT; PURIFICATION; YEAST;

AFFINITY PURIFICATION; AGAR-BASED AFFINITY; INTRACELLULAR PRODUCTION; METHANOL FEEDING;

ENZYME IMMOBILIZATION;

AGAR; ALCOHOL OXIDASE; DEXTRO AMINO ACID OXIDASE; ETHYLENE OXIDE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HISTIDINE; MATING HORMONE ALPHA FACTOR; METHANOL; RESIN;

ALCOHOL; AMINO ACID; ENZYME ACTIVITY; IMMOBILIZATION; YEAST;

ARTICLE; CONCENTRATION (PARAMETERS); DAO GENE; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME SYNTHESIS; FUNGAL GENE; GENE EXPRESSION REGULATION; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; TRIGONOPSIS VARIABILIS; YEAST;

BIOREACTORS; D-AMINO-ACID OXIDASE; ENZYMES, IMMOBILIZED; GENE EXPRESSION REGULATION, FUNGAL; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HISTIDINE; INDUSTRIAL MICROBIOLOGY; PICHIA; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; SACCHAROMYCETALES;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; TRIGONOPSIS VARIABILIS;

EID: 33646762489     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0158-8     Document Type: Article

References (20)

1. Alonso J, Barredo JL, Armisen P, Diez B, Salto F, Guisan JM, Garcia JL, Cortes E (1999) Engineering the D-amino acid oxidase from Trigonopsis variabilis to facilitate its overproduction in Escherichia coli and its downstream processing by tailor-made metal chelate supports. Enzyme Microb Technol 25:88-95
2. Bai J, Rodriguez AM, Melendez JA, Cederbaum AI (1999) Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. J Biol Chem 274:26217-26224
3. Barber MS, Giesecke U, Reichert A, Minas W (2004) Industrial enzymatic production of cephalosporin-based beta-lactams. In: Molecular biotechnology of fungal beta-lactam antibiotics and related peptide synthetases. Springer, Berlin Heidelberg New York, pp 179-215
4. Becka S, Skrob F, Plhackova K, Kujan P, Holler P, Kyslik P (2003) Cross-linked cell aggregates of Trigonopsis variabilis: D-amino acid oxidase catalyst for oxidation of cephalosporin C. Biotechnol Lett 25:227-233
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
6. Cambiaghi S, Tomaselli S, Verga R (1991) Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives. EP0496993A1
7. Furuya K, Matsuda A (1993) A transformant capable of producing D-amino acid oxidase. EP583817A2
8. Golini P, Bianchi D, Battistel E, Cesti P, Tassinari R (1995) Immobilization of D-amino acid oxidase from different yeasts-characterization and application in the deamination of cephalosporin C. Enzyme Microb Technol 17:324-329
9. Gonzalez FJ, Montes J, Martin F, Lopez MC, Ferminan E, Catalan J, Galan MA, Dominguez A (1997) Molecular-cloning of TvDAO1, a gene encoding a D-amino acid oxidase from Trigonopsis variabilis and its expression in Saccharomyces cerevisiae and Kluyveromyces lactis. Yeast 13:1399-1408
10. Hemdan ES, Zhao YJ, Sulkowski E, Porath J (1989) Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography. Proc Natl Acad Sci U S A 86:1811-1815
11. Horner R, Wagner F, Fischer L (1996) Induction of the D-amino acid oxidase from Trigonopsis variabilis. Appl Environ Microbiol 62:2106-2110
12. Hubert P, Porath J (1980) Metal chelate affinity chromatography. I. Influence of various parameters on the retention of nucleotides and related compounds. J Chromatogr 198:247-255
13. Hwang TS, Fu HM, Lin LL, Hsu WH (2000) High-level expression of Trigonopsis variabilisD-amino acid oxidase in Escherichia coli using lactose as inducer. Biotechnol Lett 22:655-658
14. Isoai A, Kimura H, Reichert A, Schorgendorfer K, Nikaido K, Tohda H, Giga-Hama Y, Mutoh N, Kumagai H (2002) Production of D-amino acid oxidase (DAO) of Trigonopsis variabilis in Schizosaccharomyces pombe and the characterization of biocatalysts prepared with recombinant cells. Biotechnol Bioeng 80:22-32
15. Lin LL, Chien HR, Wang WC, Hwang TS, Fu HM, Hsu WH (2000) Expression of Trigonopsis variabilisD-amino acid oxidase gene in Escherichia coli and characterization of its inactive mutants. Enzyme Microb Technol 27:482-491
16. Liu HB, Jiang WH, Yang YL (1999) Cloning, sequencing and expression of D-amino acid oxidase gene. Chin J Biotechnol 15:337-342
17. Pilone MS, Pollegioni L, Buto S (1992) Stability and kinetic-properties of immobilized Rhodotorula gracilisD amino acid oxidase. Biotechnol Appl Biochem 16:252-262
18. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
19. Sauber K (1993) Lessons from industry. In: van den Tweel JJ, Harder A, Buitelaar RM (eds) Stability and stabilization of enzymes. Elsevier, Amsterdam, pp 145-151
20. Yu J, Li DY, Zhang YJ, Yang S, Li RB, Yuan ZY (2002) High expression of Trigonopsis variabilisD-amino acid oxidase in Pichia pastoris. J Mol Catal B Enzym 18:291-297