Evaluation of simplified DNA extraction methods for emm typing of group a streptococci

Indian Journal of Medical Microbiology

Volumn 24, Issue 2, 2006, Pages 127-130

  Jose, J J M ^a   Brahmadathan, K N ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

Author keywords

Direct colony PCR; Emm typing; Group A Streptococci (GAS)

Indexed keywords

HYALURONIDASE; LYSINE DERIVATIVE; MUTANOLYSIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CELL LYSATE; COOLING; DNA EXTRACTION; DNA SEQUENCE; EMM GENE; FREEZING; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; STREPTOCOCCUS GROUP A;

EID: 33646741217     PISSN: 02550857     EISSN: 02550857     Source Type: Journal    
DOI: 10.4103/0255-0857.25200     Document Type: Article

References (13)

1. Cunningham MW. Pathogenesis of Group A Streptococcal infections. Clin Microbiol Rev 2000;13:470-511.
2. Padmavati S. Rheumatic fever and rheumatic heart disease in India at the turn of the century. Indian Heart J 2001;53:35-7.
3. Hu MC, Walls MA, Stroop SD, Reddish MA, Beall B, Dale JB. Immunogenicity of a 26 valent Group A Streptococcal Vaccine. Infect Immun 2002;70:2171-7.
4. Beall B, Facklam R, Thompson T. Sequencing emm-specific PCR products foe routine and accurate typing of Group A Streptococci. J Clin Microbiol 1996;34:953-8.
5. Wilson IG. Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 1997;63:3741-51.
6. Sandhu GS, Precup JW, Kline BC. Rapid one step characterization of recombinant vectors by direct analysis of transformed Escherichia coli colonies. Biotechniques 1989;7:689-90.
7. Gussow D, Clackson T. Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 1989;17:4000.
8. Hofmann MA, Brian DA. Sequencing PCR DNA amplified directly from a bacterial colony. Biotechniques 1991;11:30-1.
9. Pandian R, Brahmadathan KN, Mathai E. Throat and Nose. In: Myer's and Koshi's manual of Diagnostic Procedures in Medical Microbiology and Immunology/Serology. Faculty, Department of Clinical Microbiology, Christian Medical College, Vellore, India. All India Press: Poindicherry, India; 2001. p. 52-6.
10. Podbielski A, Melzer B, Lutticken R. Application of polymerase chain reaction to study the M protein (-like) gene family in β-hemolytic streptococci. Med Microbiol Immunol 1991;180:213-27.
11. Hynes WL, Ferretti JJ, Gilmore MS, Segarra RA. PCR amplification of Streptoccal DNA using crude cell lysates. FEMS Microbiol Lett 1992;94:139-42.
12. Yu CE, Ferretti JJ. Molecular epidemiologic analysis of the type A streptococcal exotoxin (erythrogenic toxin) gene (speA) in clinical Streptococcus pyogenes strains. Infect Immun 1989;57:3715-9.
13. Kang HP, Dunne WM. Stability of Repetitive-Sequence PCR patterns with respect to culture age and subculture frequency. J Clin Microbiol 2003;41:2694-6.