Cloning, expression, and characterization of a hyperalkaline phosphatase from the thermophilic bacterium Thermus sp. T351

Journal of Microbiology and Biotechnology

Volumn 16, Issue 2, 2006, Pages 272-279

  Choi, Jeong Jin ^a   Park, Jong Woo ^a   Shim, Hyekyung ^a   Lee, Sukchan ^a   Kwon, Moosik ^a   Yang, Joo Sung ^a   Hwang, Heon ^a   Kwon, Suk Tae ^a  

^a Sungkyunkwan University   (South Korea)

Author keywords

Alkaline phosphatase; Sequence analysis; Thermostable enzyme; Thermus sp. T351

Indexed keywords

4 NITROPHENYLPHOSPHATASE; COBALT; EDETIC ACID; MAGNESIUM ION; METAL ION; PHOSPHATASE; SIGNAL PEPTIDE;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; HALF LIFE TIME; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PHOSPHORYLATION; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE DEPENDENCE; THERMOPHILIC BACTERIUM; THERMUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMUS; THERMUS SP.;

EID: 33646692199     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Bradshaw, R. A., F. Cancedda, L. H. Ericsson, P. A. Neumann, S. P. Piccoli, M. J. Schlesinger, K. Shriefer, and K. A. Walsh. 1981. Amino acid sequence of Escherichia coli alkaline phosphatase. Proc. Natl. Acad. Sci. USA 78: 3473-3477.
2. Chang, C. N., W.-J. Kuang, and E. Y. Chen. 1986. Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli. Gene 44: 121-125.
3. Chen, P. S., T. Y. Toribara, and H. Warner. 1956. Microdetermination of phosphorus. Anal. Chem. 28: 1756-1758.
4. Choi, J. J. and S.-T. Kwon. 2004. Cloning, expression, and characterization of DNA polymerase from hyperthermophilic bacterium Aquifex pyrophilus. J. Microbiol. Biotechnol. 14: 1022-1030.
5. Coleman, J. E. 1992. Structure and mechanism of alkaline phosphatase. Annu. Rev. Biophys. Biomol. Struct. 21: 441-483.
6. Coleman, J. E. and P. Gettins. 1983. Molecular properties and mechanism of alkaline phosphatase, pp. 153-217. In T. Spiro (ed.), Metal Ion in Biology, vol. 5. John Wiley and Sons, New York, U.S.A.
7. Corpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16: 10881-10890.
8. Dong, G. and J. G. Zeikus. 1997. Purification and characterization of alkaline phosphatase from Thermotoga neapolitana. Enzyme Microb. Technol. 21: 335-340.
9. Gong, N., C. Chen, L. Xie, H. Chen, X. Lin, and R. Zhang. 2005. Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp. nov. and investigation of its cobalt activation at high temperature. Biochim. Biophys. Acta 1750: 103-111.
10. Hanahan, D. and M. Meselson. 1980. Plasmid screening at high colony density. Gene 10: 63-67.
11. Jung, S. E., J. J. Choi, H. K. Kim, and S.-T. Kwon. 1997. Cloning and analysis of the DNA polymerase-encoding gene from Thermus filiformis. Mol. Cells 7: 769-776.
12. Kang, S. K., K. K. Cho, J. K. Ahn, S. H. Kang, K. H. Han, H. G. Lee, and Y. J. Choi. 2004. Cloning and expression of thermostable β-glycosidase gene from Thermus filiformis Wai33 A1 in Escherichia coli and enzyme characterization. J. Microbiol. Biotechnol. 14: 584-592.
13. Kim, E. E. and H. W. Wyckoff. 1991. Reaction mechanism of alkaline phosphatase based on crystal structures. J. Mol. Biol. 218: 449-464.
14. Kim, Y.-J., T.-S. Park, H.-H. Kim, and S.-T. Kwon. 1997. Purification and characterization of a thermostable alkaline phosphatase produced by Thermus caldophilus GK24. J. Biochem. Mol. Biol. 30: 262-268.
15. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
16. Lee, J.-H., Y.-D. Cho, J. J. Choi, Y.-J. Lee, H.-S. Hoe, H.-K. Kim, and S.-T. Kwon. 2003. High-level expression in Escherichia coli of alkaline phosphatase from Thermus caldophilus GK24 and purification of the recombinant enzyme. J. Microbiol. Biotechnol. 13: 660-665.
17. Lowry, O. H., N. J. Rosebrough, A. J. Farr, and R. J. Randall. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275.
18. Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3: 208-218.
19. Maunders, M. J. 1993. Alkaline phosphatase, pp. 331-341. In M. M. Burrell (ed.), Enzymes of Molecular Biology. Humana Press, New Jersey, U.S.A.
20. McComb, R. B., G. N. Bowers, and S. Posen. 1979. Alkaline Phosphatase. Plenum Press, New York, U.S.A.
21. Mori, S., M. Okamoto, M. Nishibori, M. Ichimura, J. Sakiyama, and H. Endo. 1999. Purification and characterization of alkaline phosphatase from Bacillus stearothermophilus. Biotechnol. Appl. Biochem. 29: 235-239.
22. Murphy, J. E., T. T. Tibbitts, and E. R. Kantrowitz. 1995. Mutations at positions 153 and 328 in Escherichia coli alkaline phosphatase provide insight towards the structure and function of mammalian and yeast alkaline phosphatases. J. Mol. Biol. 253: 604-617.
23. Murphy, J. E., X. Xu, and E. R. Kantrowitz. 1993. Conversion of a magnesium binding site into a zinc binding site by a single amino acid substitution in Escherichia coli alkaline phosphatase. J. Biol. Chem. 268: 21497-21500.
24. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10: 1-6.
25. Onishi, H. R., J. S. Tkacz, and J. O. Lampen. 1979. Glycoprotein nature of yeast alkaline phosphatase: Formation of active enzyme in the presence of tunicamycin. J. Biol. Chem. 254: 11943-11952.
26. Park, T., J.-H. Lee, H.-K. Kim, H.-S. Hoe, and S.-T. Kwon. 1999. Nucleotide sequence of the gene for alkaline phosphatase of Thermus caldophilus GK24 and characteristics of the deduced primary structure of the enzyme. FEMS Microbiol. Lett. 180: 133-139.
27. Plebani, M., D. Bernardi, M. Zaninotto, M. De Paoli, S. Secchiero, and L. Sciacovelli. 1996. New and traditional serum markers of bone metabolism in the detection of skeletal metastases. Clin. Biochem. 29: 67-72.
28. Reid, T. W. and I. B. Wilson. 1971. E. coli phosphatase, pp. 373-415. In P. D. Boyer (ed.), Enzymes, vol. 4. Academic Press, London, U.K.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York, U.S.A.
30. Shin, H.-J., S.-K. Lee, J. J. Choi, S. Koh, J.-H. Lee, S.-J. Kim, and S.-T. Kwon. 2005. Cloning, expression, and characterization of a family B-type DNA polymerase from the hyperthermophilic crenarchaeon Pyrobaculum arsenaticum and its application to PCR. J. Microbiol. Biotechnol. 15: 1359-1367.
31. Shine, J. and L. Dalgarno. 1975. Determination of cistron specificity in bacterial ribosomes. Nature 254: 34-38.
32. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with Gene Fusions. Cold Spring Harbor Laboratory Press, New York, U.S.A.
33. Stec, B., K. M. Holtz, and E. R. Kantrowitz. 2000. A revised mechanism for the alkaline phosphatase reaction involving three metal ions. J. Mol. Biol. 299: 1303-1311.
34. Studier, F. W. and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130.
35. L-alkaline phosphatase fusion proteins. J. Immunol. Methods 224: 171-184.
36. Wojciechowski, C. L., J. P. Cardia, and E. R. Kantrowitz. 2002. Alkaline phosphatase from the hyperthermophilic bacterium T. maritima requires cobalt for activity. Protein Sci. 11: 903-911.
37. Yeh, M.-F. and J. M. Trela. 1976. Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus. J. Biol. Chem. 251: 3134-3139.
38. Zappa, S., J.-L. Rolland, D. Flament, Y. Gueguen, J. Boudrant, and J. Dietrich. 2001. Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi. Appl. Environ. Microbiol. 67: 4504-4511.