A mismatch-selective bifunctional rhodium-Oregon Green conjugate: A fluorescent probe for mismatched DNA

Journal of the American Chemical Society

Volumn 128, Issue 17, 2006, Pages 5654-5655

  Zeglis, Brian M ^a   Barton, Jacqueline K ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; METAL COMPLEX; PHOSPHORUS 32; RHODIUM COMPLEX;

ARTICLE; AUTORADIOGRAPHY; BASE MISPAIRING; BASE PAIRING; COMPLEX FORMATION; DNA REPLICATION; DNA SEQUENCE; FLUORESCENCE; ISOTOPE LABELING; SYNTHESIS;

AUTORADIOGRAPHY; BASE PAIR MISMATCH; CARBOXYLIC ACIDS; COLORING AGENTS; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; RHODIUM;

EID: 33646592546     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja061409c     Document Type: Article

References (22)

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16. Several linker-modified bipyridine linkers have been examined. Alkane linkers in particular lead to significant quenching under all conditions.
17. For Oregon Green excitation and emission maxima are 514 and 525 nm.
18. A 475-mn excitation wavelength was employed in all experiments.
19. For comparison, without DNA, the relative fluorescent intensity for 1 μM 1: Oregon Green + 2: Oregon Green are 1/73/100.
20. These titrations do not allow quantitation of affinities. Qualitatively, results for 1 with mismatched DNA agree with those for the parent complex. See refs 3 and 4 as well as photocleavage results in Figure 3.
21. Sitlani, A.; Long, E. C.; Pyle, A. M.; Barton, J. K. J. Am. Chem. Soc. 1992, 114, 2303-2312.
22. This value is ∼10% lower, which may reflect the decreased charge for 1.