Expression optimization and purification process development of an engineered soluble recombinant mouse linker of activation of T cells using surface enhanced laser desorption/ionization-mass spectrometry

Protein Expression and Purification

Volumn 47, Issue 2, 2006, Pages 533-541

  Brenac, Virginie ^a   Mouz, Nicolas ^b   Schapman, Anthony ^a   Ravault, Vincent ^a  

^a Pall BioSepra ^*  (France)

^b Protein'eXpert   (France)

Author keywords

His tagged recombinant protein; Immobilized metal affinity chromatography; Protein microarray; Soluble linker of activation of T cells; Surface enhanced laser desorption ionization mass spectrometry

Indexed keywords

LAT PROTEIN, MOUSE; MEMBRANE PROTEIN; PHOSPHOPROTEIN; RECOMBINANT PROTEIN; SIGNAL TRANSDUCING ADAPTOR PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOUSE; PEPTIDE MAPPING; PROTEIN ENGINEERING; PROTEIN MICROARRAY;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; MEMBRANE PROTEINS; MICE; PEPTIDE MAPPING; PHOSPHOPROTEINS; PROTEIN ARRAY ANALYSIS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 33646553081     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.02.021     Document Type: Article

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