A cotton-specific gene, stearoyl-ACP desaturase, used as a reference for qualitative and real-time quantitative polymerase chain reaction detection of genetically modified organisms

Journal of the Science of Food and Agriculture

Volumn 86, Issue 7, 2006, Pages 1103-1109

  Xu, Wen Tao ^a   Huang, Kun Lun ^a   Wang, Ying ^a   Zhang, Hong Xing ^a   Luo, Yun Bo ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cotton; Genetically modified organisms; Polymerase chain reaction; Sad1 gene; Stearoyl ACP desaturase

Indexed keywords

CAPSICUM ANNUUM VAR. ANNUUM; CUCUMIS SATIVUS; DAUCUS CAROTA; GLYCINE MAX; GOSSYPIUM HIRSUTUM; HELIANTHUS; HORDEUM VULGARE SUBSP. VULGARE; LYCOPERSICON ESCULENTUM; MALUS X DOMESTICA; NICOTIANA TABACUM; PISUM SATIVUM; PRUNUS PERSICA; SESAMUM INDICUM; SINAPIS ARVENSIS; SOLANUM TUBEROSUM; ZEA MAYS;

EID: 33646546372     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.2464     Document Type: Article

References (14)

1. Luo YB and Huang KL, Blue Papers of Science and Technology of the China Food Industry. The Chinese Institute of Food Science and Technology, Beijing, China, 115-119 (2005).
2. Meyer R, Development and application of DNA analytical methods for the detection of GMOs in food. Food Control 10:391-399 (1999).
3. Brodmann PD, Ilg EC, Berthoud H and Herrmann A, Real-time quantitative polymerase chain reaction methods for four genetically modified maize varieties and maize DNA. J AOAC Int 85:646-653 (2002).
4. Petit L, Baraige F, Balois AB, Bertheau Y and Fach P, Screening of genetically modified organisms and specific detection of Bt176 maize in flours and starches by PCR-enzyme linked immunosorbent assay. Eur Food Res Technol 217:83-89 (2003).
5. Meyer R, Chardonnens F, Hubner P and Luthy J, Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products. Z Lebensm Unters Forsch 203:339-344 (1996).
6. Delano J, Schmidt AM, Wall E, Green M and Masri S, Reliable detection and identification of the genetically modified maize, soybean and canola by multiplex PCR analysis. J Agric Food Chem 51:5839-5834 (2003).
7. Holst-Jensen A, GMO detection method using PCR review. Available: www.entransfood.com, 10 (2003).
8. Zimmernann A, Hemmer W, Liniger M, Lüthy J and Pauli U, A sensitive detection method for genetically modified MaisGard corn using a nested PCR system. Lebensm-Wiss Technol 31:664-667 (1998).
9. Vollenhofer S, Burg K, Schmidt J and Kroath H, Genetically modified organism in food-screening and specific detection by polymerase chain reaction. J Agric Food Chem 47:5038-5043 (1999).
10. Hernandez M, Ryo A, Esteve T, Prat S and Pla M, A rapeseed specific gene, acetyl-CoA carboxylase, can be used as a reference for qualitative and quantitative PCR detection of transgenes from the mixed samples. J Agric Food Chem 49:3622-3627 (2001).
11. Ding JY, Jia JW, Yang LT, Zhang CM and Zhang DB, Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem 52:3372-3377 (2004).
12. Sambrook J and Russell DW, Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2001).
13. Zhou MY, Xue D, Gomez-Sanchez EP and Gomez-Sanchez CE, Improved downward capillary transfer for blotting of DNA and RNA. Biotechniques 16:58-59 (1994).
14. Arumugannathan K and Earle ED, Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208-218 (1991).