Nuclease P1 digestion/high-performance liquid chromatography, a practical method for DNA quantitation

Journal of Chromatography A

Volumn 1117, Issue 2, 2006, Pages 132-136

  Shimelis, Olga ^a   Giese, Roger W ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

Author keywords

Acid hydrolysis of DNA; DNA digestion; DNA purification; DNA quantification; HPLC; Impure DNA; Nuclease P1

Indexed keywords

DNA; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ION EXCHANGE; NEGATIVE IONS; PHOSPHATES;

ACID HYDROLYSIS OF DNA; DNA DIGESTION; DNA PURIFICATION; DNA QUANTIFICATION; HPLC; IMPURE DNA; NUCLEASE P1;

ENZYME KINETICS;

DYE; GENOMIC DNA; NUCLEASE; NUCLEASE P1; UNCLASSIFIED DRUG;

ACCURACY; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CATTLE; DNA DETERMINATION; DNA EXTRACTION; DNA PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; INTERMETHOD COMPARISON; LIGHT ABSORPTION; LIVER; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SWINE; THYMUS;

ANIMALS; ASPERGILLUS NUCLEASE S1; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; SPECTROPHOTOMETRY, ULTRAVIOLET;

BOS TAURUS; SUS SCROFA;

EID: 33646527965     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2006.03.071     Document Type: Article

References (19)

1. Glasel G.A. Biotechniques 18 (1995) 62
2. Morinello E.J., Ham A.-J.L., Ranasinghe A., Nakamura J., Upton P.B., and Swenberg J.A. Cancer Res. 62 (2002) 5189
3. Ham A.-J., Ranasinghe A., Morinello E.J., Nakamura J., Upton P.B., Johnson F., and Swenberg J.A. Chem. Res. Toxicol. 12 (1999) 1240
4. Boucheron J.A., Richardson F.C., Morgan P.H., and Swenberg J.A. Cancer Res. 47 (1987) 1577
5. Huang X., Powell J., Mooney L.A., Li C., and Frenkel K. Free Radical Biol. Med. 31 (2001) 1341
6. Barbin A., Ohgaki H., Nakamura J., Kurrer M., Kleihues P., and Swenberg J.A. Cancer Epidemiol. Biomarkers Prev. 12 (2003) 1241
7. Li G., Shimelis O., Zhou X., and Giese R.W. Biotechniques 34 (2003) 908
8. Shimelis O., Zhou X., Li G., and Giese R.W. J. Chromatogr. A 1004 (2004) 47
9. Qiagen Genomic DNA Handbook, Qiagen, Valencia, CA, 2001.
10. DyNA Quant 200 Capillary Cuvette Kit Instructions, Hoefer Pharmacia Biotech, San Francisco, CA, 1995.
11. In: Fasman G. (Ed). CRC Handbook of Biochemistry and Molecular Biology, Nucleic Acids. 3rd ed. 1 (1975), CRC Press, Boca Raton, FL
12. Fisher D.H., and Giese R.W. J. Chromatogr. 452 (1988) 51
13. Ravanat J.-L., Gremaud E., Markovic J., and Turesky R.J. Anal. Biochem. 260 (1988) 30
14. Valencia R., Cong H.N., and Bertaux O. J. Chromatogr. 325 (1985) 207
15. Eick D., Fritz H.J., and Doerfler W. Anal. Biochem. 135 (1983) 165
16. Swarts S.G., Smith G.S., and Wheeler K.T. Radiat. Environ. Biophys. 35 (1996) 41
17. Ko C.Y., Johnson J.L., Barnett L.B., McNair H.M., and Vercellotti J.R. Anal. Biochem. 80 (1977) 183
18. Zamenhof S. Meth. Enzymol. 3 (1957) 696
19. Frelon S., Douki T., Ravanat J.-L., Pouget J.-P., Tornabene C., and Cadet J. Chem. Res. Toxicol. 13 (2000) 1002