In vitro refolded napin-like protein of Momordica charantia expressed in Escherichia coli displays properties of native napin

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1764, Issue 5, 2006, Pages 847-855

  Vashishta, Aruna ^a   Sahu, Tejram ^a   Sharma, Anshu ^a   Choudhary, Shailesh Kumar ^a   Dixit, Aparna ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

antifungal activity; in vitro refolding; Momordica charantia; Napin like protein cDNA; recombinant napin

Indexed keywords

ALBUMIN; AMINO ACID; AMPHOTERICIN B; BUFFER; COMPLEMENTARY DNA; DISULFIDE; GLUTATHIONE; GLUTATHIONE DISULFIDE; GLYCEROL; HETERODIMER; HISTONE; NAPIN LIKE PROTEIN; NICKEL; NITRILOTRIACETIC ACID; PEPTIDE; PLASMID VECTOR; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ALPHA HELIX; AMINO TERMINAL SEQUENCE; ANTIFUNGAL ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; CELL INCLUSION; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOSOL; DENATURATION; DILUTION; DRUG ISOLATION; DRUG PURIFICATION; ENVIRONMENT; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE EXPRESSION; GENE LIBRARY; GENETIC CODE; HETEROLOGOUS EXPRESSION; IC 50; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MOLECULAR CLONING; MOMORDICA CHARANTIA; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OXIDATION REDUCTION REACTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; REDUCTION KINETICS; SIGNAL TRANSDUCTION; SOLUBILIZATION; SPECTROSCOPY; TRICHODERMA;

AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MOMORDICA CHARANTIA; PLANT PROTEINS; PROTEIN FOLDING; RECOMBINANT PROTEINS; TRICHODERMA;

ESCHERICHIA COLI; MOMORDICA CHARANTIA; TRICHODERMA; TRICHODERMA HAMATUM;

EID: 33646521715     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2006.03.010     Document Type: Article

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