Detection of proteins by on-column, non-covalent labeling with NanoOrange during capillary zone electrophoresis

Journal of Liquid Chromatography and Related Technologies

Volumn 29, Issue 10, 2006, Pages 1447-1455

  Feng, Hua Tao ^a   Li, Sam Fong Yau ^a   Chia, Pei Shan ^b   Loke, Weng Keong ^b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b DSO NATIONAL LABORATORIES   (Singapore)

Author keywords

Capillary electrophoresis; Laser induced fluorescence; NanoOrange; Non covalent labeling; Proteins

Indexed keywords

CAPILLARITY; CAPILLARY FLOW; ELECTROPHORESIS; FLUORESCENCE; PH EFFECTS; SPECTROPHOTOMETERS;

CAPILLARY ELECTROPHORESIS; LASER INDUCED FLUORESCENCE; NANOORANGE; NONCOVALENT LABELING;

PROTEINS;

BUFFER; DODECYL SULFATE SODIUM; DYE; NANOORANGE; PROTEIN; REAGENT; STAPHYLOCOCCUS ENTEROTOXIN B; UNCLASSIFIED DRUG;

ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; DIAGNOSTIC VALUE; FLUORESCENCE; LASER INDUCED FLUORESCENCE; PH; PROTEIN DETERMINATION; SPECTROFLUOROMETRY;

STAPHYLOCOCCUS;

EID: 33646467805     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070600674844     Document Type: Article

References (23)

1. Cooper, J.W.; Wang, Y.J.; Lee, C.S. Recent advances in capillary separations for proteomics. Electrophoresis 2004, 25 (23-24), 3913-3926.
2. Bandilla, D.; Skinner, C.D. Capillary electrochromatography of peptides and proteins. J. Chromatogr. A 2004, 1044 (1-2), 113-129.
3. Hutterer, K.; Dolnik, V. Capillary electrophoresis of proteins 2001-2003. Electrophoresis 2003, 24 (22-23), 3998-4012.
4. Swaile, D.; Sepaniak, M. Laser-based fluorometric detection schemes for the analysis of proteins by CZE. J. Liq. Chromatogr. 1991, 14 (5), 869-893.
5. Colyer, C. Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection. Cell Biochem. Biophys. 2000, 33 (3), 323-337.
6. Welder, F.; Paul, B.; Nakazumi, H.; Yagi, S.; Colyer, C.L. Symmetric and asymmetric squarylium dyes as noncovalent protein labels: a study by fluorimetry and capillary electrophoresis. J. Chromatogr. B 2003, 793 (1), 93-105.
7. McCorquodale, E.M.; Colyer, C.L. Indocyanine green as a noncovalent, pseudofluorogenic label for protein determination by capillary electrophoresis. Electrophoresis 2001, 22 (12), 2403-2408.
8. Patonay, G.; Salon, J.; Sowell, J.; Strekowski, L. Non-covalent labeling of biomolecules with red and near-infrared dyes. Molecules 2004, 9 (3), 40-49.
9. Benito, I.; Marina, M.L.; Saz, J.M.; Diez-Masa, J.C. Detection of bovine whey proteins by on-column derivatization capillary electrophoresis with laser-induced fluorescence monitoring. J. Chromatogr. A 1999, 841 (1), 105-114.
10. Liu, Y.J.; Foote, R.S.; Jacobson, S.C.; Ramsey, R.S.; Ramsey, J.M. Electrophoretic separation of proteins on a microchip with noncovalent, postcolumn labeling. Anal. Chem. 2000, 72 (19), 4608-4613.
11. Jin, L.J.; Giordano, B.C.; Landers, J.P. Dynamic labeling during capillary or microchip electrophoresis for laser-induced fluorescence detection of protein-SDS complexes without pre- or postcolumn labeling. Anal. Chem. 2001, 73 (20), 4994-4999.
12. Sano, M.; Nishino, I.; Ueno, K.; Kamimori, H. Assay of collagenase activity for native triple-helical collagen using capillary gel electrophoresis with laser-induced fluorescence detection. J. Chromatogr. B 2004, 809 (2), 251-256.
13. Sze, N.S.K.; Huang, T.M.; Pawliszyn, J. Laser-induced fluorescence detection of noncovalently labeled protein in capillary isoelectric focusing. J. Sep. Sci. 2000, 25 (15-17), 1119-1122.
14. Harvey, M.D.; Bablekis, V.; Banks, P.R.; Skinner, C.D. Utilization of the non-covalent fluorescent dye, NanoOrange, as a potential clinical diagnostic tool-Nanomolar human serum albumin quantitation. J. Chromatogr. B 2001, 754 (2), 345-356.
15. Jones, L.J.; Haugland, R.P.; Singer, V.L. Development and characterization of the NanoOrange (R) protein quantitation assay: A fluorescence-based assay of proteins in solution. Biotechniques 2003, 34 (4), 850.
16. Grossart, H.P.; Steward, G.F.; Martinez, J.; Azam, F. A simple, rapid method for demonstrating bacterial flagella. Appl. Environ. Microbiol. 2000, 66 (8), 3632-3636.
17. Kuroda, Y.; Maeda, Y.; Sawa, S.; Shibata, K.; Miyamoto, K.; Nakagawa, T. Effects of detergents on the secondary structures of prion protein peptides as studied by CD spectroscopy. J. Pep. Sci. 2003, 9 (4), 212-220.
18. Stutz, H.; Wallner, H.M., Jr.; Bordin, G.; Rodriguez, A.R. Detection of coexisting protein conformations in capillary zone electrophoresis subsequent to transient contact with sodium dodecyl sulfate solutions. Electrophoresis 2005, 26 (6), 1089-1105.
19. Rusnak, J.M.; Kortepeter, M.; Ulrich, R.; Poli, M.; Boudreau, E. Laboratory exposures to staphylococcal enterotoxin B. Emerg. Infec. Dis. 2004, 10 (9), 1544-1549.
20. Henghold, W.B. Other biologic toxin bioweapons: ricin, staphylococcal enterotoxin B, and trichothecene mycotoxins. Dermatol. Clin. 2004, 22 (3), 257-262.
21. Lam, M.T.; Boulet, C.A.; Le, X.C. Development of a tetramethylrhodamine- labeled probe for a capillary electrophoresis-based competitive immunoassay of staphylococcal enterotoxin B. Anal. Chim. Acta 2002, 457 (1), 21-28.
22. Lam, M.T.; Wan, Q.H.; Boulet, C.A.; Le, X.C. Competitive immunoassay for staphylococcal enterotoxin A using capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A 1999, 853 (1-2), 545-553.
23. Ewalt, K.L.; Haigis, R.W.; Rooney, R.; Ackley, D.; Krihak, M. Detection of biological toxins on an active electronic microchip. Anal. Biochem. 2001, 289 (2), 162-172.