Purification and characterization of a Bacillus circulans No. 4.1 chitinase expressed in Escherichia coli

World Journal of Microbiology and Biotechnology

Volumn 22, Issue 4, 2006, Pages 331-335

  Siwayaprahm, Patcharaporn ^a   Audtho, Mongkon ^b   Ohmiya, Kunio ^c   Wiwat, Chanpen ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^c MIE UNIVERSITY   (Japan)

Author keywords

Bacillus circulans No. 4.1; Characterization; Chitin; Chitinase; Purification

Indexed keywords

AMINO ACIDS; CHARACTERIZATION; CHELATION; CHITIN; ESCHERICHIA COLI; PROTEINS; PURIFICATION; RESINS;

BACILLUS CIRCULANS NO. 4.1; CHITINASE; GLYCOL CHITIN; N-ACETYLCHITOOLIGOSACCHARIDES;

DNA;

BACILLUS CIRCULANS; ESCHERICHIA COLI;

EID: 33646442183     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-005-9038-8     Document Type: Article

References (15)

1. Dow, J.A.T. 1992 pH gradient in lepidopteran midgut. Journal of Experimental Biology 172, 355-375.
2. Hudson, L. & Hay, F.C. 1980 Practical Immunology, 2nd edition, pp. 124-128, Oxford: Blackwell Scientific Publications, ISBN 0632003537.
3. Hwang, J. & Wang, S. 2001 Microbial reclamation of shellfish wastes for the production of chitinases. Enzyme and Microbial Technology 28, 376-382.
4. Imoto, T. & Yagishita, K. 1971 A simple activity measurement of lysozyme. Agricultural and Biological Chemistry 35, 1154-1156.
5. Morimoto, K., Karita, S., Kimura, T., Sakka, K. & Ohmiya, K. 2001 Characterization of Clostridium paraputrificum chitinase A from a recombinant Escherichia coli. Journal of Bioscience and Bioengneering 92, 466-468.
6. O'Brien, M. & Colwell, R.R. 1987 A rapid test chitinase activity that uses 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide. Applied and Environmental Microbiology 53, 1718-1720.
7. Takayanagi, T., Ajisaka, K. & Takiguchi, Y. 1991 Isolation and characterization of thermostable chitinases from Bacillus licheniformis X-7u. Biochimica et Biophysica Acta 1078, 404-410.
8. Thamthiankul, S., Suan-ngay, S., Tantimavanich, S. & Panbangred, W. 2001 Chitinase from Bacillus thuringiensis subsp. pakistani. Applied Microbiology and Biotechnology 56, 395-401.
9. Tronsmo, A. & Harman, G.E. 1976 Detection and Quantification of N-acetyl-β-D-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels. Analytical Biochemistry 208, 74-79.
10. Ueda, M., Fujiwara, A., Kawaguchi, T. & Arai, M. 1995 Purification and some properties of six chitinases from Aeromonas sp. no. 10S-24. Bioscience Biotechnology and Biochemistry 59, 2162-2164.
11. Watanabe, T., Yamada, T., Oyanagi, W., Suzuki, K. & Tanaka, H. 1992 Purification and some properties of chitinase B1 from Bacillus circulans WL-12. Bioscience Biotechnology and Biochemistry 56, 682-683.
12. Wiwat, C., Lertcanawanichakul, M., Siwayaprahm, P., Pantuwatana, S. & Bhumiratana, A. 1996 Expression of chitinase-encoding genes from Aeromonas hydrophila and Pseudomonas maltophila in Bacillus thuringiensis subsp. israelensis. Gene 197, 119-126.
13. Wiwat, C., Panbangred, W., Mongkolsuk, S., Pantuwatana, S. & Bhumiratana, A. 1995 Inhibition of a conjugation-like gene transfer process in Bacillus thuringiensis subsp. israelensis by the anti-s layer protein antibody. Current Microbiology 30, 69-75.
14. Wiwat, C., Siwayaprahm, P. & Bhumiratana, A. 1999 Purification and characterization of chitinase from Bacillus circulans No. 4.1. Current Microbiology 39, 134-140.
15. Wiwat, C., Thepouyporn, A., Siwayaprahm, P. & Bhumiratana, A. 2002 Cloning, sequencing, and expression of a chitinase-encoding gene from Bacillus circulans No. 4.1. Current Microbiology 44, 167-172.