Use of Western blotting filtration to detect UV-cross-linked protein: RNA complexes

Analytical Biochemistry

Volumn 353, Issue 1, 2006, Pages 138-140

  Espel, Enric ^a   Teixé, Trini ^a   Reina, Manuel ^a   Vilaró, Senén ^a   Parra, Josep Lluis ^b   Buxadé, Maria ^b  

^a UNIVERSITY OF BARCELONA   (Spain)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

CROSS-LINKED PROTEINS; WESTERN BLOTTING;

CHEMISTRY;

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INITIATION FACTOR 4E; PROTEIN; RIBONUCLEASE; RNA; RNA BINDING PROTEIN; TUMOR NECROSIS FACTOR ALPHA;

ANALYTIC METHOD; ARTICLE; FILTRATION; GEL MOBILITY SHIFT ASSAY; PHOTOAFFINITY LABELING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN RNA BINDING; RNA BINDING; ULTRAVIOLET RADIATION; WESTERN BLOTTING;

EID: 33646384127     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.11.026     Document Type: Article

References (15)

1. Dreyfuss G., Choi Y.D., and Adam S.A. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies. Mol. Cell Biol. 4 (1984) 1104-1114
2. Hanna M.M. Photoaffinity cross-linking methods for studying RNA-protein interactions. Methods Enzymol. 180 (1989) 383-409
3. Yarus M., and Berg P. On the properties and utility of a membrane filter assay in the study of isoleucyl-tRNA synthetase. Anal. Biochem. 35 (1970) 450-465
4. Setzer D.R. Measuring equilibrium and kinetic constants using gel retardation assays. Methods Mol. Biol. 118 (1999) 115-128
5. Wilson G.M., and Brewer G. Identification and characterization of proteins binding A + U-rich elements. Methods 17 (1999) 74-83
6. Krecic A.M., and Swanson M.S. hnRNP complexes: composition, structure, and function. Curr. Opin. Cell Biol. 11 (1999) 363-371
7. Shyu A.B., and Wilkinson M.F. The double lives of shuttling mRNA binding proteins. Cell 102 (2000) 135-138
8. Rajagopalan L.E., and Malter J.S. Modulation of granulocyte-macrophage colony-stimulating factor mRNA stability in vitro by the adenosine-uridine binding factor. J. Biol. Chem. 269 (1994) 23882-23888
9. Hamilton B.J., Burns C.M., Nichols R.C., and Rigby W.F. Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J. Biol. Chem. 272 (1997) 28732-28741
10. Buxade M., Parra J.L., Rousseau S., Shpiro N., Marquez R., Morrice N., Bain J., Espel E., and Proud C.G. The Mnks are novel components in the control of TNFalpha biosynthesis and phosphorylate and regulate hnRNP A1. Immunity 23 (2005) 177-189
11. Leibold E.A., and Munro H.N. Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5′ untranslated region of ferritin heavy- and light-subunit mRNAs. Proc. Natl. Acad. Sci. USA 85 (1988) 2171-2175
12. Katz D.A., Theodorakis N.G., Cleveland D.W., Lindsten T., and Thompson C.B. AU-A, an RNA-binding activity distinct from hnRNP A1, is selective for AUUUA repeats and shuttles between the nucleus and the cytoplasm. Nucleic Acids Res. 22 (1994) 238-246
13. Gingras A.-C., Raught B., and Sonenberg N. eIF4 initiation factors:effectors of mRNA recruitment to ribosomes and regulators of translation. Annu. Rev. Biochem. 68 (1999) 913-963
14. Bickel M., Iwai Y., Pluznik D.H., and Cohen R.B. Binding of sequence-specific proteins to the adenosine-plus uridine-rich sequences of the murine granulocyte/macrophage colony-stimulating factor mRNA. Proc. Natl. Acad. Sci. USA 89 (1992) 10001-10005
15. Novak U., and Paradiso L. Identification of proteins in DNA-protein complexes after blotting of EMSA gels. Biotechniques 19 (1995) 54-55